Microbial Production of Scleroglucan and Downstream Processing

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2015 Nov 4

PMID 26528259

Citations 15

Authors

Natalia A Castillo

Alejandra L Valdez

Julia I Farina

Affiliations

Soon will be listed here.

Abstract

Synthetic petroleum-based polymers and natural plant polymers have the disadvantage of restricted sources, in addition to the non-biodegradability of the former ones. In contrast, eco-sustainable microbial polysaccharides, of low-cost and standardized production, represent an alternative to address this situation. With a strong global market, they attracted worldwide attention because of their novel and unique physico-chemical properties as well as varied industrial applications, and many of them are promptly becoming economically competitive. Scleroglucan, a β-1,3-β-1,6-glucan secreted by Sclerotium fungi, exhibits high potential for commercialization and may show different branching frequency, side-chain length, and/or molecular weight depending on the producing strain or culture conditions. Water-solubility, viscosifying ability and wide stability over temperature, pH and salinity make scleroglucan useful for different biotechnological (enhanced oil recovery, food additives, drug delivery, cosmetic and pharmaceutical products, biocompatible materials, etc.), and biomedical (immunoceutical, antitumor, etc.) applications. It can be copiously produced at bioreactor scale under standardized conditions, where a high exopolysaccharide concentration normally governs the process optimization. Operative and nutritional conditions, as well as the incidence of scleroglucan downstream processing will be discussed in this chapter. The relevance of using standardized inocula from selected strains and experiences concerning the intricate scleroglucan scaling-up will be also herein outlined.

Citing Articles

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Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to β-glucans.

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Carboxymethyl Scleroglucan Synthesized via -Alkylation Reaction with Different Degrees of Substitution: Rheology and Thermal Stability.

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Experimental Investigation of the Viscosity and Stability of Scleroglucan-Based Nanofluids for Enhanced Oil Recovery.

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PMID: 38251121 PMC: 10818491. DOI: 10.3390/nano14020156.

References

Gibbs P, Seviour R, Schmid F . Growth of filamentous fungi in submerged culture: problems and possible solutions. Crit Rev Biotechnol. 2000; 20(1):17-48. DOI: 10.1080/07388550091144177. View

Jong S, DONOVICK R . Antitumor and antiviral substances from fungi. Adv Appl Microbiol. 1989; 34:183-262. DOI: 10.1016/s0065-2164(08)70319-8. View

Wucherpfennig T, Kiep K, Driouch H, Wittmann C, Krull R . Morphology and rheology in filamentous cultivations. Adv Appl Microbiol. 2010; 72:89-136. DOI: 10.1016/S0065-2164(10)72004-9. View

Garcia-Ochoa F, Gomez E . Bioreactor scale-up and oxygen transfer rate in microbial processes: an overview. Biotechnol Adv. 2008; 27(2):153-76. DOI: 10.1016/j.biotechadv.2008.10.006. View

Zheng Z, Wang S, Li G, Zhan X, Lin C, Wu J . A new polysialic acid production process based on dual-stage pH control and fed-batch fermentation for higher yield and resulting high molecular weight product. Appl Microbiol Biotechnol. 2012; 97(6):2405-12. DOI: 10.1007/s00253-012-4503-4. View

Martin K, McDougall B, McIlroy S, Chen J, Seviour R . Biochemistry and molecular biology of exocellular fungal beta-(1,3)- and beta-(1,6)-glucanases. FEMS Microbiol Rev. 2007; 31(2):168-92. DOI: 10.1111/j.1574-6976.2006.00055.x. View

Farina J, Vinarta S, Cattaneo M, Figueroa L . Structural stability of Sclerotium rolfsii ATCC 201126 beta-glucan with fermentation time: a chemical, infrared spectroscopic and enzymatic approach. J Appl Microbiol. 2008; 106(1):221-32. DOI: 10.1111/j.1365-2672.2008.03995.x. View

Allen D, Robinson C . Hydrodynamics and mass transfer in Aspergillus niger fermentations in bubble column and loop bioreactors. Biotechnol Bioeng. 1989; 34(6):731-40. DOI: 10.1002/bit.260340602. View

Singh P, WHISTLER R, TOKUZEN R, Nakahara W . Scleroglucan, an antitumor polysaccharide from Sclerotium glucanicum. Carbohydr Res. 1974; 37(1):245-7. DOI: 10.1016/s0008-6215(00)87078-0. View

10.

Leal F, Ruiz-Herrera J, VILLANUEVA J, Larriba G . An examination of factors affecting the instability of Saccharomyces cerevisiae glucan synthetase in cell free extracts. Arch Microbiol. 1984; 137(3):209-14. DOI: 10.1007/BF00414545. View

11.

Papagianni M . Fungal morphology and metabolite production in submerged mycelial processes. Biotechnol Adv. 2003; 22(3):189-259. DOI: 10.1016/j.biotechadv.2003.09.005. View

12.

Bielecki S, GALAS E . Microbial beta-glucanases different from cellulases. Crit Rev Biotechnol. 1991; 10(4):275-304. DOI: 10.3109/07388559109038212. View

13.

Pretus H, Ensley H, McNamee R, Jones E, Browder I, Williams D . Isolation, physicochemical characterization and preclinical efficacy evaluation of soluble scleroglucan. J Pharmacol Exp Ther. 1991; 257(1):500-10. View

14.

Miller R, Liberta A . The effect of light on acid-soluble polysaccharide accumulation in Sclerotium rolfsii Sacc. Can J Microbiol. 1976; 22(7):967-70. DOI: 10.1139/m76-140. View

15.

Kim Y, Kim E, Cheong C, Williams D, Kim C, Lim S . Structural characterization of beta-D-(1 --> 3, 1 --> 6)-linked glucans using NMR spectroscopy. Carbohydr Res. 2000; 328(3):331-41. DOI: 10.1016/s0008-6215(00)00105-1. View

16.

Rice P, Lockhart B, Barker L, Adams E, Ensley H, Williams D . Pharmacokinetics of fungal (1-3)-beta-D-glucans following intravenous administration in rats. Int Immunopharmacol. 2004; 4(9):1209-15. DOI: 10.1016/j.intimp.2004.05.013. View

17.

Coviello T, Grassi M, Rambone G, Santucci E, Carafa M, Murtas E . Novel hydrogel system from scleroglucan: synthesis and characterization. J Control Release. 1999; 60(2-3):367-78. DOI: 10.1016/s0168-3659(99)00091-7. View

18.

Vinarta S, Delgado O, Figueroa L, Farina J . Effects of thermal, alkaline and ultrasonic treatments on scleroglucan stability and flow behavior. Carbohydr Polym. 2013; 94(1):496-504. DOI: 10.1016/j.carbpol.2013.01.063. View

19.

Stasinopoulos S, Seviour R . Stimulation of exopolysaccharide production in the fungus Acremonium persicinum with fatty acids. Biotechnol Bioeng. 1990; 36(8):778-82. DOI: 10.1002/bit.260360804. View

20.

Schmid J, Meyer V, Sieber V . Scleroglucan: biosynthesis, production and application of a versatile hydrocolloid. Appl Microbiol Biotechnol. 2011; 91(4):937-47. DOI: 10.1007/s00253-011-3438-5. View