Metabolic Engineering of Escherichia Coli for Production of (2S,3S)-butane-2,3-diol from Glucose

Overview

Journal Biotechnol Biofuels

Publisher Biomed Central

Specialty Biotechnology

Date 2015 Sep 18

PMID 26379775

Citations 12

Authors

Haipei Chu

Bo Xin

Peihai Liu

Yu Wang

Lixiang Li

Xiuxiu Liu

Xuan Zhang

Cuiqing Ma

Ping Xu

Chao Gao

Affiliations

Soon will be listed here.

Abstract

Background: Butane-2,3-diol (2,3-BD) is a fuel and platform biochemical with various industrial applications. 2,3-BD exists in three stereoisomeric forms: (2R,3R)-2,3-BD, meso-2,3-BD and (2S,3S)-2,3-BD. Microbial fermentative processes have been reported for (2R,3R)-2,3-BD and meso-2,3-BD production.

Results: The production of (2S,3S)-2,3-BD from glucose was acquired by whole cells of recombinant Escherichia coli coexpressing the α-acetolactate synthase and meso-butane-2,3-diol dehydrogenase of Enterobacter cloacae subsp. dissolvens strain SDM. An optimal biocatalyst for (2S,3S)-2,3-BD production, E. coli BL21 (pETDuet-PT7-budB-PT7-budC), was constructed and the bioconversion conditions were optimized. With the addition of 10 mM FeCl3 in the bioconversion system, (2S,3S)-2,3-BD at a concentration of 2.2 g/L was obtained with a stereoisomeric purity of 95.0 % using the metabolically engineered strain from glucose.

Conclusions: The engineered E. coli strain is the first one that can be used in the direct production of (2S,3S)-2,3-BD from glucose. The results demonstrated that the method developed here would be a promising process for efficient (2S,3S)-2,3-BD production.

Citing Articles

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Metabolic Engineering and Regulation of Diol Biosynthesis from Renewable Biomass in .

Wu T, Liu Y, Liu J, Chen Z, Huo Y Biomolecules. 2022; 12(5).

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Dehydrogenation Mechanism of Three Stereoisomers of Butane-2,3-Diol in KT2440.

Liu Y, Wang X, Ma L, Lu M, Zhang W, Lu C Front Bioeng Biotechnol. 2021; 9:728767.

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Increasing ATP turnover boosts productivity of 2,3-butanediol synthesis in Escherichia coli.

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