Identification of Immunogenic Outer Membrane Proteins of Haemophilus Influenzae Type B in the Infant Rat Model System

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1981 Jun 1

PMID 6972915

Citations 55

Authors

E J Hansen

C F Frisch

R L McDade Jr

K H Johnston

Affiliations

Soon will be listed here.

Abstract

Outer membrane proteins of Haemophilus influenzae type b which are immunogenic in infant rats were identified by a radioimmunoprecipitation method. Intact cells of H. influenzae type b were radioiodinated by a lactoperoxidase-catalyzed procedure, and an outer membrane-containing fraction was prepared from these cells. These radioiodinated outer membranes were mixed with sera obtained from rats convalescing from systemic H. influenzae type b disease induced at 6 days of age, and the resultant (antibody-outer membrane protein antigen) complexes were extracted from these membranes by treatment with nonionic detergent and ethylenediaminetetraacetic acid. These soluble antibody-antigen complexes were isolated by means of adsorption to protein A-bearing staphylococci, and the radioiodinated protein antigens were identified by gel electrophoresis followed by autoradiography. Infant rats were shown to mount a readily detectable antibody response to several different proteins present in the outer membrane of H. influenzae type b. Individual infant rats were found to vary both qualitatively and quantitatively in their immune response to these immunogenic outer membrane proteins.

Citing Articles

Identification of an outer membrane protein involved in utilization of hemoglobin-haptoglobin complexes by nontypeable Haemophilus influenzae.

Maciver I, Latimer J, LIEM H, Muller-Eberhard U, Hrkal Z, Hansen E Infect Immun. 1996; 64(9):3703-12.

PMID: 8751920 PMC: 174284. DOI: 10.1128/iai.64.9.3703-3712.1996.

Pathogenesis and pathophysiology of bacterial meningitis.

Tunkel A, Scheld W Clin Microbiol Rev. 1993; 6(2):118-36.

PMID: 8472245 PMC: 358273. DOI: 10.1128/CMR.6.2.118.

Antibodies to Haemophilus influenzae type b outer membrane proteins in children with epiglottitis or meningitis and in healthy controls.

Johnson P, MacInnes S, Gilbert G Infect Immun. 1993; 61(4):1531-7.

PMID: 8454359 PMC: 281396. DOI: 10.1128/iai.61.4.1531-1537.1993.

Antibody response of infants to cell surface-exposed outer membrane proteins of Haemophilus influenzae type b after systemic Haemophilus disease.

Gulig P, McCracken Jr G, Frisch C, Johnston K, Hansen E Infect Immun. 1982; 37(1):82-8.

PMID: 6980838 PMC: 347494. DOI: 10.1128/iai.37.1.82-88.1982.

Detection of antibody-accessible proteins on the cell surface of Haemophilus influenzae type b.

Hansen E, Frisch C, Johnston K Infect Immun. 1981; 33(3):950-3.

PMID: 6974700 PMC: 350803. DOI: 10.1128/iai.33.3.950-953.1981.

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