Live-cell Imaging of Alkyne-tagged Small Biomolecules by Stimulated Raman Scattering

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2014 Mar 4

PMID 24584195

Citations 163

Authors

Lu Wei

Fanghao Hu

Yihui Shen

Zhixing Chen

Yong Yu

Chih-Chun Lin

Meng C Wang

Wei Min

Affiliations

Soon will be listed here.

Abstract

Sensitive and specific visualization of small biomolecules in living systems is highly challenging. We report stimulated Raman-scattering imaging of alkyne tags as a general strategy for studying a broad spectrum of small biomolecules in live cells and animals. We demonstrate this technique by tracking alkyne-bearing drugs in mouse tissues and visualizing de novo synthesis of DNA, RNA, proteins, phospholipids and triglycerides through metabolic incorporation of alkyne-tagged small precursors.

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