In Vitro Antimicrobial Activity of Plants Used in Traditional Medicine in Gurage and Silti Zones, South Central Ethiopia

Overview

Journal BMC Complement Altern Med

Publisher Biomed Central

Specialty Rehabilitation Medicine

Date 2015 Aug 19

PMID 26283630

Citations 15

Authors

Alemtshay Teka

Johana Rondevaldova

Zemede Asfaw

Sebsebe Demissew

Patrick Van Damme

Ladislav Kokoska

Wouter Vanhove

Affiliations

Soon will be listed here.

Abstract

Background: To overcome the escalating problems associated with infectious diseases and drug resistance, discovery of new antimicrobials is crucial. The present study aimed to carry out in vitro antimicrobial analysis of 15 medicinal plant species selected according to their traditional medicinal uses in Gurage and Silti Zones, south central Ethiopia.

Methods: Ethanol extracts of various plant parts were investigated for their antimicrobial activity against 20 bacterial and one yeast strains. The minimum inhibitory concentration (MIC) was determined by broth microdilution method.

Results: Asparagus africanus, Guizotia schimperi, Lippia adoensis var. adoensis and Premna schimperi were active against Candida albicans, Enterococcus faecalis and Staphylococcus aureus at a concentration of 512 μg/ml or lower. Strong antibacterial activity (MIC≥128 μg/ml) was observed for G. schimperi extract against 17 resistant and sensitive Staphylococcus strains, at a concentration comparable to standard antibiotics. Moreover, this extract showed higher antibacterial activity for the test against S. aureus ATCC 33591, ATCC 33592, SA3 and SA5 strains (128-256 μg/ml) than oxacillin (512 μg/ml).

Conclusions: The study revealed in vitro antibacterial activity of plants used in folk medicine in south central Ethiopia. The usefulness of these plants, in particular of G. schimperi, should be confirmed through further phytochemical and toxicity analyses.

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References

Giday M, Asfaw Z, Elmqvist T, Woldu Z . An ethnobotanical study of medicinal plants used by the Zay people in Ethiopia. J Ethnopharmacol. 2003; 85(1):43-52. DOI: 10.1016/s0378-8741(02)00359-8. View

Bekalo T, Woodmatas S, Woldemariam Z . An ethnobotanical study of medicinal plants used by local people in the lowlands of Konta Special Woreda, southern nations, nationalities and peoples regional state, Ethiopia. J Ethnobiol Ethnomed. 2009; 5:26. PMC: 2764637. DOI: 10.1186/1746-4269-5-26. View

Tafesse G, Mekonnen Y, Makonnen E . Antifertility effect of aqueous and ethanol extracts of the leaves and roots of Asparagus africanus in rats. Afr Health Sci. 2006; 6(2):81-5. PMC: 2567240. DOI: 10.5555/afhs.2006.6.2.81. View

Cos P, Vlietinck A, Vanden Berghe D, Maes L . Anti-infective potential of natural products: how to develop a stronger in vitro 'proof-of-concept'. J Ethnopharmacol. 2006; 106(3):290-302. DOI: 10.1016/j.jep.2006.04.003. View

Negi J, Singh P, Joshi G, Rawat M, Bisht V . Chemical constituents of Asparagus. Pharmacogn Rev. 2012; 4(8):215-20. PMC: 3249924. DOI: 10.4103/0973-7847.70921. View

Motamedi H, Darabpour E, Gholipour M, Seyyed Nejad S . In vitro assay for the anti-Brucella activity of medicinal plants against tetracycline-resistant Brucella melitensis. J Zhejiang Univ Sci B. 2010; 11(7):506-11. PMC: 2897020. DOI: 10.1631/jzus.B0900365. View

Taye B, Giday M, Animut A, Seid J . Antibacterial activities of selected medicinal plants in traditional treatment of human wounds in Ethiopia. Asian Pac J Trop Biomed. 2013; 1(5):370-5. PMC: 3614199. DOI: 10.1016/S2221-1691(11)60082-8. View

Tadeg H, Mohammed E, Asres K, Gebre-Mariam T . Antimicrobial activities of some selected traditional Ethiopian medicinal plants used in the treatment of skin disorders. J Ethnopharmacol. 2005; 100(1-2):168-75. DOI: 10.1016/j.jep.2005.02.031. View

Cowan M . Plant products as antimicrobial agents. Clin Microbiol Rev. 1999; 12(4):564-82. PMC: 88925. DOI: 10.1128/CMR.12.4.564. View

10.

Madikizela B, Ndhlala A, Finnie J, van Staden J . In vitro antimicrobial activity of extracts from plants used traditionally in South Africa to treat tuberculosis and related symptoms. Evid Based Complement Alternat Med. 2013; 2013:840719. PMC: 3603154. DOI: 10.1155/2013/840719. View

11.

Mulu A, Moges F, Tessema B, Kassu A . Pattern and multiple drug resistance of bacterial pathogens isolated from wound infection at University of Gondar Teaching Hospital, Northwest Ethiopia. Ethiop Med J. 2007; 44(2):125-31. View

12.

Savoia D . Plant-derived antimicrobial compounds: alternatives to antibiotics. Future Microbiol. 2012; 7(8):979-90. DOI: 10.2217/fmb.12.68. View

13.

de Albuquerque U . Re-examining hypotheses concerning the use and knowledge of medicinal plants: a study in the Caatinga vegetation of NE Brazil. J Ethnobiol Ethnomed. 2006; 2:30. PMC: 1557484. DOI: 10.1186/1746-4269-2-30. View

14.

Lulekal E, Rondevaldova J, Bernaskova E, Cepkova J, Asfaw Z, Kelbessa E . Antimicrobial activity of traditional medicinal plants from Ankober District, North Shewa Zone, Amhara Region, Ethiopia. Pharm Biol. 2014; 52(5):614-20. DOI: 10.3109/13880209.2013.858362. View

15.

Ramesha B, Suma H, Senthilkumar U, Priti V, Ravikanth G, Vasudeva R . New plant sources of the anti-cancer alkaloid, camptothecine from the Icacinaceae taxa, India. Phytomedicine. 2013; 20(6):521-7. DOI: 10.1016/j.phymed.2012.12.003. View

16.

Mukazayire M, Allaeys V, Buc Calderon P, Stevigny C, Bigendako M, Duez P . Evaluation of the hepatotoxic and hepatoprotective effect of Rwandese herbal drugs on in vivo (guinea pigs barbiturate-induced sleeping time) and in vitro (rat precision-cut liver slices, PCLS) models. Exp Toxicol Pathol. 2009; 62(3):289-99. DOI: 10.1016/j.etp.2009.04.005. View

17.

Habtemariam S, Gray A, Halbert G, Waterman P . A novel antibacterial diterpene from Premna schimperi. Planta Med. 1990; 56(2):187-9. DOI: 10.1055/s-2006-960922. View

18.

Parker M, Chabot S, Ward B, Johns T . Traditional dietary additives of the Maasai are antiviral against the measles virus. J Ethnopharmacol. 2007; 114(2):146-52. DOI: 10.1016/j.jep.2007.06.011. View

19.

Alamri S, Moustafa M . Antimicrobial properties of 3 medicinal plants from Saudi Arabia against some clinical isolates of bacteria. Saudi Med J. 2012; 33(3):272-7. View

20.

Noge K, Becerra J . Germacrene D, a common sesquiterpene in the genus Bursera (Burseraceae). Molecules. 2009; 14(12):5289-97. PMC: 6255432. DOI: 10.3390/molecules14125289. View