Efficient Production of Scleroglucan by and Insights Into Molecular Weight Modification by High-Pressure Homogenization

Overview

Journal Front Bioeng Biotechnol

Date 2021 Sep 20

PMID 34540818

Citations 2

Authors

Weizhu Zeng

Junyi Wang

Xiaoyu Shan

Shiqin Yu

Jingwen Zhou

Affiliations

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Abstract

Scleroglucan is a non-ionic water-soluble polysaccharide, and has been widely used in the petroleum, food, medicine and cosmetics industries. Currently, scleroglucan is mainly produced by . A higher level of scleroglucan (42.0 g/L) was previously obtained with WSH-G01. However, the production of scleroglucan was reduced despite a higher glucose concentration remaining. Additionally, the molecular weight of scleroglucan was large, thus restricted its application. In this study, by adjusting the state of seeds inoculated, the degradation issue of scleroglucan during the fermentation process was solved. By comparing different fed-batch strategies, 66.6 g/L of scleroglucan was harvested by a two-dose fed-batch mode, with 53.3% glucose conversion ratio. To modify the molecular weight of scleroglucan, a combination method with HCl and high-pressure homogenization treatment was established. Finally, scleroglucan with molecular weight of 4.61 × 10 Da was obtained. The developed approaches provide references for the biosynthesis and molecular weight modification of polysaccharides.

Citing Articles

Synthesis and Characterization of New Nanohybrids Based on Carboxymethyl Scleroglucan and Silica Nanoparticles.

Castro R, Corredor L, Burgos I, Llanos S, Franco C, Cortes F Nanomaterials (Basel). 2024; 14(6).

PMID: 38535647 PMC: 10974519. DOI: 10.3390/nano14060499.

Carboxymethyl Scleroglucan Synthesized via -Alkylation Reaction with Different Degrees of Substitution: Rheology and Thermal Stability.

Castro R, Burgos I, Corredor L, Llanos S, Franco C, Cortes F Polymers (Basel). 2024; 16(2).

PMID: 38257006 PMC: 10821296. DOI: 10.3390/polym16020207.

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