Genetic Relationships Among Escherichia Coli Isolates Causing Urinary Tract Infections in Humans and Animals

Overview

Journal Epidemiol Infect

Publisher Cambridge University Press

Specialties Infectious Diseases
Public Health

Date 1989 Feb 1

PMID 2645153

Citations 21

Authors

T S Whittam

M L Wolfe

R A Wilson

Affiliations

Soon will be listed here.

Abstract

Genetic variation in isolates of Escherichia coli obtained mostly from urinary tract infections in humans and domesticated animals (dogs and cats) was assessed for 16 enzymes using multilocus enzyme electrophoresis to characterize chromosomal genotypes. A total of 148 isolates comprised 63 distinct electrophoretic types (ETs) and about half of the isolates belonged to one of 9 common ETs. A bootstrap analysis of genetic distance between ETs revealed three significant groups of strains. Variation in allele frequencies among groups accounted for 40% of the total genetic diversity. The majority of the common ETs fell into a major cluster of closely related strains. The recovery of multiple isolates of the same electrophoretic types and serotypes from unassociated hosts suggests that these bacteria represent uropathogenic clones that are widely disseminated in humans and animals.

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References

ORSKOV I, ORSKOV F, BIRCH-ANDERSEN A, Kanamori M . O, K, H and fimbrial antigens in Escherichia coli serotypes associated with pyelonephritis and cystitis. Scand J Infect Dis Suppl. 1982; 33:18-25. View

Whittam T, Wilson R . Genetic relationships among pathogenic Escherichia coli of serogroup O157. Infect Immun. 1988; 56(9):2467-73. PMC: 259589. DOI: 10.1128/iai.56.9.2467-2473.1988. View

Caugant D, Levin B, Whittam T, Svanborg Eden C, Selander R . Genetic diversity and relationships among strains of Escherichia coli in the intestine and those causing urinary tract infections. Prog Allergy. 1983; 33:203-27. DOI: 10.1159/000318331. View

Hughes C, Hacker J, Roberts A, Goebel W . Hemolysin production as a virulence marker in symptomatic and asymptomatic urinary tract infections caused by Escherichia coli. Infect Immun. 1983; 39(2):546-51. PMC: 347985. DOI: 10.1128/iai.39.2.546-551.1983. View

Whittam T, Ochman H, Selander R . Multilocus genetic structure in natural populations of Escherichia coli. Proc Natl Acad Sci U S A. 1983; 80(6):1751-5. PMC: 393682. DOI: 10.1073/pnas.80.6.1751. View

Elo J, Vaisanen E, Siitonen A, ORSKOV I, ORSKOV F, Svenson S . P-fimbriated clones among uropathogenic Escherichia coli strains. Infect Immun. 1984; 43(1):149-55. PMC: 263402. DOI: 10.1128/iai.43.1.149-155.1984. View

Stenderup J, ORSKOV F . The clonal nature of enteropathogenic Escherichia coli strains. J Infect Dis. 1983; 148(6):1019-24. DOI: 10.1093/infdis/148.6.1019. View

Ochman H, Selander R . Evidence for clonal population structure in Escherichia coli. Proc Natl Acad Sci U S A. 1984; 81(1):198-201. PMC: 344638. DOI: 10.1073/pnas.81.1.198. View

Achtman M, Heuzenroeder M, Kusecek B, Ochman H, Caugant D, Selander R . Clonal analysis of Escherichia coli O2:K1 isolated from diseased humans and animals. Infect Immun. 1986; 51(1):268-76. PMC: 261097. DOI: 10.1128/iai.51.1.268-276.1986. View

10.

Selander R, Korhonen T, Williams P, Pattison P, Caugant D . Genetic relationships and clonal structure of strains of Escherichia coli causing neonatal septicemia and meningitis. Infect Immun. 1986; 52(1):213-22. PMC: 262222. DOI: 10.1128/iai.52.1.213-222.1986. View

11.

Selander R, Caugant D, Ochman H, Musser J, Gilmour M, Whittam T . Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986; 51(5):873-84. PMC: 238981. DOI: 10.1128/aem.51.5.873-884.1986. View

12.

Gruneberg R, Bettelheim K . Geographical variation in serological types of urinary Escherichia coli. J Med Microbiol. 1969; 2(3):219-24. DOI: 10.1099/00222615-2-3-219. View

13.

Mabeck C, ORSKOV F, ORSKOV I . Escherichia coli serotypes and renal involvement in urinary-tract infection. Lancet. 1971; 1(7713):1312-4. DOI: 10.1016/s0140-6736(71)91884-8. View

14.

Cooke E, Ewins S . Properties of strains of Escherichia coli isolated from a variety of sources. J Med Microbiol. 1975; 8(1):107-11. DOI: 10.1099/00222615-8-1-107. View

15.

Roberts A, Linton J, Waterman A, Gower P, Koutsaimanis K . Urinary and faecal Escherichia coli O-sero-groups in symptomatic urinary-tract infection and asymptomatic bacteriuria. J Med Microbiol. 1975; 8(2):311-8. DOI: 10.1099/00222615-8-2-311. View

16.

Kaijser B, Hanson L, Jodal U, Robbins J . Frequency of E. coli K antigens in urinary-tract infections in children. Lancet. 1977; 1(8013):663-6. DOI: 10.1016/s0140-6736(77)92111-0. View

17.

ORSKOV I, ORSKOV F, JANN B, Jann K . Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977; 41(3):667-710. PMC: 414020. DOI: 10.1128/br.41.3.667-710.1977. View

18.

Belsheim J, Gnarpe H, Kaijser B . Chemoattractive factors from Escherichia coli: individual strain variation. Infect Immun. 1981; 31(1):7-10. PMC: 351744. DOI: 10.1128/iai.31.1.7-10.1981. View

19.

Kallenius G, Mollby R, Svenson S, Helin I, Hultberg H, Cedergren B . Occurrence of P-fimbriated Escherichia coli in urinary tract infections. Lancet. 1981; 2(8260-61):1369-72. DOI: 10.1016/s0140-6736(81)92797-5. View

20.

Achtman M, Pluschke G . Clonal analysis of descent and virulence among selected Escherichia coli. Annu Rev Microbiol. 1986; 40:185-210. DOI: 10.1146/annurev.mi.40.100186.001153. View