Autoregulation of Topoisomerase I Expression by Supercoiling Sensitive Transcription

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2015 Oct 27

PMID 26496944

Citations 19

Authors

Wareed Ahmed

Shruti Menon

Pullela V D N B Karthik

Valakunja Nagaraja

Affiliations

Soon will be listed here.

Abstract

The opposing catalytic activities of topoisomerase I (TopoI/relaxase) and DNA gyrase (supercoiling enzyme) ensure homeostatic maintenance of bacterial chromosome supercoiling. Earlier studies in Escherichia coli suggested that the alteration in DNA supercoiling affects the DNA gyrase and TopoI expression. Although, the role of DNA elements around the promoters were proposed in regulation of gyrase, the molecular mechanism of supercoiling mediated control of TopoI expression is not yet understood. Here, we describe the regulation of TopoI expression from Mycobacterium tuberculosis and Mycobacterium smegmatis by a mechanism termed Supercoiling Sensitive Transcription (SST). In both the organisms, topoI promoter(s) exhibited reduced activity in response to chromosome relaxation suggesting that SST is intrinsic to topoI promoter(s). We elucidate the role of promoter architecture and high transcriptional activity of upstream genes in topoI regulation. Analysis of the promoter(s) revealed the presence of sub-optimal spacing between the -35 and -10 elements, rendering them supercoiling sensitive. Accordingly, upon chromosome relaxation, RNA polymerase occupancy was decreased on the topoI promoter region implicating the role of DNA topology in SST of topoI. We propose that negative supercoiling induced DNA twisting/writhing align the -35 and -10 elements to facilitate the optimal transcription of topoI.

Citing Articles

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Manipulation of topoisomerase expression inhibits cell division but not growth and reveals a distinctive promoter structure in Synechocystis.

Behle A, Dietsch M, Goldschmidt L, Murugathas W, Berwanger L, Burmester J Nucleic Acids Res. 2022; 50(22):12790-12808.

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RNase HI Depletion Strongly Potentiates Cell Killing by Rifampicin in Mycobacteria.

Al-Zubaidi A, Cheung C, Cook G, Taiaroa G, Mizrahi V, Lott J Antimicrob Agents Chemother. 2022; 66(10):e0209121.

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The regulation of DNA supercoiling across evolution.

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Combining transposon mutagenesis and reporter genes to identify novel regulators of the topA promoter in Streptomyces.

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