Relationships Between Genetic Diversity and Fusarium Toxin Profiles of Winter Wheat Cultivars

Overview

Journal Plant Pathol J

Date 2015 Sep 12

PMID 26361471

Citations 5

Authors

Tomasz Goral

Kinga Stuper-Szablewska

Maciej Busko

Maja Boczkowska

Dorota Walentyn-Goral

Halina Wisniewska

Juliusz Perkowski

Affiliations

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Abstract

Fusarium head blight is one of the most important and most common diseases of winter wheat. In order to better understanding this disease and to assess the correlations between different factors, 30 cultivars of this cereal were evaluated in a two-year period. Fusarium head blight resistance was evaluated and the concentration of trichothecene mycotoxins was analysed. Grain samples originated from plants inoculated with Fusarium culmorum and naturally infected with Fusarium species. The genetic distance between the tested cultivars was determined and data were analysed using multivariate data analysis methods. Genetic dissimilarity of wheat cultivars ranged between 0.06 and 0.78. They were grouped into three distinct groups after cluster analysis of genetic distance. Wheat cultivars differed in resistance to spike and kernel infection and in resistance to spread of Fusarium within a spike (type II). Only B trichothecenes (deoxynivalenol, 3-acetyldeoxynivalenol and nivalenol) produced by F. culmorum in grain samples from inoculated plots were present. In control samples trichothecenes of groups A (H-2 toxin, T-2 toxin, T-2 tetraol, T-2 triol, scirpentriol, diacetoxyscirpenol) and B were detected. On the basis of Fusarium head blight assessment and analysis of trichothecene concentration in the grain relationships between morphological characters, Fusarium head blight resistance and mycotoxins in grain of wheat cultivars were examined. The results were used to create of matrices of distance between cultivars - for trichothecene concentration in inoculated and naturally infected grain as well as for FHB resistance Correlations between genetic distance versus resistance/mycotoxin profiles were calculated using the Mantel test. A highly significant correlation between genetic distance and mycotoxin distance was found for the samples inoculated with Fusarium culmorum. Significant but weak relationships were found between genetic distance matrix and FHB resistance or trichothecene concentration in naturally infected grain matrices.

Citing Articles

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References

Wisniewska H, Kowalczyk K . Resistance of cultivars and breeding lines of spring wheat to Fusarium culmorum and powdery mildew. J Appl Genet. 2005; 46(1):35-40. View

Lemmens M, Scholz U, Berthiller F, DallAsta C, Koutnik A, Schuhmacher R . The ability to detoxify the mycotoxin deoxynivalenol colocalizes with a major quantitative trait locus for Fusarium head blight resistance in wheat. Mol Plant Microbe Interact. 2006; 18(12):1318-24. DOI: 10.1094/MPMI-18-1318. View

Busko M, Kulik T, Ostrowska A, Goral T, Perkowski J . Quantitative volatile compound profiles in fungal cultures of three different Fusarium graminearum chemotypes. FEMS Microbiol Lett. 2014; 359(1):85-93. DOI: 10.1111/1574-6968.12569. View

Cowger C, Patton-Ozkurt J, Brown-Guedira G, Perugini L . Post-anthesis moisture increased Fusarium head blight and deoxynivalenol levels in North Carolina winter wheat. Phytopathology. 2009; 99(4):320-7. DOI: 10.1094/PHYTO-99-4-0320. View

Berthiller F, DallAsta C, Schuhmacher R, Lemmens M, Adam G, Krska R . Masked mycotoxins: determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography-tandem mass spectrometry. J Agric Food Chem. 2005; 53(9):3421-5. DOI: 10.1021/jf047798g. View

Perkowski J, Kiecana I, Stachowiak J, Basinski T . Natural occurrence of scirpentriol in cereals infected by Fusarium species. Food Addit Contam. 2003; 20(6):572-8. DOI: 10.1080/0265203031000100773. View

Schollenberger M, Drochner W, Muller H . Fusarium toxins of the scirpentriol subgroup: a review. Mycopathologia. 2007; 164(3):101-18. DOI: 10.1007/s11046-007-9036-5. View

Miller J . Mycotoxins in small grains and maize: old problems, new challenges. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008; 25(2):219-30. DOI: 10.1080/02652030701744520. View

Stepien L, Popiel D, Koczyk G, Chelkowski J . Wheat-infecting Fusarium species in Poland--their chemotypes and frequencies revealed by PCR assay. J Appl Genet. 2008; 49(4):433-41. DOI: 10.1007/BF03195644. View

10.

Shen J, Ding X, Liu D, Ding G, He J, Li X . Intersimple sequence repeats (ISSR) molecular fingerprinting markers for authenticating populations of Dendrobium officinale Kimura et Migo. Biol Pharm Bull. 2006; 29(3):420-2. DOI: 10.1248/bpb.29.420. View

11.

Schollenberger M, Muller H, Rufle M, Suchy S, Plank S, Drochner W . Natural occurrence of 16 fusarium toxins in grains and feedstuffs of plant origin from Germany. Mycopathologia. 2006; 161(1):43-52. DOI: 10.1007/s11046-005-0199-7. View

12.

Gunnaiah R, Kushalappa A, Duggavathi R, Fox S, Somers D . Integrated metabolo-proteomic approach to decipher the mechanisms by which wheat QTL (Fhb1) contributes to resistance against Fusarium graminearum. PLoS One. 2012; 7(7):e40695. PMC: 3398977. DOI: 10.1371/journal.pone.0040695. View

13.

Carvalho A, Lima-Brito J, Macas B, Guedes-Pinto H . Genetic diversity and variation among botanical varieties of old Portuguese wheat cultivars revealed by ISSR assays. Biochem Genet. 2009; 47(3-4):276-94. DOI: 10.1007/s10528-009-9227-5. View

14.

Kokkonen M, Jestoi M, Laitila A . Mycotoxin production of Fusarium langsethiae and Fusarium sporotrichioides on cereal-based substrates. Mycotoxin Res. 2013; 28(1):25-35. DOI: 10.1007/s12550-011-0113-8. View

15.

Mitrofanova O, Strelchenko P, Konarev A, Balfourier F . [Genetic differentiation of hexaploid wheat inferred from analysis of microsatellite loci]. Genetika. 2010; 45(11):1530-9. View

16.

Edwards S, Imathiu S, Ray R, Back M, Hare M . Molecular studies to identify the Fusarium species responsible for HT-2 and T-2 mycotoxins in UK oats. Int J Food Microbiol. 2012; 156(2):168-75. DOI: 10.1016/j.ijfoodmicro.2012.03.020. View

17.

Srinivasachary , Gosman N, Steed A, Hollins T, Bayles R, Jennings P . Semi-dwarfing Rht-B1 and Rht-D1 loci of wheat differ significantly in their influence on resistance to Fusarium head blight. Theor Appl Genet. 2008; 118(4):695-702. DOI: 10.1007/s00122-008-0930-0. View

18.

Nielsen L, Jensen J, Rodriguez A, Jorgensen L, Justesen A . TRI12 based quantitative real-time PCR assays reveal the distribution of trichothecene genotypes of F. graminearum and F. culmorum isolates in Danish small grain cereals. Int J Food Microbiol. 2012; 157(3):384-92. DOI: 10.1016/j.ijfoodmicro.2012.06.010. View

19.

Wurschum T, Langer S, Longin C, Korzun V, Akhunov E, Ebmeyer E . Population structure, genetic diversity and linkage disequilibrium in elite winter wheat assessed with SNP and SSR markers. Theor Appl Genet. 2013; 126(6):1477-86. DOI: 10.1007/s00122-013-2065-1. View

20.

Kollers S, Rodemann B, Ling J, Korzun V, Ebmeyer E, Argillier O . Whole genome association mapping of Fusarium head blight resistance in European winter wheat (Triticum aestivum L.). PLoS One. 2013; 8(2):e57500. PMC: 3579808. DOI: 10.1371/journal.pone.0057500. View