Stereological Study of the Effect of Black Olive Hydroalcoholic Extract on Osteoporosis in Vertebra and Tibia in Ovariectomized Rats

Overview

Journal Osteoporos Int

Specialties Endocrinology
Orthopedics

Date 2015 Jul 16

PMID 26174878

Citations 21

Authors

A Noorafshan

M H Dabbaghmanesh

N Tanideh

F Koohpeyma

R Rasooli

M Hajihoseini

M Bakhshayeshkaram

O K Hosseinabadi

Affiliations

Soon will be listed here.

Abstract

Unlabelled: A cocktail of many different antioxidants might be more effective than supplementation with a single molecule, and it closely resembles the natural environment in which active compounds were found. This is the first study well-grounded in stereological examination that showed that black olive extract effectively can ameliorate the quantitative changes of the bone structure and prevented bone loss in this osteoporosis animal model.

Introduction: The aim of this study was to quantitatively evaluate the effects of black olive extract consumption on treatment of ovariectomized (OVX) induced osteoporosis in rats. This is the first study well-grounded in stereological examination.

Methods: Ninety adult rats were allocated to control, sham-operated, OVX, and olive-supplemented OVX groups (received 250-, 500-, and 750-mg/kg body weight black olive hydroalcoholic extract orally) for 16 weeks. At the end of the experiment, blood samples were collected, and plasma levels of calcium, phosphorus, and alkaline phosphatase were assayed. Then, the specimens from both the tibia and fifth lumbar vertebra (L5) bones were processed, and stereological analysis was performed.

Results: Administration of extract resulted in decrease of alkaline phosphatase level during the treatment. After treatment of OVX rats with three doses of extract, the total number of the osteocytes revealed an increment in 500- and 750-mg/kg treated groups in comparison to the OVX group. This increment was significant only in L5. Compared to the OVX group, a significant increase was observed in the number of osteoblastsin L5 vertebra in three doses of extract-treated groups. However, this increment in tibia was statistically significant only in 750-mg/kg black olive hydroalcoholic extract-treated group. Moreover, the number of osteoclast cells were significantly decreased in vertebra and tibia in the treated groups compared to the OVX group (P < 0.05).

Conclusion: Black olive hydroalcoholic extract effectively can ameliorate the quantitative changes of the bone structure and prevented bone loss in this osteoporosis animal model. Thus, it can be a promising candidate for treatment of accelerated bone loss especially in postmenopausal osteoporosis.

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References

Santiago-Mora R, Casado-Diaz A, DE CASTRO M, Quesada-Gomez J . Oleuropein enhances osteoblastogenesis and inhibits adipogenesis: the effect on differentiation in stem cells derived from bone marrow. Osteoporos Int. 2010; 22(2):675-84. DOI: 10.1007/s00198-010-1270-x. View

Jeddi M, Roosta M, Dabbaghmanesh M, Ranjbar Omrani G, Ayatollahi S, Bagheri Z . Normative data and percentile curves of bone mineral density in healthy Iranian children aged 9-18 years. Arch Osteoporos. 2013; 8:114. DOI: 10.1007/s11657-012-0114-z. View

Fernandez-Real J, Bullo M, Moreno-Navarrete J, Ricart W, Ros E, Estruch R . A Mediterranean diet enriched with olive oil is associated with higher serum total osteocalcin levels in elderly men at high cardiovascular risk. J Clin Endocrinol Metab. 2012; 97(10):3792-8. PMC: 3462931. DOI: 10.1210/jc.2012-2221. View

Taxvig C, Specht I, Boberg J, Vinggaard A, Nellemann C . Dietary relevant mixtures of phytoestrogens inhibit adipocyte differentiation in vitro. Food Chem Toxicol. 2013; 55:265-71. DOI: 10.1016/j.fct.2012.12.060. View

Noorafshan A, Hoseini L, Karbalay-Doust S, Nadimi E . A simple stereological method for estimating the number and the volume of the pancreatic beta cells. JOP. 2012; 13(4):427-32. DOI: 10.6092/1590-8577/802. View

Zhang R, Liu Z, Li C, Hu S, Liu L, Wang J . Du-Zhong (Eucommia ulmoides Oliv.) cortex extract prevent OVX-induced osteoporosis in rats. Bone. 2008; 45(3):553-9. DOI: 10.1016/j.bone.2008.08.127. View

Muthusami S, Ramachandran I, Muthusamy B, Vasudevan G, Prabhu V, Subramaniam V . Ovariectomy induces oxidative stress and impairs bone antioxidant system in adult rats. Clin Chim Acta. 2005; 360(1-2):81-6. DOI: 10.1016/j.cccn.2005.04.014. View

Sheweita S, Khoshhal K . Calcium metabolism and oxidative stress in bone fractures: role of antioxidants. Curr Drug Metab. 2007; 8(5):519-25. DOI: 10.2174/138920007780866852. View

Lopez-Grueso R, Gambini J, Abdelaziz K, Monleon D, Diaz A, El Alami M . Early, but not late onset estrogen replacement therapy prevents oxidative stress and metabolic alterations caused by ovariectomy. Antioxid Redox Signal. 2013; 20(2):236-46. PMC: 3887441. DOI: 10.1089/ars.2012.5112. View

10.

Pines A . Guidelines and recommendations on hormone therapy in the menopause. J Midlife Health. 2011; 1(1):41-2. PMC: 3139264. DOI: 10.4103/0976-7800.66990. View

11.

Greenwald P, Anderson D, Nelson S, Taylor P . Clinical trials of vitamin and mineral supplements for cancer prevention. Am J Clin Nutr. 2007; 85(1):314S-317S. DOI: 10.1093/ajcn/85.1.314S. View

12.

Garcia-Villalba R, Larrosa M, Possemiers S, Tomas-Barberan F, Espin J . Bioavailability of phenolics from an oleuropein-rich olive (Olea europaea) leaf extract and its acute effect on plasma antioxidant status: comparison between pre- and postmenopausal women. Eur J Nutr. 2013; 53(4):1015-27. DOI: 10.1007/s00394-013-0604-9. View

13.

Karbalay-Doust S, Noorafshan A, Pourshahid S . Taxol and taurine protect the renal tissue of rats after unilateral ureteral obstruction: a stereological survey. Korean J Urol. 2012; 53(5):360-7. PMC: 3364477. DOI: 10.4111/kju.2012.53.5.360. View

14.

Nishide Y, Tadaishi M, Kobori M, Tousen Y, Kato M, Inada M . Possible role of S-equol on bone loss via amelioration of inflammatory indices in ovariectomized mice. J Clin Biochem Nutr. 2013; 53(1):41-8. PMC: 3705151. DOI: 10.3164/jcbn.12-123. View

15.

Mattfeldt T, Mall G, Gharehbaghi H, Moller P . Estimation of surface area and length with the orientator. J Microsc. 1990; 159(Pt 3):301-17. DOI: 10.1111/j.1365-2818.1990.tb03036.x. View

16.

Chen J, Hui E, Ip T, Thompson L . Dietary flaxseed enhances the inhibitory effect of tamoxifen on the growth of estrogen-dependent human breast cancer (mcf-7) in nude mice. Clin Cancer Res. 2004; 10(22):7703-11. DOI: 10.1158/1078-0432.CCR-04-1130. View

17.

Redlich K, Smolen J . Inflammatory bone loss: pathogenesis and therapeutic intervention. Nat Rev Drug Discov. 2012; 11(3):234-50. DOI: 10.1038/nrd3669. View

18.

Azizi Z, Zamani A, Omrani L, Omrani L, Dabaghmanesh M, Mohammadi A . Effects of hyperhomocysteinemia during the gestational period on ossification in rat embryo. Bone. 2009; 46(5):1344-8. DOI: 10.1016/j.bone.2009.11.027. View

19.

Claassen N, Potgieter H, Seppa M, Vermaak W, Coetzer H, van Papendorp D . Supplemented gamma-linolenic acid and eicosapentaenoic acid influence bone status in young male rats: effects on free urinary collagen crosslinks, total urinary hydroxyproline, and bone calcium content. Bone. 1995; 16(4 Suppl):385S-392S. DOI: 10.1016/s8756-3282(95)80481-1. View

20.

Campos M, Lopez-Aliaga I, Barrionuevo M, Lisbona F, Coves F . Nutritive utilization of calcium in rats: effects of dietary fat components and vitamin D3 on intestinal resected rats. J Nutr Sci Vitaminol (Tokyo). 1989; 35(5):511-21. DOI: 10.3177/jnsv.35.511. View