Transcriptome Profiling Reveals Distinctive Traits of Retinol Metabolism and Neonatal Parallels in the MRL/MpJ Mouse

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2015 Nov 18

PMID 26572684

Citations 9

Authors

Justyna Podolak-Popinigis

Bartosz Gornikiewicz

Anna Ronowicz

Pawel Sachadyn

Affiliations

Soon will be listed here.

Abstract

Background: The MRL/MpJ mouse is a laboratory inbred strain known for regenerative abilities which are manifested by scarless closure of ear pinna punch holes. Enhanced healing responses have been reported in other organs. A remarkable feature of the strain is that the adult MRL/MpJ mouse retains several embryonic biochemical characteristics, including increased expression of stem cell markers.

Results: We explored the transcriptome of the MRL/MpJ mouse in the heart, liver, spleen, bone marrow and ears. We used two reference strains, thus increasing the chances to discover the genes responsible for the exceptional properties of the regenerative strain. We revealed several distinctive characteristics of gene expression patterns in the MRL/MpJ mouse, including the repression of immune response genes, the up-regulation of those associated with retinol metabolism and PPAR signalling, as well as differences in expression of the genes engaged in wounding response. Another crucial finding is that the gene expression patterns in the adult MRL/MpJ mouse and murine neonates share a number of parallels, which are also related to immune and wounding response, PPAR pathway, and retinol metabolism.

Conclusions: Our results indicate the significance of retinol signalling and neonatal transcriptomic relics as the distinguishing features of the MRL/MpJ mouse. The possibility that retinoids could act as key regulatory molecules in this regeneration model brings important implications for regenerative medicine.

Citing Articles

Intersection of Immunity, Metabolism, and Muscle Regeneration in an Autoimmune-Prone MRL Mouse Model.

Ngo T, Josyula A, DeStefano S, Fertil D, Faust M, Lokwani R Adv Sci (Weinh). 2024; 11(11):e2306961.

PMID: 38192168 PMC: 10953568. DOI: 10.1002/advs.202306961.

Integrated Transcriptome and Metabolomics to Reveal the Mechanism of Adipose Mesenchymal Stem Cells in Treating Liver Fibrosis.

Liu H, Wang X, Deng H, Huang H, Liu Y, Zhong Z Int J Mol Sci. 2023; 24(22).

PMID: 38003277 PMC: 10671340. DOI: 10.3390/ijms242216086.

Ectopic adipogenesis in response to injury and material implantation in an autoimmune mouse model.

Ngo T, Josyula A, DeStefano S, Fertil D, Faust M, Lokwani R bioRxiv. 2023; .

PMID: 37986843 PMC: 10659416. DOI: 10.1101/2023.10.05.561105.

Regenerative Drug Discovery Using Ear Pinna Punch Wound Model in Mice.

Sosnowski P, Sass P, Slonimska P, Platek R, Kaminska J, Baczynski Keller J Pharmaceuticals (Basel). 2022; 15(5).

PMID: 35631437 PMC: 9145447. DOI: 10.3390/ph15050610.

Thirty Mouse Strain Survey of Voluntary Physical Activity and Energy Expenditure: Influence of Strain, Sex and Day-Night Variation.

Konig C, Plank A, Kapp A, Timotius I, von Horsten S, Zimmermann K Front Neurosci. 2020; 14:531.

PMID: 32733181 PMC: 7358574. DOI: 10.3389/fnins.2020.00531.

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