A Pilot Study to Assess Adenosine 5'-triphosphate Metabolism in Red Blood Cells As a Drug Target for Potential Cardiovascular Protection

Overview

Journal Cardiovasc Hematol Disord Drug Targets

Publisher Bentham Science Publishers

Specialties Cardiology & Vascular Diseases
Hematology
Pharmacology

Date 2015 Nov 3

PMID 26521884

Citations 2

Authors

Pollen K F Yeung

Jodi Tinkel

Dena Seeto

Affiliations

Soon will be listed here.

Abstract

Objective: To study the effect of exercise preconditioning on adenosine 5'triphosphate (ATP) metabolism in red blood cells and cardiovascular protection against injury induced by isoproterenol in vivo.

Methods: Male Sprague Dawley rats (SDR) were each exercised on a treadmill for 15 minutes at 10 m/min and 10% grade (n = 7) (LowEx), or 14 m/min and 22% grade (n = 8) (VigEx). Two hours after the exercise, each rat received a single dose of isoproterenol (30 mg/kg) by subcutaneous (sc) injection. Two separate groups of SDR were used as control: One received no exercise (n = 10) (NoEx) and the other received no exercise and no isoproterenol (n = 11) (NoIso). Serial blood samples were collected over 5 hours for measurement of ATP and its catabolites by a validated HPLC. Hemodynamic recording was collected continuously for the duration of the experiment. Data were analysed using ANOVA and t-tests and difference considered significant at p < 0.05.

Results: Exercise pre-conditioning (both LowEx and VigEx) reduced mortality after isoproterenol from 50% to < 30% (p > 0.05). It attenuated the rebound in blood pressure significantly (p < 0.05 between NoEx vs VigEx), attenuated the increase of RBC adenosine 5'-monophosphate (AMP) concentrations induced by isoproterenol, and also decreased the breakdown of ATP to AMP in the RBC (p < 0.05 vs NoEx).

Conclusion: Exercise pre-conditioning decreased the blood pressure rebound and also breakdown of ATP in RBC after isoproterenol which may be exploited further as a drug target for cardiovascular protection and prevention.

Citing Articles

Post-Exercise Hypotension: An Alternative Management Strategy for Hypertension and Cardiovascular Disease?.

Aly K, Yeung P J Clin Med. 2023; 12(13).

PMID: 37445491 PMC: 10342491. DOI: 10.3390/jcm12134456.

Adenosine 5'-Triphosphate Metabolism in Red Blood Cells as a Potential Biomarker for Post-Exercise Hypotension and a Drug Target for Cardiovascular Protection.

Yeung P, Kolathuru S, Mohammadizadeh S, Akhoundi F, Linderfield B Metabolites. 2018; 8(2).

PMID: 29724022 PMC: 6027528. DOI: 10.3390/metabo8020030.

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