Cocaine and Metabolite Concentrations in DBS and Venous Blood After Controlled Intravenous Cocaine Administration

Overview

Journal Bioanalysis

Specialty Chemistry

Date 2015 Sep 2

PMID 26327184

Citations 9

Authors

Kayla N Ellefsen

Jose Luiz da Costa

Marta Concheiro

Sebastien Anizan

Allan J Barnes

Sandrine Pirard

David A Gorelick

Marilyn A Huestis

Affiliations

Soon will be listed here.

Abstract

Background: DBS are an increasingly common clinical matrix.

Methods & Results: Sensitive and specific methods for DBS and venous blood cocaine and metabolite detection by LC-HRMS and 2D GC-MS, respectively, were validated to examine correlation between concentrations following controlled intravenous cocaine administration. Linear ranges from 1 to 200 µg/l were achieved, with acceptable bias and imprecision. Authentic matched specimens' (392 DBS, 97 venous blood) cocaine and benzoylecgonine concentrations were qualitatively similar, but DBS had much greater variability (21.4-105.9 %CV) and were lower than in blood.

Conclusion: DBS offer advantages for monitoring cocaine intake; however, differences between capillary and venous blood and DBS concentration variability must be addressed.

Citing Articles

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