Validation of a RP-HPLC-DAD Method for Chamomile (Matricaria Recutita) Preparations and Assessment of the Marker, Apigenin-7-glucoside, Safety and Anti-Inflammatory Effect

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2015 Oct 1

PMID 26421053

Citations 17

Authors

Felipe Galeti Miguel

Amanda Henriques Cavalheiro

Nathalia Favaretto Spinola

Diego Luis Ribeiro

Gustavo Rafael Mazzaron Barcelos

Lusania Maria Greggi Antunes

Juliana Issa Hori

Franciane Marquele-Oliveira

Bruno Alves Rocha

Andresa Aparecida Berretta

Affiliations

Soon will be listed here.

Abstract

Chamomile is a medicinal plant, which presents several biological effects, especially the anti-inflammatory effect. One of the compounds related to this effect is apigenin, a flavonoid that is mostly found in its glycosylated form, apigenin-7-glucoside (APG), in natural sources. However, the affectivity and safety of this glycoside have not been well explored for topical application. In this context, the aim of this work was to develop and validate a reversed-phase high-performance liquid chromatography (RP-HPLC-DAD) method to quantify APG in chamomile preparations. Additionally, the safety and the anti-inflammatory potential of this flavonoid were verified. The RP-HPLC-DAD method was developed and validated with linearity at 24.0-36.0 μg/mL range (r = 0.9994). Intra- and interday precision (RSD) were 0.27-2.66% and accuracy was 98.27-101.21%. The validated method was applied in the analysis of chamomile flower heads, glycolic extract, and Kamillen cream, supporting the method application in the quality control of chamomile preparations. Furthermore, the APG safety was assessed by MTT cytotoxicity assay and mutagenic protocols and the anti-inflammatory activity was confirmed by a diminished TNF-α production showed by mice macrophages treated with APG following LPS treatment.

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References

Srivastava J, Shankar E, Gupta S . Chamomile: A herbal medicine of the past with bright future. Mol Med Rep. 2010; 3(6):895-901. PMC: 2995283. DOI: 10.3892/mmr.2010.377. View

McKay D, Blumberg J . A review of the bioactivity and potential health benefits of chamomile tea (Matricaria recutita L.). Phytother Res. 2006; 20(7):519-30. DOI: 10.1002/ptr.1900. View

Krol W, Shani J, Czuba Z, Scheller S . Modulating luminol-dependent chemiluminescence of neutrophils by flavones. Z Naturforsch C J Biosci. 1992; 47(11-12):889-92. DOI: 10.1515/znc-1992-11-1216. View

Chao S, Huang S, Hu D, Lin H . Subtoxic Levels of Apigenin Inhibit Expression and Secretion of VEGF by Uveal Melanoma Cells via Suppression of ERK1/2 and PI3K/Akt Pathways. Evid Based Complement Alternat Med. 2013; 2013:817674. PMC: 3833119. DOI: 10.1155/2013/817674. View

Tomic M, Popovic V, Petrovic S, Stepanovic-Petrovic R, Micov A, Pavlovic-Drobac M . Antihyperalgesic and antiedematous activities of bisabolol-oxides-rich matricaria oil in a rat model of inflammation. Phytother Res. 2013; 28(5):759-66. DOI: 10.1002/ptr.5057. View

Jarrahi M . An experimental study of the effects of Matricaria chamomilla extract on cutaneous burn wound healing in albino rats. Nat Prod Res. 2008; 22(5):422-7. DOI: 10.1080/14786410701591713. View

Uhl M, Helma C, Knasmuller S . Single-cell gel electrophoresis assays with human-derived hepatoma (Hep G2) cells. Mutat Res. 1999; 441(2):215-24. DOI: 10.1016/s1383-5718(99)00050-9. View

Fonseca F, Tavares M . Validation of a capillary electrophoresis method for the quantitative determination of free and total apigenin in extracts of Chamomilla recutita. Phytochem Anal. 2004; 15(1):65-70. DOI: 10.1002/pca.744. View

Sebai H, Jabri M, Souli A, Rtibi K, Selmi S, Tebourbi O . Antidiarrheal and antioxidant activities of chamomile (Matricaria recutita L.) decoction extract in rats. J Ethnopharmacol. 2014; 152(2):327-32. DOI: 10.1016/j.jep.2014.01.015. View

10.

Guzelmeric E, Vovk I, Yesilada E . Development and validation of an HPTLC method for apigenin 7-O-glucoside in chamomile flowers and its application for fingerprint discrimination of chamomile-like materials. J Pharm Biomed Anal. 2015; 107:108-18. DOI: 10.1016/j.jpba.2014.12.021. View

11.

Man M, Hupe M, Sun R, Man G, Mauro T, Elias P . Topical apigenin alleviates cutaneous inflammation in murine models. Evid Based Complement Alternat Med. 2013; 2012:912028. PMC: 3524999. DOI: 10.1155/2012/912028. View

12.

Choi S, Jeong C, Cho S, Lee Y . Mechanism of apoptosis induced by apigenin in HepG2 human hepatoma cells: involvement of reactive oxygen species generated by NADPH oxidase. Arch Pharm Res. 2007; 30(10):1328-35. DOI: 10.1007/BF02980274. View

13.

Mosmann T . Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983; 65(1-2):55-63. DOI: 10.1016/0022-1759(83)90303-4. View

14.

Cemeli E, Baumgartner A, Anderson D . Antioxidants and the Comet assay. Mutat Res. 2008; 681(1):51-67. DOI: 10.1016/j.mrrev.2008.05.002. View

15.

Schreiber A, Carle R, Reinhard E . On the accumulation of apigenin in chamomile flowers. Planta Med. 1990; 56(2):179-81. DOI: 10.1055/s-2006-960920. View

16.

Guimaraes R, Barros L, Duenas M, Calhelha R, Carvalho A, Santos-Buelga C . Infusion and decoction of wild German chamomile: bioactivity and characterization of organic acids and phenolic compounds. Food Chem. 2012; 136(2):947-54. DOI: 10.1016/j.foodchem.2012.09.007. View

17.

Smolinski A, Pestka J . Modulation of lipopolysaccharide-induced proinflammatory cytokine production in vitro and in vivo by the herbal constituents apigenin (chamomile), ginsenoside Rb(1) (ginseng) and parthenolide (feverfew). Food Chem Toxicol. 2003; 41(10):1381-90. DOI: 10.1016/s0278-6915(03)00146-7. View

18.

Tice R, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H . Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000; 35(3):206-21. DOI: 10.1002/(sici)1098-2280(2000)35:3<206::aid-em8>3.0.co;2-j. View

19.

Griffiths L, Smith G . Metabolism of apigenin and related compounds in the rat. Metabolite formation in vivo and by the intestinal microflora in vitro. Biochem J. 1972; 128(4):901-11. PMC: 1173909. DOI: 10.1042/bj1280901. View

20.

Tubaro A, Zilli C, Redaelli C, Della Loggia R . Evaluation of antiinflammatory activity of a chamomile extract topical application. Planta Med. 1984; 50(4):359. View