Expression of Poly(C)-binding Protein 1 (PCBP1) in NSCLC As a Negative Regulator of EMT and Its Clinical Value

Overview

Journal Int J Clin Exp Pathol

Specialty Pathology

Date 2015 Aug 12

PMID 26261610

Citations 21

Authors

Yifei Liu

Ling Gai

Jian Liu

Yuan Cui

Yan Zhang

Jia Feng

Affiliations

Soon will be listed here.

Abstract

Poly (C)-binding Protein 1 (PCBP1) is a 35 kDa protein involved in a number of biological processes. Recently, the research found that PCBP1 might be involved in epithelial-mesenchymal transition (EMT). However, the role of PCBP1 in non-small-cell lung cancer (NSCLC) metastasis needs further elucidation. The purpose of this study was to determine whether PCBP1 could serve as a biomarker for stratification and prediction of prognosis in NSCLC as a regulator of EMT formation. In this study, PCBP1 expression was evaluated by Western blot in 8 fresh lung cancer tissues and immunohistochemistry (IHC) on 145 paraffin-embedded slices. PCBP1 was highly expressed in non-metastatic NSCLC specimens and significantly correlated with lymph node status (P < 0.001), clinical stage (P = 0.001), vimentin expression (P = 0.033) and E-cadherin expression (P = 0.042). Our study showed that the low expression of PCBP1 was correlated with decreased expression of E-cadherin and elevated expression of vimentin, which were the markers of EMT. Besides, high expression of PCBP1 was correlated with better prognosis. These findings suggested that PCBP1 might play an important role in preventing the process of EMT in NSCLC, thus be a promising therapeutic target to inhibit NSCLC metastasis.

Citing Articles

PCBP1 regulates LIFR through FAM3C to maintain breast cancer stem cell self-renewal and invasiveness.

Streitfeld W, Dalton A, Howley B, Howe P Cancer Biol Ther. 2023; 24(1):2271638.

PMID: 37927213 PMC: 10629429. DOI: 10.1080/15384047.2023.2271638.

Iron as spirit of life to share under monopoly.

Toyokuni S, Kong Y, Zheng H, Maeda Y, Motooka Y, Akatsuka S J Clin Biochem Nutr. 2022; 71(2):78-88.

PMID: 36213789 PMC: 9519419. DOI: 10.3164/jcbn.22-43.

Overexpression of splicing factor poly(rC)-binding protein 1 elicits cycle arrest, apoptosis induction, and p73 splicing in human cervical carcinoma cells.

Chen Y, Dou Z, Chen X, Zhao D, Che T, Su W J Cancer Res Clin Oncol. 2022; 148(12):3475-3484.

PMID: 35896897 DOI: 10.1007/s00432-022-04170-3.

Baicalin Inhibits EMT through PDK1/AKT Signaling in Human Nonsmall Cell Lung Cancer.

Chen J, Yuan C, Yang B, Zhou X J Oncol. 2021; 2021:4391581.

PMID: 34868313 PMC: 8639265. DOI: 10.1155/2021/4391581.

miR-135a inhibits malignant proliferation and diffusion of non-small cell lung cancer cells by down-regulating ROCK1 protein.

Zhao Y, Sun X, Zhu K, Cheng M Biosci Rep. 2020; 40(6).

PMID: 32484204 PMC: 7295638. DOI: 10.1042/BSR20201276.

References

Yang J, Weinberg R . Epithelial-mesenchymal transition: at the crossroads of development and tumor metastasis. Dev Cell. 2008; 14(6):818-29. DOI: 10.1016/j.devcel.2008.05.009. View

Meng Q, Rayala S, Gururaj A, Talukder A, OMalley B, Kumar R . Signaling-dependent and coordinated regulation of transcription, splicing, and translation resides in a single coregulator, PCBP1. Proc Natl Acad Sci U S A. 2007; 104(14):5866-71. PMC: 1851583. DOI: 10.1073/pnas.0701065104. View

Choi H, Hwang C, Song K, Law P, Wei L, Loh H . Poly(C)-binding proteins as transcriptional regulators of gene expression. Biochem Biophys Res Commun. 2009; 380(3):431-6. PMC: 2657093. DOI: 10.1016/j.bbrc.2009.01.136. View

Iwatsuki M, Mimori K, Yokobori T, Ishi H, Beppu T, Nakamori S . Epithelial-mesenchymal transition in cancer development and its clinical significance. Cancer Sci. 2009; 101(2):293-9. PMC: 11159985. DOI: 10.1111/j.1349-7006.2009.01419.x. View

Zhang T, Huang X, Dong L, Hu D, Ge C, Zhan Y . PCBP-1 regulates alternative splicing of the CD44 gene and inhibits invasion in human hepatoma cell line HepG2 cells. Mol Cancer. 2010; 9:72. PMC: 2864215. DOI: 10.1186/1476-4598-9-72. View

Wang H, Vardy L, Tan C, Loo J, Guo K, Li J . PCBP1 suppresses the translation of metastasis-associated PRL-3 phosphatase. Cancer Cell. 2010; 18(1):52-62. DOI: 10.1016/j.ccr.2010.04.028. View

Cloke B, Shah K, Kaneda H, Lavery S, Trew G, Fusi L . The poly(c)-binding protein-1 regulates expression of the androgen receptor. Endocrinology. 2010; 151(8):3954-64. DOI: 10.1210/en.2009-1264. View

Shi Z, Zhang T, Long W, Wang X, Zhang X, Ling X . Down-regulation of poly(rC)-binding protein 1 correlates with the malignant transformation of hydatidiform moles. Int J Gynecol Cancer. 2012; 22(7):1125-9. DOI: 10.1097/IGC.0b013e3182606ac3. View

Holgersson G, Bergqvist M, Nyman J, Hoye E, Helsing M, Friesland S . The impact of hyperfractionated radiotherapy regimen in patients with non-small cell lung cancer. Med Oncol. 2012; 30(1):320. DOI: 10.1007/s12032-012-0320-y. View

10.

Song Q, Sheng W, Zhang X, Jiao S, Li F . ILEI drives epithelial to mesenchymal transition and metastatic progression in the lung cancer cell line A549. Tumour Biol. 2013; 35(2):1377-82. DOI: 10.1007/s13277-013-1188-y. View

11.

Zhang H, Dou K . PCBP1 is an important mediator of TGF-β-induced epithelial to mesenchymal transition in gall bladder cancer cell line GBC-SD. Mol Biol Rep. 2014; 41(8):5519-24. DOI: 10.1007/s11033-014-3428-7. View

12.

Xue X, Wang X, Liu Y, Teng G, Wang Y, Zang X . SchA-p85-FAK complex dictates isoform-specific activation of Akt2 and subsequent PCBP1-mediated post-transcriptional regulation of TGFβ-mediated epithelial to mesenchymal transition in human lung cancer cell line A549. Tumour Biol. 2014; 35(8):7853-9. DOI: 10.1007/s13277-014-1982-1. View

13.

Pang L, Li Q, Wei C, Zou H, Li S, Cao W . TGF-β1/Smad signaling pathway regulates epithelial-to-mesenchymal transition in esophageal squamous cell carcinoma: in vitro and clinical analyses of cell lines and nomadic Kazakh patients from northwest Xinjiang, China. PLoS One. 2014; 9(12):e112300. PMC: 4251902. DOI: 10.1371/journal.pone.0112300. View

14.

Jin D, Fang Y, Li Z, Chen Z, Xiang J . Epithelial-mesenchymal transition‑associated microRNAs in colorectal cancer and drug-targeted therapies (Review). Oncol Rep. 2014; 33(2):515-25. DOI: 10.3892/or.2014.3638. View

15.

Chen Q, Cai Z, Chen Y, Gu M, Zheng D, Zhou J . Poly r(C) binding protein-1 is central to maintenance of cancer stem cells in prostate cancer cells. Cell Physiol Biochem. 2015; 35(3):1052-61. DOI: 10.1159/000373931. View

16.

Canesin G, Cuevas E, Santos V, Lopez-Menendez C, Moreno-Bueno G, Huang Y . Lysyl oxidase-like 2 (LOXL2) and E47 EMT factor: novel partners in E-cadherin repression and early metastasis colonization. Oncogene. 2014; 34(8):951-64. DOI: 10.1038/onc.2014.23. View

17.

Lewandowska M, Czubak K, Klonowska K, Jozwicki W, Kowalewski J, Kozlowski P . The use of a two-tiered testing strategy for the simultaneous detection of small EGFR mutations and EGFR amplification in lung cancer. PLoS One. 2015; 10(2):e0117983. PMC: 4342230. DOI: 10.1371/journal.pone.0117983. View

18.

Makeyev A, Liebhaber S . The poly(C)-binding proteins: a multiplicity of functions and a search for mechanisms. RNA. 2002; 8(3):265-78. PMC: 1370249. DOI: 10.1017/s1355838202024627. View

19.

Thakur S, Nakamura T, Calin G, Russo A, Tamburrino J, Shimizu M . Regulation of BRCA1 transcription by specific single-stranded DNA binding factors. Mol Cell Biol. 2003; 23(11):3774-87. PMC: 155225. DOI: 10.1128/MCB.23.11.3774-3787.2003. View

20.

Pillai M, CHACKO P, Kesari L, Jayaprakash P, Jayaram H, Antony A . Expression of folate receptors and heterogeneous nuclear ribonucleoprotein E1 in women with human papillomavirus mediated transformation of cervical tissue to cancer. J Clin Pathol. 2003; 56(8):569-74. PMC: 1770025. DOI: 10.1136/jcp.56.8.569. View