Differential Expression of MicroRNA-206 and Its Target Genes in Preeclampsia

Overview

Journal J Hypertens

Specialty Cardiology & Vascular Diseases

Date 2015 Jul 28

PMID 26213997

Citations 23

Authors

Christine Akehurst

Heather Y Small

Liliya Sharafetdinova

Rachel Forrest

Wendy Beattie

Catriona E Brown

Scott W Robinson

John D McClure

Lorraine M Work

David M Carty

Martin W McBride

Dilys J Freeman

Christian Delles

Affiliations

Soon will be listed here.

Abstract

Objectives: Preeclampsia is a multisystem disease that significantly contributes to maternal and foetal morbidity and mortality. In this study, we used a nonbiased microarray approach to identify novel circulating miRNAs in maternal plasma that may be associated with preeclampsia.

Methods: Plasma samples were obtained at 16 and 28 weeks of gestation from 18 women who later developed preeclampsia (cases) and 18 matched women with normotensive pregnancies (controls). We studied miRNA expression profiles in plasma and subsequently confirmed miRNA and target gene expression in placenta samples. Placental samples were obtained from an independent cohort of 19 women with preeclampsia matched with 19 women with normotensive pregnancies.

Results: From the microarray, we identified one miRNA that was significantly differentially expressed between cases and controls at 16 weeks of gestation and six miRNAs that were significantly differentially expressed at 28 weeks. Following qPCR validation, only one miR-206 was found to be significantly increased in 28-week samples in women who later developed preeclampsia (1.4-fold change ± 0.2). The trend for increase in miR-206 expression was mirrored within placental tissue from women with preeclampsia. In parallel, IGF-1, a target gene of miR-206, was also found to be downregulated (0.41 ± 0.04) in placental tissue from women with preeclampsia. miR-206 expression was also detectable in myometrium tissue and trophoblast cell lines.

Conclusion: Our pilot study has identified miRNA-206 as a novel factor upregulated in preeclampsia within the maternal circulation and in placental tissue.

Citing Articles

Circulating miRNAs and Preeclampsia: From Implantation to Epigenetics.

Giannubilo S, Cecati M, Marzioni D, Ciavattini A Int J Mol Sci. 2024; 25(3).

PMID: 38338700 PMC: 10855731. DOI: 10.3390/ijms25031418.

MicroRNA-206 Contributes to the Progression of Preeclampsia by Suppressing the Viability and Mobility of Trophocytes via the Inhibition of AGTR1.

Mo W, Jin J, Wang X, Luan W, Yan J, Long X Physiol Res. 2023; 72(5):597-606.

PMID: 38015759 PMC: 10751052. DOI: 10.33549/physiolres.935131.

Micro-RNAs in Human Placenta: Tiny Molecules, Immense Power.

Jin M, Xu Q, Li J, Xu S, Tang C Molecules. 2022; 27(18).

PMID: 36144676 PMC: 9501247. DOI: 10.3390/molecules27185943.

Evaluation of CD39, CD73, HIF-1α, and their related miRNAs expression in decidua of preeclampsia cases compared to healthy pregnant women.

Yousefzadeh Y, Soltani-Zangbar M, Kalafi L, Tarbiat A, Farid S, Aghebati-Maleki L Mol Biol Rep. 2022; 49(11):10183-10193.

PMID: 36048381 DOI: 10.1007/s11033-022-07887-z.

Expression Profiles and Interaction of MicroRNA and Transcripts in Response to Bovine Leukemia Virus Exposure.

Ma H, Lippolis J, Casas E Front Vet Sci. 2022; 9:887560.

PMID: 35928115 PMC: 9343836. DOI: 10.3389/fvets.2022.887560.

References

Vickers K, Palmisano B, Shoucri B, Shamburek R, Remaley A . MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins. Nat Cell Biol. 2011; 13(4):423-33. PMC: 3074610. DOI: 10.1038/ncb2210. View

Yue J, Guan J, Wang X, Zhang L, Yang Z, Ao Q . MicroRNA-206 is involved in hypoxia-induced pulmonary hypertension through targeting of the HIF-1α/Fhl-1 pathway. Lab Invest. 2013; 93(7):748-59. DOI: 10.1038/labinvest.2013.63. View

Luque A, Farwati A, Crovetto F, Crispi F, Figueras F, Gratacos E . Usefulness of circulating microRNAs for the prediction of early preeclampsia at first-trimester of pregnancy. Sci Rep. 2014; 4:4882. PMC: 4013938. DOI: 10.1038/srep04882. View

De Falco M, Cobellis L, Giraldi D, Mastrogiacomo A, Perna A, Colacurci N . Expression and distribution of notch protein members in human placenta throughout pregnancy. Placenta. 2006; 28(2-3):118-26. DOI: 10.1016/j.placenta.2006.03.010. View

Jansen F, Yang X, Hoelscher M, Cattelan A, Schmitz T, Proebsting S . Endothelial microparticle-mediated transfer of MicroRNA-126 promotes vascular endothelial cell repair via SPRED1 and is abrogated in glucose-damaged endothelial microparticles. Circulation. 2013; 128(18):2026-38. DOI: 10.1161/CIRCULATIONAHA.113.001720. View

Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A . Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002; 3(7):RESEARCH0034. PMC: 126239. DOI: 10.1186/gb-2002-3-7-research0034. View

Wu L, Zhou H, Lin H, Qi J, Zhu C, Gao Z . Circulating microRNAs are elevated in plasma from severe preeclamptic pregnancies. Reproduction. 2011; 143(3):389-97. DOI: 10.1530/REP-11-0304. View

Jalali S, Ramanathan G, Parthasarathy P, Aljubran S, Galam L, Yunus A . Mir-206 regulates pulmonary artery smooth muscle cell proliferation and differentiation. PLoS One. 2012; 7(10):e46808. PMC: 3468623. DOI: 10.1371/journal.pone.0046808. View

Jin W, Dong C . IL-17 cytokines in immunity and inflammation. Emerg Microbes Infect. 2015; 2(9):e60. PMC: 3820987. DOI: 10.1038/emi.2013.58. View

10.

Lewis B, Burge C, Bartel D . Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 2005; 120(1):15-20. DOI: 10.1016/j.cell.2004.12.035. View

11.

Yang Q, Lu J, Wang S, Li H, Ge Q, Lu Z . Application of next-generation sequencing technology to profile the circulating microRNAs in the serum of preeclampsia versus normal pregnant women. Clin Chim Acta. 2011; 412(23-24):2167-73. DOI: 10.1016/j.cca.2011.07.029. View

12.

Fowden A . The insulin-like growth factors and feto-placental growth. Placenta. 2003; 24(8-9):803-12. DOI: 10.1016/s0143-4004(03)00080-8. View

13.

Arroyo J, Chevillet J, Kroh E, Ruf I, Pritchard C, Gibson D . Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma. Proc Natl Acad Sci U S A. 2011; 108(12):5003-8. PMC: 3064324. DOI: 10.1073/pnas.1019055108. View

14.

Shan Z, Lin Q, Fu Y, Deng C, Zhou Z, Zhu J . Upregulated expression of miR-1/miR-206 in a rat model of myocardial infarction. Biochem Biophys Res Commun. 2009; 381(4):597-601. DOI: 10.1016/j.bbrc.2009.02.097. View

15.

Stahlhut C, Suarez Y, Lu J, Mishima Y, Giraldez A . miR-1 and miR-206 regulate angiogenesis by modulating VegfA expression in zebrafish. Development. 2012; 139(23):4356-64. PMC: 3509730. DOI: 10.1242/dev.083774. View

16.

Xu P, Zhao Y, Liu M, Wang Y, Wang H, Li Y . Variations of microRNAs in human placentas and plasma from preeclamptic pregnancy. Hypertension. 2014; 63(6):1276-84. DOI: 10.1161/HYPERTENSIONAHA.113.02647. View

17.

Falcone G, Perfetti A, Cardinali B, Martelli F . Noncoding RNAs: emerging players in muscular dystrophies. Biomed Res Int. 2014; 2014:503634. PMC: 3960514. DOI: 10.1155/2014/503634. View

18.

Lee S, Chu K, Jung K, Kim J, Huh J, Yoon H . miR-206 regulates brain-derived neurotrophic factor in Alzheimer disease model. Ann Neurol. 2012; 72(2):269-77. DOI: 10.1002/ana.23588. View

19.

Lanoix D, Lacasse A, St-Pierre J, Taylor S, Ethier-Chiasson M, Lafond J . Quantitative PCR pitfalls: the case of the human placenta. Mol Biotechnol. 2012; 52(3):234-43. DOI: 10.1007/s12033-012-9539-2. View

20.

Carty D, Siwy J, Brennand J, Zurbig P, Mullen W, Franke J . Urinary proteomics for prediction of preeclampsia. Hypertension. 2011; 57(3):561-9. DOI: 10.1161/HYPERTENSIONAHA.110.164285. View