Analysis of Picosecond Laser Induced Fluorescence Phenomena in Photosynthetic Membranes Utilizing a Master Equation Approach

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1979 Mar 1

PMID 262402

Citations 23

Authors

G Paillotin

C E Swenberg

J Breton

N E Geacintov

Affiliations

Soon will be listed here.

Abstract

A Pauli master equation is formulated and solved to describe the fluorescence quantum yield, phi, and the fluorescence temporal decay curves. F(t), obtained in picosecond laser excitation experiments of photosynthetic systems. It is assumed that the lowering of phi with increasing pulse intensity is due to bimolecular singlet exciton annihilation processes which compete with the monomolecular exciton decay processes; Poisson statistics are taken into account. Calculated curves of phi as a function of the number of photon hits per domain are compared with experimental data, and it is concluded that these domains contain at least two to four connected photosynthetic units (depending on the temperature), where each photosynthetic unit is assumed to contain approximately 300 pigment molecules. It is shown that under conditions of high excitation intensities, the fluorescence decays approximately according to the (time)1/2 law.

Citing Articles

Fluorescence polarization measures energy funneling in single light-harvesting antennas--LH2 vs conjugated polymers.

Camacho R, Tubasum S, Southall J, Cogdell R, Sforazzini G, Anderson H Sci Rep. 2015; 5:15080.

PMID: 26478272 PMC: 4609963. DOI: 10.1038/srep15080.

Singlet-triplet annihilation in single LHCII complexes.

Gruber J, Chmeliov J, Kruger T, Valkunas L, van Grondelle R Phys Chem Chem Phys. 2015; 17(30):19844-53.

PMID: 26156159 PMC: 11289710. DOI: 10.1039/c5cp01806d.

A comparison between plant photosystem I and photosystem II architecture and functioning.

Caffarri S, Tibiletti T, Jennings R, Santabarbara S Curr Protein Pept Sci. 2014; 15(4):296-331.

PMID: 24678674 PMC: 4030627. DOI: 10.2174/1389203715666140327102218.

Photoelectric study on the kinetics of trapping and charge stabilization in oriented PS II membranes.

Leibl W, Breton J, Deprez J, Trissl H Photosynth Res. 2014; 22(3):257-75.

PMID: 24424815 DOI: 10.1007/BF00048304.

Evidence of excited state absorption in PS II membrane fragments.

Bittner T, Voigt J, Kehrberg G, Eckert H, Renger G Photosynth Res. 2014; 28(3):131-9.

PMID: 24414972 DOI: 10.1007/BF00054126.

References

Mauzerall D . Multiple excitations in photosynthetic systems. Biophys J. 1976; 16(1):87-91. PMC: 1334816. DOI: 10.1016/S0006-3495(76)85665-2. View

Campillo A, SHAPIRO S, Kollman V, Winn K, Hyer R . Picosecond exciton annihilation in photosynthetic systems. Biophys J. 1976; 16(1):93-7. PMC: 1334817. DOI: 10.1016/S0006-3495(76)85666-4. View

Joliot P, Bennoun P, Joliot A . New evidence supporting energy transfer between photosynthetic units. Biochim Biophys Acta. 1973; 305(2):317-28. DOI: 10.1016/0005-2728(73)90179-5. View

Paillotin G . Transport and capture of electronic excitation energy in the photosynthetic apparatus. J Theor Biol. 1972; 36(2):223-35. DOI: 10.1016/0022-5193(72)90094-x. View

Robinson G . Excitation transfer and trapping in photosynthesis. Brookhaven Symp Biol. 1966; 19:16-48. View

Barber J, Searle G, Tredwell C . Picosecond time-resolved study of MgCl2-induced chlorophyll fluorescence yield changes from chloroplasts. Biochim Biophys Acta. 1978; 501(2):174-82. DOI: 10.1016/0005-2728(78)90024-5. View

Geacintov N, Breton J . Exciton annihilation in the two photosystems in chloroplasts at 100 degrees K. Biophys J. 1977; 17(1):1-15. PMC: 1473232. DOI: 10.1016/S0006-3495(77)85623-3. View

Porter G, Synowiec J, Tredwell C . Intensity effects on the fluorescence of in vivo chlorophyll. Biochim Biophys Acta. 1977; 459(3):329-36. DOI: 10.1016/0005-2728(77)90034-2. View

Searle G, Barber J, Harris L, Porter G, Tredwell C . Picosecond laser study of fluorescence lifetimes in spinach chloroplast photosytem I and photosystem II preparations. Biochim Biophys Acta. 1977; 459(3):390-401. DOI: 10.1016/0005-2728(77)90040-8. View

10.

Geacintov N, Breton J, Swenberg C, Campillo A, Hyer R, SHAPIRO S . Picosecond and microsecond pulse laser studies of exciton quenching and exciton distribution in spinach chloroplasts at low temperatures. Biochim Biophys Acta. 1977; 461(2):306-12. DOI: 10.1016/0005-2728(77)90180-3. View

11.

Beddard G, Porter G . Excited state annihilation in the photosynthetic unit. Biochim Biophys Acta. 1977; 462(1):63-72. DOI: 10.1016/0005-2728(77)90189-x. View

12.

Paillotin G . Capture frequency of excitations and energy transfer between photosynthetic units in the photosystem II. J Theor Biol. 1976; 58(1):219-35. DOI: 10.1016/0022-5193(76)90149-1. View

13.

Swenberg C, Geacintov N, Pope M . Bimolecular quenching of excitons and fluorescence in the photosynthetic unit. Biophys J. 1976; 16(12):1447-52. PMC: 1334974. DOI: 10.1016/S0006-3495(76)85786-4. View

14.

Breton J, Geacintov N . Quenching of fluorescence of chlorophyll in vivo by long-lived excited states. FEBS Lett. 1976; 69(1):86-9. DOI: 10.1016/0014-5793(76)80659-x. View

15.

Harris L, Porter G, Synowiec J, Tredwell C, Barber J . Fluorescence lifetimes of Chlorella pyrenoidosa. Biochim Biophys Acta. 1976; 449(3):329-39. DOI: 10.1016/0005-2728(76)90145-6. View

16.

Pearlstein R . Migration and trapping of excitation quanta in photosynthetic units. Brookhaven Symp Biol. 1966; 19:8-15. View

17.

Joliot A, Joliot P . [KINETIC STUDY OF THE POTOCHEMICAL REACTION LIBERATING OXYGEN DURING PHOTOSYNTHESIS]. C R Hebd Seances Acad Sci. 1964; 258:4622-5. View

18.

Campillo A, Hyer R, Monger T, Parson W, SHAPIRO S . Light collection and harvesting processes in bacterial photosynthesis investigated on a picosecond time scale. Proc Natl Acad Sci U S A. 1977; 74(5):1997-2001. PMC: 431060. DOI: 10.1073/pnas.74.5.1997. View

19.

Campillo A, Kollman V, SHAPIRO S . Intensity Dependence of the Fluorescence Lifetime of in vivo Chlorophyll Excited by a Picosecond Light Pulse. Science. 1976; 193(4249):227-9. DOI: 10.1126/science.193.4249.227. View