MCM8-9 Complex Promotes Resection of Double-strand Break Ends by MRE11-RAD50-NBS1 Complex

Overview

Journal Nat Commun

Specialty Biology

Date 2015 Jul 29

PMID 26215093

Citations 55

Authors

Kyung Yong Lee

Jun-Sub Im

Etsuko Shibata

Jonghoon Park

Naofumi Handa

Stephen C Kowalczykowski

Anindya Dutta

Affiliations

Soon will be listed here.

Abstract

MCM8-9 complex is required for homologous recombination (HR)-mediated repair of double-strand breaks (DSBs). Here we report that MCM8-9 is required for DNA resection by MRN (MRE11-RAD50-NBS1) at DSBs to generate ssDNA. MCM8-9 interacts with MRN and is required for the nuclease activity and stable association of MRN with DSBs. The ATPase motifs of MCM8-9 are required for recruitment of MRE11 to foci of DNA damage. Homozygous deletion of the MCM9 found in various cancers sensitizes a cancer cell line to interstrand-crosslinking (ICL) agents. A cancer-derived point mutation or an SNP on MCM8 associated with premature ovarian failure (POF) diminishes the functional activity of MCM8. Therefore, the MCM8-9 complex facilitates DNA resection by the MRN complex during HR repair, genetic or epigenetic inactivation of MCM8 or MCM9 are seen in human cancers, and genetic inactivation of MCM8 may be the basis of a POF syndrome.

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