Comparison of the Pasteurella Haemolytica A1 Envelope Proteins Obtained by Two Cell Disruption Methods

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1989 Apr 1

PMID 2656741

Citations 3

Authors

K R Simons

R J Morton

D A Mosier

R W Fulton

A W Confer

Affiliations

Soon will be listed here.

Abstract

The French pressure cell and sonication methods of bacterial cell disruption were compared for the evaluation of surface proteins from Pasteurella haemolytica A1. Several protein bands were quantitatively different when compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and densitometry. With densitometry, sonicated preparations had higher concentrations of the 102-, 83-, 50-, 38-, and 30-kilodalton (kDa) proteins; French pressure cell preparations had higher concentrations of the 96-, 65-, and 42-kDa proteins. Qualitative differences between these two disruption methods were evident at the 102-, 96-, 91-, 50-, 38-, and 30-kDa protein bands. However, significant differences (P less than 0.05) were detected between the two methods at only the 102-, 96-, 91-, and 50-kDa bands.

Citing Articles

Comparison of pig, rabbit and mouse IgG response to Streptococcus suis serotype 2 proteins and active immunization of mice against the infection.

Quessy S, Dubreuil J, Caya M, Letourneau R, Higgins R Can J Vet Res. 1994; 58(3):220-3.

PMID: 7954125 PMC: 1263699.

DNA probes for diagnosis of intestinal infection.

Char S, Farthing M Gut. 1991; 32(1):1-3.

PMID: 1991625 PMC: 1379202. DOI: 10.1136/gut.32.1.1.

Co-migrating and shared antigens of selected Pasteurella haemolytica untypable strains.

Simons K, Morton R, Mosier D, Fulton R, Confer A Vet Res Commun. 1992; 16(3):177-83.

PMID: 1413477 DOI: 10.1007/BF01839153.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Newman P, Corstvet R, Panciera R . Distribution of Pasteurella haemolytica and Pasteurella multocida in the bovine lung following vaccination and challenge exposure as an indicator of lung resistance. Am J Vet Res. 1982; 43(3):417-22. View

Hansen E, Frisch C, McDade Jr R, Johnston K . Identification of immunogenic outer membrane proteins of Haemophilus influenzae type b in the infant rat model system. Infect Immun. 1981; 32(3):1084-92. PMC: 351562. DOI: 10.1128/iai.32.3.1084-1092.1981. View

Blaser M, Hopkins J, Berka R, Vasil M, Wang W . Identification and characterization of Campylobacter jejuni outer membrane proteins. Infect Immun. 1983; 42(1):276-84. PMC: 264555. DOI: 10.1128/iai.42.1.276-284.1983. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Gilmour N, Angus K, Donachie W, Fraser J . Vaccination against experimental pneumonic pasteurellosis. Vet Rec. 1982; 110(19):450. DOI: 10.1136/vr.110.19.450-a. View

Purdy C, LIVINGSTON Jr C, Frank G, Cummins J, Cole N, Loan R . A live Pasteurella haemolytica vaccine efficacy trial. J Am Vet Med Assoc. 1986; 188(6):589-91. View

Horsnell J, Teale C . Characterisation of the outer membrane protein antigens of British field isolates of Moraxella bovis. Vet Microbiol. 1987; 15(3):181-9. DOI: 10.1016/0378-1135(87)90072-1. View

Afzal M, BRODIE S, Tengerdy R, SQUIRE P . Isolation and antigenic reactivity of Brucella ovis outer membrane proteins. J Clin Microbiol. 1987; 25(11):2132-5. PMC: 269426. DOI: 10.1128/jcm.25.11.2132-2135.1987. View

10.

Confer A, Panciera R, Fulton R, Gentry M, Rummage J . Effect of vaccination with live or killed Pasteurella haemolytica on resistance to experimental bovine pneumonic pasteurellosis. Am J Vet Res. 1985; 46(2):342-7. View

11.

Dunn B, Blaser M, Snyder E . Two-dimensional gel electrophoresis and immunoblotting of Campylobacter outer membrane proteins. Infect Immun. 1987; 55(7):1564-72. PMC: 260559. DOI: 10.1128/iai.55.7.1564-1572.1987. View

12.

Lillie L . The bovine respiratory disease complex. Can Vet J. 1974; 15(9):233-42. PMC: 1696627. View

13.

SQUIRE P, Smiley D, Croskell R . Identification and extraction of Pasteurella haemolytica membrane proteins. Infect Immun. 1984; 45(3):667-73. PMC: 263347. DOI: 10.1128/iai.45.3.667-673.1984. View

14.

Wilkie B, Markham R, Shewen P . Response of calves to lung challenge exposure with Pasteurella haemolytica after parenteral or pulmonary immunization. Am J Vet Res. 1980; 41(11):1773-8. View

15.

Yates W, Stockdale P, Babiuk L, Smith R . Prevention of experimental bovine pneumonic pasteurellosis with an extract of Pasteurella haemolytica. Can J Comp Med. 1983; 47(3):250-6. PMC: 1235934. View

16.

Martin S . Vaccination: Is it Effective in Preventing Respiratory Disease or Influencing Weight Gains in Feedlot Calves?. Can Vet J. 1983; 24(1):10-9. PMC: 1790242. View