Vibrational Imaging of Glucose Uptake Activity in Live Cells and Tissues by Stimulated Raman Scattering

Overview

Journal Angew Chem Int Ed Engl

Specialty Chemistry

Date 2015 Jul 25

PMID 26207979

Citations 61

Authors

Fanghao Hu

Zhixing Chen

Luyuan Zhang

Yihui Shen

Lu Wei

Wei Min

Affiliations

Soon will be listed here.

Abstract

Glucose is a ubiquitous energy source for most living organisms. Its uptake activity closely reflects cellular metabolic demand in various physiopathological conditions. Extensive efforts have been made to specifically image glucose uptake, such as with positron emission tomography, magnetic resonance imaging, and fluorescence microscopy, but all have limitations. A new platform to visualize glucose uptake activity in live cells and tissues is presented that involves performing stimulated Raman scattering on a novel glucose analogue labeled with a small alkyne moiety. Cancer cells with differing metabolic activities can be distinguished. Heterogeneous uptake patterns are observed with clear cell-cell variations in tumor xenograft tissues, neuronal culture, and mouse brain tissues. By offering the distinct advantage of optical resolution but without the undesirable influence of fluorophores, this method will facilitate the study of energy demands of living systems with subcellular resolution.

Citing Articles

An Activity-Based Sensing Approach to Multiplex Mapping of Labile Copper Pools by Stimulated Raman Scattering.

Jiang Y, El Khoury E, Pezacki A, Qian N, Oi M, Torrente L J Am Chem Soc. 2024; 146(49):33324-33337.

PMID: 39586074 PMC: 11844218. DOI: 10.1021/jacs.4c06296.

Recent Advances in Enhancement of Raman Scattering Intensity for Biological Applications.

Tian S, Zhang Z, Meng F, Wang Z, Luo L Chem Biomed Imaging. 2024; 1(7):575-589.

PMID: 39474139 PMC: 11504432. DOI: 10.1021/cbmi.3c00017.

Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level.

Chadha R, Guerrero J, Wei L, Sanchez L ACS Cent Sci. 2024; 10(4):758-774.

PMID: 38680555 PMC: 11046475. DOI: 10.1021/acscentsci.3c01438.

Post-click labeling enables highly accurate single cell analyses of glucose uptake ex vivo and in vivo.

Tsuchiya M, Tachibana N, Hamachi I Commun Biol. 2024; 7(1):459.

PMID: 38627603 PMC: 11021395. DOI: 10.1038/s42003-024-06164-y.

Biomedical applications, perspectives and tag design concepts in the cell - silent Raman window.

Vardaki M, Gregoriou V, Chochos C RSC Chem Biol. 2024; 5(4):273-292.

PMID: 38576725 PMC: 10989507. DOI: 10.1039/d3cb00217a.

References

Shafer-Peltier K, Haynes C, Glucksberg M, Van Duyne R . Toward a glucose biosensor based on surface-enhanced Raman scattering. J Am Chem Soc. 2003; 125(2):588-93. DOI: 10.1021/ja028255v. View

Wang D, Pascual J, Iserovich P, Yang H, Ma L, Kuang K . Functional studies of threonine 310 mutations in Glut1: T310I is pathogenic, causing Glut1 deficiency. J Biol Chem. 2003; 278(49):49015-21. DOI: 10.1074/jbc.M308765200. View

McNichols R, Cote G . Optical glucose sensing in biological fluids: an overview. J Biomed Opt. 2000; 5(1):5-16. DOI: 10.1117/1.429962. View

Raza S, Lang F, Aggarwal B, Fuller G, Wildrick D, Sawaya R . Necrosis and glioblastoma: a friend or a foe? A review and a hypothesis. Neurosurgery. 2002; 51(1):2-12; discussion 12-3. DOI: 10.1097/00006123-200207000-00002. View

Speizer L, Haugland R, KUTCHAI H . Asymmetric transport of a fluorescent glucose analogue by human erythrocytes. Biochim Biophys Acta. 1985; 815(1):75-84. DOI: 10.1016/0005-2736(85)90476-6. View

Vaupel P, Kallinowski F, Okunieff P . Blood flow, oxygen and nutrient supply, and metabolic microenvironment of human tumors: a review. Cancer Res. 1989; 49(23):6449-65. View

Prescher J, Bertozzi C . Chemistry in living systems. Nat Chem Biol. 2006; 1(1):13-21. DOI: 10.1038/nchembio0605-13. View

Lee H, Zhang W, Zhang D, Yang Y, Liu B, Barker E . Assessing cholesterol storage in live cells and C. elegans by stimulated Raman scattering imaging of phenyl-Diyne cholesterol. Sci Rep. 2015; 5:7930. PMC: 4302291. DOI: 10.1038/srep07930. View

Li J, Cheng J . Direct visualization of de novo lipogenesis in single living cells. Sci Rep. 2014; 4:6807. PMC: 4212242. DOI: 10.1038/srep06807. View

10.

Zhang D, Wang P, Slipchenko M, Cheng J . Fast vibrational imaging of single cells and tissues by stimulated Raman scattering microscopy. Acc Chem Res. 2014; 47(8):2282-90. PMC: 4139189. DOI: 10.1021/ar400331q. View

11.

Hong S, Chen T, Zhu Y, Li A, Huang Y, Chen X . Live-cell stimulated Raman scattering imaging of alkyne-tagged biomolecules. Angew Chem Int Ed Engl. 2014; 53(23):5827-31. DOI: 10.1002/anie.201400328. View

12.

Wei L, Hu F, Shen Y, Chen Z, Yu Y, Lin C . Live-cell imaging of alkyne-tagged small biomolecules by stimulated Raman scattering. Nat Methods. 2014; 11(4):410-2. PMC: 4040164. DOI: 10.1038/nmeth.2878. View

13.

Alfonso-Garcia A, Mittal R, Lee E, Potma E . Biological imaging with coherent Raman scattering microscopy: a tutorial. J Biomed Opt. 2014; 19(7):71407. PMC: 4019423. DOI: 10.1117/1.JBO.19.7.071407. View

14.

Hughes L, Rawle R, Boxer S . Choose your label wisely: water-soluble fluorophores often interact with lipid bilayers. PLoS One. 2014; 9(2):e87649. PMC: 3913624. DOI: 10.1371/journal.pone.0087649. View

15.

Rodrigues T, Serrao E, Kennedy B, Hu D, Kettunen M, Brindle K . Magnetic resonance imaging of tumor glycolysis using hyperpolarized 13C-labeled glucose. Nat Med. 2013; 20(1):93-7. PMC: 3886895. DOI: 10.1038/nm.3416. View

16.

Zanetti-Domingues L, Tynan C, Rolfe D, Clarke D, Martin-Fernandez M . Hydrophobic fluorescent probes introduce artifacts into single molecule tracking experiments due to non-specific binding. PLoS One. 2013; 8(9):e74200. PMC: 3774629. DOI: 10.1371/journal.pone.0074200. View

17.

Ji M, Orringer D, Freudiger C, Ramkissoon S, Liu X, Lau D . Rapid, label-free detection of brain tumors with stimulated Raman scattering microscopy. Sci Transl Med. 2013; 5(201):201ra119. PMC: 3806096. DOI: 10.1126/scitranslmed.3005954. View

18.

Walker-Samuel S, Ramasawmy R, Torrealdea F, Rega M, Rajkumar V, Johnson S . In vivo imaging of glucose uptake and metabolism in tumors. Nat Med. 2013; 19(8):1067-72. PMC: 5275770. DOI: 10.1038/nm.3252. View

19.

Grammel M, Hang H . Chemical reporters for biological discovery. Nat Chem Biol. 2013; 9(8):475-84. PMC: 3866016. DOI: 10.1038/nchembio.1296. View

20.

Rouhanifard S, Nordstrom L, Zheng T, Wu P . Chemical probing of glycans in cells and organisms. Chem Soc Rev. 2012; 42(10):4284-96. PMC: 3641795. DOI: 10.1039/c2cs35416k. View