Effects of Selenium on Morphological Changes in Candida Utilis ATCC 9950 Yeast Cells

Overview

Journal Biol Trace Elem Res

Date 2015 Jul 14

PMID 26166197

Citations 21

Authors

Marek Kieliszek

Stanislaw Blazejak

Anna Bzducha-Wrobel

Agnieszka Kurcz

Affiliations

Soon will be listed here.

Abstract

This paper presents the results of microscopic examinations of the yeast cells cultured in yeast extract-peptone-dextrose (YPD) media supplemented with sodium selenite(IV). The analysis of the morphological changes in yeast cells aimed to determine whether the selected selenium doses and culturing time may affect this element accumulation in yeast cell structures in a form of inorganic or organic compounds, as a result of detoxification processes. The range of characteristic morphological changes in yeasts cultivated in experimental media with sodium selenite(IV) was observed, including cell shrinkage and cytoplasm thickening of the changes within vacuole structure. The processes of vacuole disintegration were observed in aging yeast cells in culturing medium, which may indicate the presence of so-called ghost cells lacking intracellular organelles The changes occurring in the morphology of yeasts cultured in media supplemented with sodium selenite were typical for stationary phase of yeast growth. From detailed microscopic observations, larger surface area of the cell (6.03 μm(2)) and yeast vacuole (2.17 μm(2)) were noticed after 24-h culturing in the medium with selenium of 20 mg Se(4+)/L. The coefficient of shape of the yeast cells cultured in media enriched with sodium selenite as well as in the control YPD medium ranged from 1.02 to 1.22. Elongation of cultivation time (up to 48 and 72 h) in the media supplemented with sodium selenite caused a reduction in the surface area of the yeast cell and vacuole due to detoxification processes.

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References

Kieliszek M, Blazejak S, Gientka I, Bzducha-Wrobel A . Accumulation and metabolism of selenium by yeast cells. Appl Microbiol Biotechnol. 2015; 99(13):5373-82. PMC: 4464373. DOI: 10.1007/s00253-015-6650-x. View

Forceville X, Touati S, Le Toumelin P, Ducros V, Laporte F, Chancerelle Y . Elements of margin of safety, toxicity and action of sodium selenite in a lipopolysaccharide rat model. J Trace Elem Med Biol. 2014; 28(3):303-10. DOI: 10.1016/j.jtemb.2014.03.010. View

Porat A, Sagiv Y, Elazar Z . A 56-kDa selenium-binding protein participates in intra-Golgi protein transport. J Biol Chem. 2000; 275(19):14457-65. DOI: 10.1074/jbc.275.19.14457. View

Martinez-Rodriguez A, Polo M, Carrascosa A . Structural and ultrastructural changes in yeast cells during autolysis in a model wine system and in sparkling wines. Int J Food Microbiol. 2002; 71(1):45-51. DOI: 10.1016/s0168-1605(01)00554-2. View

Gharieb M, Gadd G . Role of glutathione in detoxification of metal(loid)s by Saccharomyces cerevisiae. Biometals. 2004; 17(2):183-8. DOI: 10.1023/b:biom.0000018402.22057.62. View

Uden P, Boakye H, Kahakachchi C, Tyson J . Selective detection and identification of Se containing compounds--review and recent developments. J Chromatogr A. 2004; 1050(1):85-93. DOI: 10.1016/j.chroma.2004.05.077. View

Gerrard T, Telford J, Williams H . Detection of selenium deposits in Escherichia coli by electron microscopy. J Bacteriol. 1974; 119(3):1057-60. PMC: 245712. DOI: 10.1128/jb.119.3.1057-1060.1974. View

Volesky B . Biosorption of heavy metals by Saccharomyces cerevisiae. Appl Microbiol Biotechnol. 1995; 42(5):797-806. DOI: 10.1007/BF00171964. View

Dilsiz N, Celik S, Yilmaz O, Digrak M . The effects of selenium, vitamin E and their combination on the composition of fatty acids and proteins in Saccharomyces cerevisiae. Cell Biochem Funct. 1998; 15(4):265-9. DOI: 10.1002/(SICI)1099-0844(199712)15:4<265::AID-CBF750>3.0.CO;2-D. View

10.

Gharieb M, Gadd G . Evidence for the involvement of vacuolar activity in metal(loid) tolerance: vacuolar-lacking and -defective mutants of Saccharomyces cerevisiae display higher sensitivity to chromate, tellurite and selenite. Biometals. 1998; 11(2):101-6. DOI: 10.1023/a:1009221810760. View

11.

Eide D, Clark S, Nair T, Gehl M, Gribskov M, Guerinot M . Characterization of the yeast ionome: a genome-wide analysis of nutrient mineral and trace element homeostasis in Saccharomyces cerevisiae. Genome Biol. 2005; 6(9):R77. PMC: 1242212. DOI: 10.1186/gb-2005-6-9-r77. View

12.

Letavayova L, Vlckova V, Brozmanova J . Selenium: from cancer prevention to DNA damage. Toxicology. 2006; 227(1-2):1-14. DOI: 10.1016/j.tox.2006.07.017. View

13.

Letavayova L, Vlasakova D, Spallholz J, Brozmanova J, Chovanec M . Toxicity and mutagenicity of selenium compounds in Saccharomyces cerevisiae. Mutat Res. 2007; 638(1-2):1-10. DOI: 10.1016/j.mrfmmm.2007.08.009. View

14.

Mazzoni C, Falcone C . Caspase-dependent apoptosis in yeast. Biochim Biophys Acta. 2008; 1783(7):1320-7. DOI: 10.1016/j.bbamcr.2008.02.015. View

15.

Seitomer E, Balar B, He D, Copeland P, Kinzy T . Analysis of Saccharomyces cerevisiae null allele strains identifies a larger role for DNA damage versus oxidative stress pathways in growth inhibition by selenium. Mol Nutr Food Res. 2008; 52(11):1305-15. PMC: 2650619. DOI: 10.1002/mnfr.200700347. View

16.

Rosen B, Liu Z . Transport pathways for arsenic and selenium: a minireview. Environ Int. 2008; 35(3):512-5. PMC: 2719050. DOI: 10.1016/j.envint.2008.07.023. View

17.

Schmidt K, Wolfe D, Stiller B, Pearce D . Cd2+, Mn2+, Ni2+ and Se2+ toxicity to Saccharomyces cerevisiae lacking YPK9p the orthologue of human ATP13A2. Biochem Biophys Res Commun. 2009; 383(2):198-202. PMC: 3906623. DOI: 10.1016/j.bbrc.2009.03.151. View

18.

Manikova D, Vlasakova D, Loduhova J, Letavayova L, Vigasova D, Krascsenitsova E . Investigations on the role of base excision repair and non-homologous end-joining pathways in sodium selenite-induced toxicity and mutagenicity in Saccharomyces cerevisiae. Mutagenesis. 2009; 25(2):155-62. DOI: 10.1093/mutage/gep056. View

19.

McDermott J, Rosen B, Liu Z . Jen1p: a high affinity selenite transporter in yeast. Mol Biol Cell. 2010; 21(22):3934-41. PMC: 2982120. DOI: 10.1091/mbc.E10-06-0513. View

20.

Brozmanova J, Manikova D, Vlckova V, Chovanec M . Selenium: a double-edged sword for defense and offence in cancer. Arch Toxicol. 2010; 84(12):919-38. DOI: 10.1007/s00204-010-0595-8. View