The Functional Role of PMP22 Gene in the Proliferation and Invasion of Osteosarcoma

Overview

Journal Med Sci Monit

Specialties General Medicine
Pathology

Date 2015 Jul 9

PMID 26154129

Citations 11

Authors

Shuyong Liu

Zhiping Chen

Affiliations

Soon will be listed here.

Abstract

Background: As the most common primary bone tumor, osteosarcoma has an improved survival rates with advancement of treatment methods. A higher rate of metastasis, however, leads to the aggravation of the disease. Studies have shown that some genes, namely osteosarcoma metastasis-related genes, participate in the process of tumor metastasis. The peripheral myelin protein 22 (PMP22) gene has recently been found to be abundantly expressed in the oncogenesis of osteosarcoma. Its detailed role and function in the tumor metastasis, however, remains unknown.

Material And Methods: The recombinant retroviral plasmid pcDNA3.1-PMP22 was constructed and used to transfect osteosarcoma cells SOSP-M, whose cell proliferation was measured by MTT method. The formation of tumor cell colony, the cell migration and invasion were also measured. The signal transduction pathway MAPK was further analyzed by Western blotting.

Results: The pcDNA3.1-PMP22 plasmid was confirmed to have a 305bp PMP22 fragment by EcoRI-XhoI dual digestion. Compared to the control group, osteosarcoma cell invasion was significantly facilitated by the transfection of pcDNA3.1-PMP22 plasmid (p<0.05). The recombinant plasmid also significantly potentiated the formation of tumor cell colony and increased the migration and invasion ability of tumor cells (p<0.05 in all cases). Phosphorylated p-ERK and p-P38 were also up-regulated by vector transfection (p<0.05).

Conclusions: Osteosarcoma metastasis-related gene PMP22 participates in the proliferation, invasion, migration and colony formation of osteosarcoma cells possibly via the MAPK signal transduction pathway, providing evidences for further investigation of metastatic mechanism of osteosarcoma.

Citing Articles

Origin and Therapies of Osteosarcoma.

Moukengue B, Lallier M, Marchandet L, Baudhuin M, Verrecchia F, Ory B Cancers (Basel). 2022; 14(14).

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Dysregulated miR-29a-3p/PMP22 Modulates Schwann Cell Proliferation and Migration During Peripheral Nerve Regeneration.

Shen Y, Cheng Z, Chen S, Zhang Y, Chen Q, Yi S Mol Neurobiol. 2021; 59(2):1058-1072.

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Inhibition of protein PMP22 enhances etoposide-induced cell apoptosis by p53 signaling pathway in Gastric Cancer.

Hou J, Wang L, Zhao J, Zhuo H, Cheng J, Chen X Int J Biol Sci. 2021; 17(12):3145-3157.

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MiR-139-5p negatively regulates PMP22 to repress cell proliferation by targeting the NF-κB signaling pathway in gastric cancer.

Hou J, Zhuo H, Chen X, Cheng J, Zheng W, Zhong M Int J Biol Sci. 2020; 16(7):1218-1229.

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Ahmat Amin M, Shimizu A, Ogita H Cancers (Basel). 2019; 11(11).

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