Exercise Preconditioning Initiates Late Cardioprotection Against Isoproterenol-induced Myocardial Injury in Rats Independent of Protein Kinase C

Overview

Journal J Physiol Sci

Specialty Physiology

Date 2010 Oct 14

PMID 20941560

Citations 15

Authors

Yu-Jun Shen

Shan-Shan Pan

Tao Zhuang

Feng-Juan Wang

Affiliations

Soon will be listed here.

Abstract

The objective of this study was to investigate the late cardioprotective effect of exercise preconditioning (EP) on isoproterenol (ISO)-induced myocardial injury in rats and the role of protein kinase C (PKC) in EP. Rats were injected with ISO 24 h after running on a treadmill for four periods of 10 min each at 28-30 m/min with intervening periods of rest of 10 min. Nonselective PKC inhibitor chelerythrine (CHE) was injected before EP. The myocardial injury was evaluated quantitatively in terms of the serum cardiac troponin I (cTnI) levels, the myocardial ischemia/hypoxia area, and the integral optical density (IOD) of haematoxylin-basic fuchsin-picric acid (HBFP) staining, and qualitatively in terms of the myocardial ultrastructure. EP markedly attenuated the ISO-induced myocardial ischemia/hypoxia and ultrastructural damage with lower serum cTnI levels. CHE injection before EP did not block the protective effect of EP, displaying a mild myocardial ischemia/hypoxia and well-preserved ultrastructure with even lower serum cTnI levels. The results indicate that EP can exert a late cardioprotection against ISO-induced myocardial injury, and that an injection of the nonselective PKC inhibitor CHE before EP may have a different effect on ISO-induced myocardial injury. Further investigation needs to be conducted to define the role of different PKC isozymes in EP by using isozyme-selective inhibitors.

Citing Articles

Exercise preconditioning promotes myocardial GLUT4 translocation and induces autophagy to alleviate exhaustive exercise-induced myocardial injury in rats.

Guo Y, Pan S, Chen T, Huang Y, Wan D, Tong Y J Mol Histol. 2023; 54(5):453-472.

PMID: 37715078 DOI: 10.1007/s10735-023-10152-7.

Exercise preconditioning improves electrocardiographic signs of myocardial ischemic/hypoxic injury and malignant arrhythmias occurring after exhaustive exercise in rats.

Guo Y, Pan S Sci Rep. 2022; 12(1):18772.

PMID: 36335157 PMC: 9637115. DOI: 10.1038/s41598-022-23466-5.

Exercise-induced signaling pathways to counteracting cardiac apoptotic processes.

Pahlavani H Front Cell Dev Biol. 2022; 10:950927.

PMID: 36036015 PMC: 9403089. DOI: 10.3389/fcell.2022.950927.

Voluntary exercise and cardiac remodeling in a myocardial infarction model.

Al Shahi H, Kadoguchi T, Shimada K, Fukao K, Matsushita S, Aikawa T Open Med (Wars). 2020; 15(1):545-555.

PMID: 33313409 PMC: 7706131. DOI: 10.1515/med-2020-0109.

Altered expression levels of autophagy-associated proteins during exercise preconditioning indicate the involvement of autophagy in cardioprotection against exercise-induced myocardial injury.

Yuan J, Yuan Y, Pan S, Cai K J Physiol Sci. 2020; 70(1):10.

PMID: 32066368 PMC: 7026234. DOI: 10.1186/s12576-020-00738-1.

References

Wisloff U, Stoylen A, Loennechen J, Bruvold M, Rognmo O, Haram P . Superior cardiovascular effect of aerobic interval training versus moderate continuous training in heart failure patients: a randomized study. Circulation. 2007; 115(24):3086-94. DOI: 10.1161/CIRCULATIONAHA.106.675041. View

Rona G, KAHN D, Chappel C . STUDIES ON INFARCT-LIKE MYOCARDIAL NECROSIS PRODUCED BY ISOPROTERENOL: A REVIEW. Rev Can Biol. 1963; 22:241-55. View

Dong F, Taylor M, Samson W, Ren J . Intermedin (adrenomedullin-2) enhances cardiac contractile function via a protein kinase C- and protein kinase A-dependent pathway in murine ventricular myocytes. J Appl Physiol (1985). 2006; 101(3):778-84. DOI: 10.1152/japplphysiol.01631.2005. View

French J, Hamilton K, Quindry J, Lee Y, Upchurch P, Powers S . Exercise-induced protection against myocardial apoptosis and necrosis: MnSOD, calcium-handling proteins, and calpain. FASEB J. 2008; 22(8):2862-71. PMC: 2493460. DOI: 10.1096/fj.07-102541. View

Johnson J, Gray M, Chen C, Mochly-Rosen D . A protein kinase C translocation inhibitor as an isozyme-selective antagonist of cardiac function. J Biol Chem. 1996; 271(40):24962-6. DOI: 10.1074/jbc.271.40.24962. View

Scherer A, MASI A . Technical aspects of the Haematoxylin--Basic Fuchsin--picric acid (HBFP) stain applied to skeletal muscle. Histochem J. 1975; 7(4):335-41. DOI: 10.1007/BF01007018. View

Gross G, Fryer R . Sarcolemmal versus mitochondrial ATP-sensitive K+ channels and myocardial preconditioning. Circ Res. 1999; 84(9):973-9. DOI: 10.1161/01.res.84.9.973. View

Dickson E, Hogrefe C, Ludwig P, Ackermann L, Stoll L, Denning G . Exercise enhances myocardial ischemic tolerance via an opioid receptor-dependent mechanism. Am J Physiol Heart Circ Physiol. 2007; 294(1):H402-8. DOI: 10.1152/ajpheart.00280.2007. View

Parra V, Macho P, Domenech R . Late cardiac preconditioning by exercise in dogs is mediated by mitochondrial potassium channels. J Cardiovasc Pharmacol. 2010; 56(3):268-74. DOI: 10.1097/FJC.0b013e3181eb3049. View

10.

Kang M, Walker J . Protein kinase C delta and epsilon mediate positive inotropy in adult ventricular myocytes. J Mol Cell Cardiol. 2005; 38(5):753-64. DOI: 10.1016/j.yjmcc.2005.02.017. View

11.

Iemitsu M, Maeda S, Jesmin S, Otsuki T, Kasuya Y, Miyauchi T . Activation pattern of MAPK signaling in the hearts of trained and untrained rats following a single bout of exercise. J Appl Physiol (1985). 2006; 101(1):151-63. DOI: 10.1152/japplphysiol.00392.2005. View

12.

Kavazis A . Exercise preconditioning of the myocardium. Sports Med. 2009; 39(11):923-35. DOI: 10.2165/11317870-000000000-00000. View

13.

Braz J, Gregory K, Pathak A, Zhao W, Sahin B, Klevitsky R . PKC-alpha regulates cardiac contractility and propensity toward heart failure. Nat Med. 2004; 10(3):248-54. DOI: 10.1038/nm1000. View

14.

Brown D, Lynch J, Armstrong C, Caruso N, Ehlers L, Johnson M . Susceptibility of the heart to ischaemia-reperfusion injury and exercise-induced cardioprotection are sex-dependent in the rat. J Physiol. 2005; 564(Pt 2):619-30. PMC: 1464442. DOI: 10.1113/jphysiol.2004.081323. View

15.

Yamashita N, Baxter G, Yellon D . Exercise directly enhances myocardial tolerance to ischaemia-reperfusion injury in the rat through a protein kinase C mediated mechanism. Heart. 2001; 85(3):331-6. PMC: 1729658. DOI: 10.1136/heart.85.3.331. View

16.

Murry C, Jennings R, Reimer K . Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation. 1986; 74(5):1124-36. DOI: 10.1161/01.cir.74.5.1124. View

17.

Lennon S, Quindry J, French J, Kim S, Mehta J, Powers S . Exercise and myocardial tolerance to ischaemia-reperfusion. Acta Physiol Scand. 2004; 182(2):161-9. DOI: 10.1111/j.1365-201X.2004.01346.x. View

18.

Tilley D, Rockman H . Role of beta-adrenergic receptor signaling and desensitization in heart failure: new concepts and prospects for treatment. Expert Rev Cardiovasc Ther. 2006; 4(3):417-32. DOI: 10.1586/14779072.4.3.417. View

19.

Young L, Balin B, Weis M . Gö 6983: a fast acting protein kinase C inhibitor that attenuates myocardial ischemia/reperfusion injury. Cardiovasc Drug Rev. 2005; 23(3):255-72. DOI: 10.1111/j.1527-3466.2005.tb00170.x. View

20.

Newton A . Protein kinase C: structure, function, and regulation. J Biol Chem. 1995; 270(48):28495-8. DOI: 10.1074/jbc.270.48.28495. View