Determination of Angiotensin-(1-7) with HPLC/Fluorescence-Detection

Overview

Journal J Fluoresc

Specialties Biophysics
Chemistry

Date 2015 Oct 11

PMID 26452351

Citations 1

Authors

Miriam Russ

Susanne Hauser

Reinhold Wintersteiger

Joachim Greilberger

Michaela Andra

Astrid Ortner

Affiliations

Soon will be listed here.

Abstract

Angiotensin-(1-7) is an important active component in the renin-angiotensin-system. Due to its cardio protective effects it is now under investigation in combination with antioxidants as a reperfusion solution. The combination showed impressive effects on isolated hearts of male Wistar rats after induced ischemia. In this work a high performance liquid chromatography method with fluorescence detection was developed for the first time for in-process measurements as well as for stability tests of the peptide in the novel antioxidant-containing Karal® solution. For fluorescence detection of angiotensin-(1-7) fluorescamine as derivatization dye was applied. Under optimized conditions the method showed linearity over the range of 50 to 5000 ng/mL with R(2) of 0.9988 and an overall precision better than 5.0 %. LOD and LOQ were determined to be in the femtomol range on column. It was found that stability of angiotensin-(1-7) could be significantly improved in the antioxidant containing preparation compared to aqueous solutions.

Citing Articles

Angiotensin-(1-7) Participates in Enhanced Skeletal Muscle Insulin Sensitivity After a Bout of Exercise.

Echeverria-Rodriguez O, Gallardo-Ortiz I, Del Valle-Mondragon L, Villalobos-Molina R J Endocr Soc. 2020; 4(2):bvaa007.

PMID: 32104748 PMC: 7039407. DOI: 10.1210/jendso/bvaa007.

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