ADAM10 Regulates Proliferation, Invasion, and Chemoresistance of Bladder Cancer Cells

Overview

Journal Tumour Biol

Publisher Sage Publications

Specialty Oncology

Date 2014 Jun 18

PMID 24935471

Citations 18

Authors

Lin Fu

Nan Liu

Yong Han

ChengYao Xie

Qingchang Li

Enhua Wang

Affiliations

Soon will be listed here.

Abstract

A disintegrin and metalloprotease 10 (ADAM10) is upregulated in several cancers and associates with malignant cancer progression. However, its expression pattern in bladder cancer remains unexplored. In the present study, we examined ADAM10 expression in 105 bladder cancer specimens using immunohistochemistry. We found ADAM10 overexpression in 51 of 105 (48.5 %) bladder cancer specimens. ADAM10 overexpression associated with advanced tumor stage (p = 0.001) and tumor grade (p = 0.018). To explore its biological functions in bladder cancer cells, small interfering RNA (siRNA) knockdown was performed in 5,637 and T24 cell lines. Cell Counting Kit-8 (CCK8) assay and Matrigel invasion assay showed that ADAM10 depletion decreased cell proliferation, migration, and invasion. In addition, ADAM10 knockdown increased the level of cisplatin-induced apoptosis. In conclusion, ADAM10 is overexpressed in bladder cancer and regulates malignant cell growth and invasion, which makes it a candidate therapeutic target.

Citing Articles

GW501516-Mediated Targeting of Tetraspanin 15 Regulates ADAM10-Dependent N-Cadherin Cleavage in Invasive Bladder Cancer Cells.

Barbaud A, Lascombe I, Pechery A, Arslan S, Kleinclauss F, Fauconnet S Cells. 2024; 13(8.

PMID: 38667323 PMC: 11049359. DOI: 10.3390/cells13080708.

Evaluation of Cisplatin-Induced Acute Renal Failure Amelioration Using Fondaparinux and Alteplase.

Abdel-Bakky M, Aldakhili A, Ali H, Babiker A, Alhowail A, Mohammed S Pharmaceuticals (Basel). 2023; 16(7).

PMID: 37513824 PMC: 10383028. DOI: 10.3390/ph16070910.

Small extracellular vesicles promote invadopodia activity in glioblastoma cells in a therapy-dependent manner.

Whitehead C, Fang H, Su H, Morokoff A, Kaye A, Hanssen E Cell Oncol (Dordr). 2023; 46(4):909-931.

PMID: 37014551 PMC: 10356899. DOI: 10.1007/s13402-023-00786-w.

Expression of ADAM Proteases in Bladder Cancer Patients with BCG Failure: A Pilot Study.

Pichler R, Lindner A, Schafer G, Tulchiner G, Staudacher N, Mayr M J Clin Med. 2021; 10(4).

PMID: 33672843 PMC: 7917772. DOI: 10.3390/jcm10040764.

The microRNA miR-3174 Suppresses the Expression of ADAM15 and Inhibits the Proliferation of Patient-Derived Bladder Cancer Cells.

Yu C, Wang Y, Liu T, Sha K, Song Z, Zhao M Onco Targets Ther. 2020; 13:4157-4168.

PMID: 32547057 PMC: 7244357. DOI: 10.2147/OTT.S246710.

References

Edwards D, Handsley M, Pennington C . The ADAM metalloproteinases. Mol Aspects Med. 2008; 29(5):258-89. PMC: 7112278. DOI: 10.1016/j.mam.2008.08.001. View

Duffy M, McKiernan E, ODonovan N, McGowan P . Role of ADAMs in cancer formation and progression. Clin Cancer Res. 2009; 15(4):1140-4. DOI: 10.1158/1078-0432.CCR-08-1585. View

DAmelio M, Cavallucci V, Cecconi F . Neuronal caspase-3 signaling: not only cell death. Cell Death Differ. 2009; 17(7):1104-14. DOI: 10.1038/cdd.2009.180. View

Yang C, Jiang F, Xu F, Jiang G . ADAM10 overexpression confers resistance to doxorubicin-induced apoptosis in hepatocellular carcinoma. Tumour Biol. 2012; 33(5):1535-41. DOI: 10.1007/s13277-012-0405-4. View

Siegel R, Naishadham D, Jemal A . Cancer statistics, 2012. CA Cancer J Clin. 2012; 62(1):10-29. DOI: 10.3322/caac.20138. View

Wang C, Wang W, Wang J, Zhan H, Jiang L, Yan R . Apoptin induces apoptosis in nude mice allograft model of human bladder cancer by altering multiple bladder tumor-associated gene expression profiles. Tumour Biol. 2013; 34(3):1667-78. DOI: 10.1007/s13277-013-0700-8. View

Yan L, Yan K, Kun W, Xu L, Ma Q, Tang Y . Berberine inhibits the migration and invasion of T24 bladder cancer cells via reducing the expression of heparanase. Tumour Biol. 2012; 34(1):215-21. DOI: 10.1007/s13277-012-0531-z. View

Yan F, Liu Y, Wang W . Matrine inhibited the growth of rat osteosarcoma UMR-108 cells by inducing apoptosis in a mitochondrial-caspase-dependent pathway. Tumour Biol. 2013; 34(4):2135-40. DOI: 10.1007/s13277-013-0744-9. View

Lendeckel U, Kohl J, Arndt M, Carl-McGrath S, Donat H, Rocken C . Increased expression of ADAM family members in human breast cancer and breast cancer cell lines. J Cancer Res Clin Oncol. 2004; 131(1):41-8. DOI: 10.1007/s00432-004-0619-y. View

10.

Saftig P, Reiss K . The "A Disintegrin And Metalloproteases" ADAM10 and ADAM17: novel drug targets with therapeutic potential?. Eur J Cell Biol. 2011; 90(6-7):527-35. DOI: 10.1016/j.ejcb.2010.11.005. View

11.

Han B, Luan L, Xu Z, Wu B . Expression and biological roles of SATB1 in human bladder cancer. Tumour Biol. 2013; 34(5):2943-9. DOI: 10.1007/s13277-013-0857-1. View

12.

Lee S, Schramme A, Doberstein K, Dummer R, Abdel-Bakky M, Keller S . ADAM10 is upregulated in melanoma metastasis compared with primary melanoma. J Invest Dermatol. 2009; 130(3):763-73. DOI: 10.1038/jid.2009.335. View

13.

Sanchez-Carbayo M . Hypermethylation in bladder cancer: biological pathways and translational applications. Tumour Biol. 2012; 33(2):347-61. DOI: 10.1007/s13277-011-0310-2. View

14.

Rocks N, Paulissen G, El Hour M, Quesada F, Crahay C, Gueders M . Emerging roles of ADAM and ADAMTS metalloproteinases in cancer. Biochimie. 2007; 90(2):369-79. DOI: 10.1016/j.biochi.2007.08.008. View

15.

Xu Q, Liu X, Chen W, Zhang Z . Inhibiting adenoid cystic carcinoma cells growth and metastasis by blocking the expression of ADAM 10 using RNA interference. J Transl Med. 2010; 8:136. PMC: 3017514. DOI: 10.1186/1479-5876-8-136. View

16.

Gavert N, Conacci-Sorrell M, Gast D, Schneider A, Altevogt P, Brabletz T . L1, a novel target of beta-catenin signaling, transforms cells and is expressed at the invasive front of colon cancers. J Cell Biol. 2005; 168(4):633-42. PMC: 2171754. DOI: 10.1083/jcb.200408051. View

17.

Ko S, Lin S, Wong Y, Liu C, Chang K, Liu T . Increase of disintergin metalloprotease 10 (ADAM10) expression in oral squamous cell carcinoma. Cancer Lett. 2005; 245(1-2):33-43. DOI: 10.1016/j.canlet.2005.10.019. View

18.

Guo J, He L, Yuan P, Wang P, Lu Y, Tong F . ADAM10 overexpression in human non-small cell lung cancer correlates with cell migration and invasion through the activation of the Notch1 signaling pathway. Oncol Rep. 2012; 28(5):1709-18. DOI: 10.3892/or.2012.2003. View

19.

Gaida M, Haag N, Gunther F, Tschaharganeh D, Schirmacher P, Friess H . Expression of A disintegrin and metalloprotease 10 in pancreatic carcinoma. Int J Mol Med. 2010; 26(2):281-8. DOI: 10.3892/ijmm_00000463. View