Splicing Diversity Revealed by Reduced Spliceosomes in C. Merolae and Other Organisms

Overview

Journal RNA Biol

Specialty Molecular Biology

Date 2015 Sep 25

PMID 26400738

Citations 14

Authors

Andrew J Hudson

Martha R Stark

Naomi M Fast

Anthony G Russell

Stephen D Rader

Affiliations

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Abstract

Pre-mRNA splicing has been considered one of the hallmarks of eukaryotes, yet its diversity is astonishing: the number of substrate introns for splicing ranges from hundreds of thousands in humans to a mere handful in certain parasites. The catalytic machinery that carries out splicing, the spliceosome, is similarly diverse, with over 300 associated proteins in humans to a few tens in other organisms. In this Point of View, we discuss recent work characterizing the reduced spliceosome of the acidophilic red alga Cyanidioschyzon merolae, which further highlights the diversity of splicing in that it does not possess the U1 snRNP that is characteristically responsible for 5' splice site recognition. Comparisons to other organisms with reduced spliceosomes, such as microsporidia, trypanosomes, and Giardia, help to identify the most highly conserved splicing factors, pointing to the essential core of this complex machine. These observations argue for increased exploration of important biochemical processes through study of a wider ranger of organisms.

Citing Articles

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