Inntags: Small Self-structured Epitopes for Innocuous Protein Tagging

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2015 Sep 1

PMID 26322837

Citations 13

Authors

Maya V Georgieva

Galal Yahya

Laia Codo

Raul Ortiz

Laura Teixido

Jose Claros

Ricardo Jara

Monica Jara

Antoni Iborra

Josep Lluis Gelpi

Carme Gallego

Modesto Orozco

Marti Aldea

Affiliations

Soon will be listed here.

Abstract

Protein tagging is widely used in approaches ranging from affinity purification to fluorescence-based detection in live cells. However, an intrinsic limitation of tagging is that the native function of the protein may be compromised or even abolished by the presence of the tag. Here we describe and characterize a set of small, innocuous protein tags (inntags) that we anticipate will find application in a variety of biological techniques.

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