Rapid Intraoperative Visualization of Breast Lesions with γ-glutamyl Hydroxymethyl Rhodamine Green

Overview

Journal Sci Rep

Specialty Science

Date 2015 Jul 14

PMID 26165706

Citations 42

Authors

Hiroki Ueo

Yoshiaki Shinden

Taro Tobo

Ayako Gamachi

Mitsuaki Udo

Hisateru Komatsu

Sho Nambara

Tomoko Saito

Masami Ueda

Hidenari Hirata

Shotaro Sakimura

Yuki Takano

Ryutaro Uchi

Junji Kurashige

Sayuri Akiyoshi

Tomohiro Iguchi

Hidetoshi Eguchi

Keishi Sugimachi

Yoko Kubota

Yuichiro Kai

Kenji Shibuta

Yuko Kijima

Heiji Yoshinaka

Shoji Natsugoe

Masaki Mori

Yoshihiko Maehara

Masayo Sakabe

Mako Kamiya

John W Kakareka

Thomas J Pohida

Peter L Choyke

Hisataka Kobayashi

Hiroaki Ueo

Yasuteru Urano

Koshi Mimori

Affiliations

Soon will be listed here.

Abstract

We previously developed γ-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) as a tool to detect viable cancer cells, based on the fact that the enzyme γ-glutamyltranspeptidase (GGT) is overexpressed on membranes of various cancer cells, but is not expressed in normal tissue. Cleavage of the probe by GGT generates green fluorescence. Here, we examined the feasibility of clinical application of gGlu-HMRG during breast-conserving surgery. We found that fluorescence derived from cleavage of gGlu-HMRG allowed easy discrimination of breast tumors, even those smaller than 1 mm in size, from normal mammary gland tissues, with 92% sensitivity and 94% specificity, within only 5 min after application. We believe this rapid, low-cost method represents a breakthrough in intraoperative margin assessment during breast-conserving surgery.

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