Calcitriol Modulates the Effects of Bone Marrow-derived Mesenchymal Stem Cells on Macrophage Functions

Overview

Journal Iran J Basic Med Sci

Publisher Mashhad University of Medical Sciences

Specialty General Medicine

Date 2015 Sep 10

PMID 26351558

Citations 10

Authors

Bahman Mansouri Motlagh

Nahideh Afzale Ahangaran

Seyyed Meysam Abtahi Froushani

Affiliations

Soon will be listed here.

Abstract

Objectives: Some evidence showed that calcitriol has an important role in regulating growth and differentiation of mesenchymal stem cells (MSCs). However, the interaction between mesenchymal stem cells and macrophage is not clear yet. The current study was done to investigate the in vitro effects of calcitriol on the interactions between bone marrow-derived MSCs and rat macrophages.

Materials And Methods: MSCs were isolated from rat bone marrow and pulsed with different concentrations of calcitriol (50, 100 and 200 nanomolar) for 24, 48 and 72 hr. Then, mesenchymal stem cells were co-cultured with macrophages for 4 hr. Finally, macrophages were evaluated for ability to uptake neutral red, phagocytosis activity against opsonized yeast, respiratory burst and viability.

Results: Our data showed that bone marrow-derived MSCs pulsed with calcitriol may cause a significant increase in uptake of neutral red and phagocytic activity of opsonized heat killed baker's yeast. Moreover, treatment of MSCs with calcitriol enhanced macrophage viability. Nevertheless, the respiratory burst of macrophages was significantly reduced in macrophages co-cultured with calcitriol-treated MSCs compared to control group.

Conclusion: Calcitriol may accelerate and potentiate anti-inflammatory M2 macrophage polarization by MSCs.

Citing Articles

Therapeutic Effects of IL-1RA, M2 Cells, and Their Synergistic Impact on a Mouse Model of Rheumatoid Arthritis.

Hashemzadeh M, Esmaeili Gouvarchin Ghaleh H, Mohammadi M, Yousefpoor Y, Rezaei E, Alishiri G Adv Pharm Bull. 2024; 14(2):483-495.

PMID: 39206397 PMC: 11347727. DOI: 10.34172/apb.2024.037.

Ex Vivo Immune Function and Modulatory Effects of Calcitriol in Dogs with Naturally Occurring Diabetes Mellitus.

Jaffey J, Kreisler R, Graves T, Al-Nakkash L, Backus R, Allison L Vet Sci. 2024; 11(5).

PMID: 38787165 PMC: 11125998. DOI: 10.3390/vetsci11050193.

Benefits of bone marrow-derived mesenchymal stem cells primed with estradiol in alleviating collagen-induced arthritis.

Jahantigh M, Froushani S, Afzale Ahangaran N Iran J Basic Med Sci. 2023; 26(4):400-407.

PMID: 37009006 PMC: 10008397. DOI: 10.22038/IJBMS.2023.68112.14882.

An evaluation of the effects of Pistacia atlantica gum hydro-alcoholic extract on the phagocytosis ability of macrophages and atherosclerosis development in hypercholesteremic rats.

Ghalavand M, Esmaeili-Gouvarchin-Ghaleh H, Mirzaei-Nodooshan M, Vazifedost S, Mohammadi-Yeganeh S ARYA Atheroscler. 2023; 18(4):1-6.

PMID: 36817347 PMC: 9937666. DOI: 10.22122/arya.2022.11866.0.

Evaluation of serum 25-hydroxyvitamin D, C-reactive protein, and haptoglobin as biomarkers in dogs newly diagnosed with histoplasmosis.

Hernandez B, Jaffey J, Cohn L, Backus R, KuKanich K, Hanzlicek A J Vet Intern Med. 2023; 37(2):476-483.

PMID: 36748822 PMC: 10061178. DOI: 10.1111/jvim.16640.

References

Froushani S, Esmaili Gourvarchin Galeh H . New insight into the immunomodulatory mechanisms of Tretinoin in NMRI mice. Iran J Basic Med Sci. 2015; 17(9):632-7. PMC: 4322144. View

Babior B . Phagocytes and oxidative stress. Am J Med. 2000; 109(1):33-44. DOI: 10.1016/s0002-9343(00)00481-2. View

Ghannam S, Bouffi C, Djouad F, Jorgensen C, Noel D . Immunosuppression by mesenchymal stem cells: mechanisms and clinical applications. Stem Cell Res Ther. 2010; 1(1):2. PMC: 2873698. DOI: 10.1186/scrt2. View

Nabi A, Islam L, Rahman M, Biswas K . Polymorphonuclear neutrophil dysfunctions in streptozotocin-induced type 1 diabetic rats. J Biochem Mol Biol. 2005; 38(6):661-7. DOI: 10.5483/bmbrep.2005.38.6.661. View

Artaza J, Sirad F, Ferrini M, Norris K . 1,25(OH)2vitamin D3 inhibits cell proliferation by promoting cell cycle arrest without inducing apoptosis and modifies cell morphology of mesenchymal multipotent cells. J Steroid Biochem Mol Biol. 2010; 119(1-2):73-83. PMC: 2828517. DOI: 10.1016/j.jsbmb.2010.01.001. View

Newman S, Holly A . Candida albicans is phagocytosed, killed, and processed for antigen presentation by human dendritic cells. Infect Immun. 2001; 69(11):6813-22. PMC: 100059. DOI: 10.1128/IAI.69.11.6813-6822.2001. View

Wada N, Gronthos S, Bartold P . Immunomodulatory effects of stem cells. Periodontol 2000. 2013; 63(1):198-216. DOI: 10.1111/prd.12024. View

Raffaghello L, Bianchi G, Bertolotto M, Montecucco F, Busca A, Dallegri F . Human mesenchymal stem cells inhibit neutrophil apoptosis: a model for neutrophil preservation in the bone marrow niche. Stem Cells. 2007; 26(1):151-62. DOI: 10.1634/stemcells.2007-0416. View

Froushani S, Delirezh N, Hobbenaghi R, Mosayebi G . Synergistic effects of atorvastatin and all-trans retinoic acid in ameliorating animal model of multiple sclerosis. Immunol Invest. 2013; 43(1):54-68. DOI: 10.3109/08820139.2013.825269. View

10.

da Silva Meirelles L, Maria Fontes A, Covas D, Caplan A . Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine Growth Factor Rev. 2009; 20(5-6):419-27. DOI: 10.1016/j.cytogfr.2009.10.002. View

11.

Uccelli A, Moretta L, Pistoia V . Mesenchymal stem cells in health and disease. Nat Rev Immunol. 2009; 8(9):726-36. DOI: 10.1038/nri2395. View

12.

Maqbool M, Vidyadaran S, George E, Ramasamy R . Human mesenchymal stem cells protect neutrophils from serum-deprived cell death. Cell Biol Int. 2011; 35(12):1247-51. DOI: 10.1042/CBI20110070. View

13.

Kim J, Hematti P . Mesenchymal stem cell-educated macrophages: a novel type of alternatively activated macrophages. Exp Hematol. 2009; 37(12):1445-53. PMC: 2783735. DOI: 10.1016/j.exphem.2009.09.004. View

14.

Yi T, Song S . Immunomodulatory properties of mesenchymal stem cells and their therapeutic applications. Arch Pharm Res. 2012; 35(2):213-21. DOI: 10.1007/s12272-012-0202-z. View

15.

Klotz B, Mentrup B, Regensburger M, Zeck S, Schneidereit J, Schupp N . 1,25-dihydroxyvitamin D3 treatment delays cellular aging in human mesenchymal stem cells while maintaining their multipotent capacity. PLoS One. 2012; 7(1):e29959. PMC: 3252365. DOI: 10.1371/journal.pone.0029959. View

16.

Hamaliaka A, Novikova I . Nitric oxide production disorders in leukocytes of patients with recurrent furunculosis. Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2010; 154(2):163-7. DOI: 10.5507/bp.2010.025. View

17.

Greenberg S, Grinstein S . Phagocytosis and innate immunity. Curr Opin Immunol. 2002; 14(1):136-45. DOI: 10.1016/s0952-7915(01)00309-0. View

18.

Smyk D, Orfanidou T, Invernizzi P, Bogdanos D, Lenzi M . Vitamin D in autoimmune liver disease. Clin Res Hepatol Gastroenterol. 2013; 37(5):535-45. DOI: 10.1016/j.clinre.2013.05.016. View

19.

Zhang R, Liu Y, Yan K, Chen L, Chen X, Li P . Anti-inflammatory and immunomodulatory mechanisms of mesenchymal stem cell transplantation in experimental traumatic brain injury. J Neuroinflammation. 2013; 10:106. PMC: 3765323. DOI: 10.1186/1742-2094-10-106. View

20.

Bouillon R, Carmeliet G, Verlinden L, van Etten E, Verstuyf A, Luderer H . Vitamin D and human health: lessons from vitamin D receptor null mice. Endocr Rev. 2008; 29(6):726-76. PMC: 2583388. DOI: 10.1210/er.2008-0004. View