CMTM8 Inhibits the Carcinogenesis and Progression of Bladder Cancer

Overview

Journal Oncol Rep

Specialty Oncology

Date 2015 Oct 28

PMID 26503336

Citations 16

Authors

Denghui Gao

Hao Hu

Ying Wang

Weidong Yu

Jianhua Zhou

Xiaofeng Wang

Weiping Wang

Chunyan Zhou

Kexin Xu

Affiliations

Soon will be listed here.

Abstract

Bladder cancer is the most common tumor of the urinary tract. The incidence of bladder cancer has increased in the last few decades, thus novel molecular markers for early diagnosis and more efficacious treatment are urgently needed. Chemokine‑like factor (CKLF)‑like MARVEL transmembrane domain containing 8 (CMTM8) is downregulated in several types of cancers and is associated with tumor progression. However, CMTM8 expression has been unexplored in bladder cancer to date. Our results revealed that the expression of CMTM8 was negative in 46 of 74 (62.2%) bladder cancer samples via immunohistochemistry assay. CMTM8 downregulation was associated with advancing tumor stage and tumor grade. CMTM8 was successfully overexpressed by lentivirus in EJ and T24 cells, and the CCK‑8 and Transwell assays showed that CMTM8 overexpression decreased cell proliferation, migration and invasion in vitro. In tumor xenografts upregulation of CMTM8 inhibited tumor growth and lymph node metastasis in vivo. In conclusion, overexpression of CMTM8 in bladder cancer results in reduced malignant cell growth, migration and invasion, which could make it a potential therapeutic target in the treatment of bladder cancer.

Citing Articles

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References

Mellon K, Wright C, Kelly P, Horne C, Neal D . Long-term outcome related to epidermal growth factor receptor status in bladder cancer. J Urol. 1995; 153(3 Pt 2):919-25. View

Crew J, OBrien T, Bradburn M, Fuggle S, Bicknell R, Cranston D . Vascular endothelial growth factor is a predictor of relapse and stage progression in superficial bladder cancer. Cancer Res. 1997; 57(23):5281-5. View

Neal D, Marsh C, Bennett M, Abel P, Hall R, Sainsbury J . Epidermal-growth-factor receptors in human bladder cancer: comparison of invasive and superficial tumours. Lancet. 1985; 1(8425):366-8. DOI: 10.1016/s0140-6736(85)91386-8. View

Li D, Jin C, Yin C, Zhang Y, Pang B, Tian L . An alternative splice form of CMTM8 induces apoptosis. Int J Biochem Cell Biol. 2007; 39(11):2107-19. DOI: 10.1016/j.biocel.2007.06.002. View

Burger M, Catto J, Dalbagni G, Grossman H, Herr H, Karakiewicz P . Epidemiology and risk factors of urothelial bladder cancer. Eur Urol. 2012; 63(2):234-41. DOI: 10.1016/j.eururo.2012.07.033. View

Wang L, Wu C, Zheng Y, Qiu X, Wang L, Fan H . Molecular cloning and characterization of chemokine-like factor super family member 1 (CKLFSF1), a novel human gene with at least 23 alternative splicing isoforms in testis tissue. Int J Biochem Cell Biol. 2004; 36(8):1492-501. DOI: 10.1016/j.biocel.2003.11.017. View

Salvi A, Arici B, Portolani N, Giulini S, de Petro G, Barlati S . In vitro c-met inhibition by antisense RNA and plasmid-based RNAi down-modulates migration and invasion of hepatocellular carcinoma cells. Int J Oncol. 2007; 31(2):451-60. View

Jin C, Ding P, Wang Y, Ma D . Regulation of EGF receptor signaling by the MARVEL domain-containing protein CKLFSF8. FEBS Lett. 2005; 579(28):6375-82. DOI: 10.1016/j.febslet.2005.10.021. View

Polyak K, Weinberg R . Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits. Nat Rev Cancer. 2009; 9(4):265-73. DOI: 10.1038/nrc2620. View

10.

Fu L, Liu N, Han Y, Xie C, Li Q, Wang E . ADAM10 regulates proliferation, invasion, and chemoresistance of bladder cancer cells. Tumour Biol. 2014; 35(9):9263-8. DOI: 10.1007/s13277-014-2201-9. View

11.

Chen S, Du Y, Huang Y, Wang X, Gu Q, Dong Z . [Effects of novel human chemokine-like factor superfamily 8 on proliferation and EGFR expression of HL-60 cells]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2007; 15(3):458-61. View

12.

Babjuk M, Burger M, Zigeuner R, Shariat S, van Rhijn B, Comperat E . EAU guidelines on non-muscle-invasive urothelial carcinoma of the bladder: update 2013. Eur Urol. 2013; 64(4):639-53. DOI: 10.1016/j.eururo.2013.06.003. View

13.

Both J, Krijgsman O, Bras J, Schaap G, Baas F, Ylstra B . Focal chromosomal copy number aberrations identify CMTM8 and GPR177 as new candidate driver genes in osteosarcoma. PLoS One. 2015; 9(12):e115835. PMC: 4281204. DOI: 10.1371/journal.pone.0115835. View

14.

Chow N, Liu H, Lee E, Chang C, Chan S, Cheng H . Significance of urinary epidermal growth factor and its receptor expression in human bladder cancer. Anticancer Res. 1997; 17(2B):1293-6. View

15.

Choi Y, Woo S, Cho S, Moon H, Yun Y, Kim J . Brain-metastatic triple-negative breast cancer cells regain growth ability by altering gene expression patterns. Cancer Genomics Proteomics. 2013; 10(6):265-75. View

16.

Han W, Lou Y, Zhang Y, Chen Y, Li Y, Gu W . Molecular cloning and characterization of chemokine-like factor 1 (CKLF1), a novel human cytokine with unique structure and potential chemotactic activity. Biochem J. 2001; 357(Pt 1):127-35. PMC: 1221935. DOI: 10.1042/0264-6021:3570127. View

17.

Guillaume L, Guy L . [Epidemiology of and risk factors for bladder cancer and for urothelial tumors]. Rev Prat. 2015; 64(10):1372-4, 1378-80. View

18.

Jin C, Wang Y, Han W, Zhang Y, He Q, Li D . CMTM8 induces caspase-dependent and -independent apoptosis through a mitochondria-mediated pathway. J Cell Physiol. 2006; 211(1):112-20. DOI: 10.1002/jcp.20914. View

19.

Torre L, Bray F, Siegel R, Ferlay J, Lortet-Tieulent J, Jemal A . Global cancer statistics, 2012. CA Cancer J Clin. 2015; 65(2):87-108. DOI: 10.3322/caac.21262. View

20.

Kumagai T, Takeyama N, Yabuki T, Harada M, Miki Y, Kanou H . Apheresis of activated leukocytes with an immobilized polymyxin B filter in patients with septic shock. Shock. 2010; 34(5):461-6. DOI: 10.1097/SHK.0b013e3181e14ca0. View