Cases of Echinococcus Granulosus Sensu Stricto Isolated from Polish Patients: Imported or Indigenous?

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2015 Oct 23

PMID 26491683

Citations 6

Authors

Monika Dybicz

Piotr Karol Borkowski

Julia Dabrowska

Lidia Chomicz

Affiliations

Soon will be listed here.

Abstract

The cases of nine Polish patients with diagnosed cystic echinococcosis (CE) were examined. A total of nine isolates obtained postoperatively were investigated using PCR and sequencing. The mitochondrial region of nad1 gene was amplified. This PCR and sequencing analysis revealed the presence of Echinococcus canadensis G7 in seven patients and E. granulosus G1 in two patients. These data demonstrate that E. canadensis is the predominant causative agent of human cystic echinococcosis in Poland. E. granulosus G1 detection in Polish patients suggests that the parasite was imported; however it does not exclude the possibility that these cases could have been of Polish origin.

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References

Dybicz M, Gierczak A, Dabrowska J, Rdzanek L, Michalowicz B . Molecular diagnosis of cystic echinococcosis in humans from central Poland. Parasitol Int. 2013; 62(4):364-7. DOI: 10.1016/j.parint.2013.03.005. View

Aaty H, Abdel-Hameed D, Alam-Eldin Y, El-Shennawy S, Aminou H, Makled S . Molecular genotyping of Echinococcus granulosus in animal and human isolates from Egypt. Acta Trop. 2011; 121(2):125-8. DOI: 10.1016/j.actatropica.2011.10.014. View

Konyaev S, Yanagida T, Nakao M, Ingovatova G, Shoykhet Y, Bondarev A . Genetic diversity of Echinococcus spp. in Russia. Parasitology. 2013; 140(13):1637-47. DOI: 10.1017/S0031182013001340. View

Snabel V, DAmelio S, Mathiopoulos K, Turcekova L, Dubinsky P . Molecular evidence for the presence of a G7 genotype of Echinococcus granulosus in Slovakia. J Helminthol. 2000; 74(2):177-81. View

Seimenis A . Overview of the epidemiological situation on echinococcosis in the Mediterranean region. Acta Trop. 2003; 85(2):191-5. DOI: 10.1016/s0001-706x(02)00272-3. View

Turcekova L, Snabel V, DAmelio S, Busi M, Dubinsky P . Morphological and genetic characterization of Echinococcus granulosus in the Slovak Republic. Acta Trop. 2003; 85(2):223-9. DOI: 10.1016/s0001-706x(02)00229-2. View

Gonzalez L, Daniel-Mwambete K, Montero E, Rosenzvit M, McManus D, Garate T . Further molecular discrimination of Spanish strains of Echinococcus granulosus. Exp Parasitol. 2003; 102(1):46-56. DOI: 10.1016/s0014-4894(02)00146-7. View

Ito A, Urbani C, Jiamin Q, Vuitton D, Dongchuan Q, Heath D . Control of echinococcosis and cysticercosis: a public health challenge to international cooperation in China. Acta Trop. 2003; 86(1):3-17. DOI: 10.1016/s0001-706x(02)00269-3. View

Pawlowski Z, Stefaniak J . The pig strain of Echinococcus granulosus in humans: a neglected issue?. Trends Parasitol. 2003; 19(10):439. DOI: 10.1016/s1471-4922(03)00199-5. View

10.

Guarnera E, Parra A, Kamenetzky L, Garcia G, Gutierrez A . Cystic echinococcosis in Argentina: evolution of metacestode and clinical expression in various Echinococcus granulosus strains. Acta Trop. 2004; 92(2):153-9. DOI: 10.1016/j.actatropica.2004.06.008. View

11.

Bowles J, McManus D . NADH dehydrogenase 1 gene sequences compared for species and strains of the genus Echinococcus. Int J Parasitol. 1993; 23(7):969-72. DOI: 10.1016/0020-7519(93)90065-7. View

12.

Alvarez Rojas C, Romig T, Lightowlers M . Echinococcus granulosus sensu lato genotypes infecting humans--review of current knowledge. Int J Parasitol. 2013; 44(1):9-18. DOI: 10.1016/j.ijpara.2013.08.008. View

13.

Monteiro D, Botton S, Tonin A, Azevedo M, Graichen D, Noal C . Echinococcus canadensis (G7) and Echinococcus granulosus sensu stricto (G1) in swine of southern Brazil. Vet Parasitol. 2014; 202(3-4):335-8. DOI: 10.1016/j.vetpar.2014.01.023. View

14.

Ito A, Dorjsuren T, Davaasuren A, Yanagida T, Sako Y, Nakaya K . Cystic echinococcoses in Mongolia: molecular identification, serology and risk factors. PLoS Negl Trop Dis. 2014; 8(6):e2937. PMC: 4063745. DOI: 10.1371/journal.pntd.0002937. View

15.

Zhang T, Yang D, Zeng Z, Zhao W, Liu A, Piao D . Genetic characterization of human-derived hydatid cysts of Echinococcus granulosus sensu lato in Heilongjiang Province and the first report of G7 genotype of E. canadensis in humans in China. PLoS One. 2014; 9(10):e109059. PMC: 4199617. DOI: 10.1371/journal.pone.0109059. View

16.

Jenkins D, Romig T, Thompson R . Emergence/re-emergence of Echinococcus spp.--a global update. Int J Parasitol. 2005; 35(11-12):1205-19. DOI: 10.1016/j.ijpara.2005.07.014. View

17.

Scott J, Stefaniak J, Pawlowski Z, McManus D . Molecular genetic analysis of human cystic hydatid cases from Poland: identification of a new genotypic group (G9) of Echinococcus granulosus. Parasitology. 1997; 114 ( Pt 1):37-43. DOI: 10.1017/s0031182096008062. View

18.

Rodriguez-Prado U, Jimenez-Gonzalez D, Avila G, Gonzalez A, Martinez-Flores W, Mondragon de la Pena C . Short report: Genetic variation of Echinococcus canadensis (G7) in Mexico. Am J Trop Med Hyg. 2014; 91(6):1149-53. PMC: 4257637. DOI: 10.4269/ajtmh.14-0317. View

19.

Bart J, Morariu S, Knapp J, Ilie M, Pitulescu M, Anghel A . Genetic typing of Echinococcus granulosus in Romania. Parasitol Res. 2005; 98(2):130-7. DOI: 10.1007/s00436-005-0015-9. View

20.

Varcasia A, Canu S, Lightowlers M, Scala A, Garippa G . Molecular characterization of Echinococcus granulosus strains in Sardinia. Parasitol Res. 2005; 98(3):273-7. DOI: 10.1007/s00436-005-0059-x. View