Dynamic and Social Behaviors of Human Pluripotent Stem Cells

Overview

Journal Sci Rep

Specialty Science

Date 2015 Sep 19

PMID 26381699

Citations 11

Authors

Smruti M Phadnis

Nathan O Loewke

Ivan K Dimov

Sunil Pai

Christine E Amwake

Olav Solgaard

Thomas M Baer

Bertha Chen

Renee A Reijo Pera

Affiliations

Soon will be listed here.

Abstract

Human pluripotent stem cells (hPSCs) can self-renew or differentiate to diverse cell types, thus providing a platform for basic and clinical applications. However, pluripotent stem cell populations are heterogeneous and functional properties at the single cell level are poorly documented leading to inefficiencies in differentiation and concerns regarding reproducibility and safety. Here, we use non-invasive time-lapse imaging to continuously examine hPSC maintenance and differentiation and to predict cell viability and fate. We document dynamic behaviors and social interactions that prospectively distinguish hPSC survival, self-renewal, and differentiation. Results highlight the molecular role of E-cadherin not only for cell-cell contact but also for clonal propagation of hPSCs. Results indicate that use of continuous time-lapse imaging can distinguish cellular heterogeneity with respect to pluripotency as well as a subset of karyotypic abnormalities whose dynamic properties were monitored.

Citing Articles

A Cell-Based Optimised Approach for Rapid and Efficient Gene Editing of Human Pluripotent Stem Cells.

Cuevas-Ocana S, Yang J, Aushev M, Schlossmacher G, Bear C, Hannan N Int J Mol Sci. 2023; 24(12).

PMID: 37373413 PMC: 10299534. DOI: 10.3390/ijms241210266.

Cell Behavioral Dynamics as a Cue in Optimizing Culture Stabilization in the Bioprocessing of Pluripotent Stem Cells.

Thanuthanakhun N, Kim M, Kino-Oka M Bioengineering (Basel). 2022; 9(11).

PMID: 36354580 PMC: 9687444. DOI: 10.3390/bioengineering9110669.

Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics.

Lin S, Loza A, Antrim L, Talbot P Stem Cells Transl Med. 2021; 10(9):1343-1359.

PMID: 34089307 PMC: 8380446. DOI: 10.1002/sctm.15-0352.

Maintenance of hPSCs under Xeno-Free and Chemically Defined Culture Conditions.

Lim J, Kim H, Rhie B, Lee M, Choi M, Hong S Int J Stem Cells. 2019; 12(3):484-496.

PMID: 31658510 PMC: 6881038. DOI: 10.15283/ijsc19090.

An Efficient Protocol for Single-Cell Cloning Human Pluripotent Stem Cells.

Singh A Front Cell Dev Biol. 2019; 7:11.

PMID: 30766873 PMC: 6365467. DOI: 10.3389/fcell.2019.00011.

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