Anti-miroestrol Polyclonal Antibodies: a Comparison of Immunogen Preparations Used to Obtain Desired Antibody Properties

Overview

Journal J Nat Med

Publisher Springer

Date 2015 Nov 14

PMID 26563142

Citations 5

Authors

Tharita Kitisripanya

Kamonthip Jutathis

Chadathorn Inyai

Jukrapun Komaikul

Orapin Udomsin

Gorawit Yusakul

Hiroyuki Tanaka

Waraporn Putalun

Affiliations

Soon will be listed here.

Abstract

Immunogen quality is one important factor that contributes to desirable antibody characteristics. Highly specific antibodies against miroestrol can be used to develop a quality control immunoassay for Pueraria candollei products. In this study, we investigated how various immunogen preparations affect antibody properties. The results show that immunogen prepared using the Mannich reaction provides antibodies with higher specificity and sensitivity against miroestrol than immunogen prepared with the periodate reaction. The results suggest the Mannich reaction maintains the original structure of miroestrol and generates useful antibodies for developing immunoassays.

Citing Articles

Comparative stability and analytical performance of anti-miroestrol recombinant antibody in different cassettes.

Sae-Foo W, Yusakul G, Kitisripanya T, Nuntawong P, Sakamoto S, Putalun W Appl Microbiol Biotechnol. 2023; 107(9):2887-2896.

PMID: 36995382 DOI: 10.1007/s00253-023-12492-6.

Predictable Peptide Conjugation Ratios by Activation of Proteins with Succinimidyl Iodoacetate (SIA).

Abbas I, Schwaar T, Bienwald F, Weller M Methods Protoc. 2019; 1(1).

PMID: 31164550 PMC: 6526413. DOI: 10.3390/mps1010002.

Enzyme linked immunosorbent assay for total potent estrogenic miroestrol and deoxymiroestrol of Pueraria candollei, a Thai herb for menopause remedy.

Yusakul G, Kitisripanya T, Juengwatanatrakul T, Sakamoto S, Tanaka H, Putalun W J Nat Med. 2018; 72(3):641-650.

PMID: 29492802 DOI: 10.1007/s11418-018-1194-x.

Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites.

Sakamoto S, Putalun W, Vimolmangkang S, Phoolcharoen W, Shoyama Y, Tanaka H J Nat Med. 2017; 72(1):32-42.

PMID: 29164507 PMC: 5775980. DOI: 10.1007/s11418-017-1144-z.

A lateral flow colloidal gold-based immunoassay for rapid detection of miroestrol in samples of White Kwao Krua, a phytoestrogen-rich plant.

Kitisripanya T, Inyai C, Komaikul J, Krittanai S, Juengwatanatrakul T, Sakamoto S J Nat Med. 2017; 71(4):659-664.

PMID: 28573485 DOI: 10.1007/s11418-017-1096-3.

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