Does the Liposuction Method Influence the Phenotypic Characteristic of Human Adipose-derived Stem Cells?

Overview

Journal Biosci Rep

Specialty Cell Biology

Date 2015 Jul 17

PMID 26182374

Citations 9

Authors

Anna Bajek

Natalia Gurtowska

Lidia Gackowska

Izabela Kubiszewska

Magdalena Bodnar

Andrzej Marszalek

Rafal Januszewski

Jacek Michalkiewicz

Tomasz Drewa

Affiliations

Soon will be listed here.

Abstract

Adipose-derived stem cells (ASCs) possess a high differentiation and proliferation potential. However, the phenotypic characterization of ASCs is still difficult. Until now, there is no extensive analysis of ASCs markers depending on different liposuction methods. Therefore, the aim of the present study was to analyse 242 surface markers and determine the differences in the phenotypic pattern between ASCs obtained during mechanical and ultrasound-assisted liposuction. ASCs were isolated from healthy donors, due to mechanical and ultrasound-assisted liposuction and cultured in standard medium to the second passage. Differentiation potential and markers expression was evaluated to confirm the mesenchymal nature of cells. Then, the BD LyoplateTM Human Cell Surface Marker Screening Panel was used. Results shown that both population of ASCs are characterized by high expression of markers specific for ASCs: cluster of differentiation (CD)9, CD10, CD34, CD44, CD49d, CD54, CD55, CD59, CD71 and low expression of CD11a, CD11c and CD144. Moreover, we have noticed significant differences in antigen expression in 58 markers from the 242 studied. Presented study shows for the first time that different liposuction methods are not a significant factor which can influence the expression of human ASCs surface markers.

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