Salt-induced Transcription Factor MYB74 is Regulated by the RNA-directed DNA Methylation Pathway in Arabidopsis

Overview

Journal J Exp Bot

Publisher Oxford University Press

Specialty Biology

Date 2015 Jul 4

PMID 26139822

Citations 94

Authors

Rui Xu

Yuhan Wang

Hao Zheng

Wei Lu

Changai Wu

Jinguang Huang

Kang Yan

Guodong Yang

Chengchao Zheng

Affiliations

Soon will be listed here.

Abstract

Salt stress is one of the major abiotic stresses in agriculture worldwide that causes crop failure by interfering with the profile of gene expression and cell metabolism. Transcription factors and RNA-directed DNA methylation (RdDM) play an important role in the regulation of gene activation under abiotic stress in plants. This work characterized AtMYB74, a member of the R2R3-MYB gene family, which is transcriptionally regulated mainly by RdDM as a response in salt stress in Arabidopsis. Bisulphite sequencing indicated that 24-nt siRNAs target a region approximately 500bp upstream of the transcription initiation site of AtMYB74, which is heavily methylated. Levels of DNA methylation in this region were significantly reduced in wild type plants under salt stress, whereas no changes were found in RdDM mutants. Northern blot and quantitative real-time reverse transcription PCR analysis showed that the accumulation of 24-nt siRNAs was decreased in WT plants under salt stress. Further promoter deletion analysis revealed that the siRNA target region is essential for maintaining AtMYB74 expression patterns. In addition, transgenic plants overexpressing AtMYB74 displayed hypersensitivity to NaCl during seed germination. These results suggest that changes in the levels of the five 24-nt siRNAs regulate the AtMYB74 transcription factor via RdDM in response to salt stress.

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References

Furini A, Koncz C, Salamini F, Bartels D . High level transcription of a member of a repeated gene family confers dehydration tolerance to callus tissue of Craterostigma plantagineum. EMBO J. 1997; 16(12):3599-608. PMC: 1169984. DOI: 10.1093/emboj/16.12.3599. View

Cao X, Jacobsen S . Locus-specific control of asymmetric and CpNpG methylation by the DRM and CMT3 methyltransferase genes. Proc Natl Acad Sci U S A. 2002; 99 Suppl 4:16491-8. PMC: 139913. DOI: 10.1073/pnas.162371599. View

Wang M, Qin L, Xie C, Li W, Yuan J, Kong L . Induced and constitutive DNA methylation in a salinity-tolerant wheat introgression line. Plant Cell Physiol. 2014; 55(7):1354-65. DOI: 10.1093/pcp/pcu059. View

Ferreira L, Azevedo V, Maroco J, Margarida Oliveira M, Santos A . Salt Tolerant and Sensitive Rice Varieties Display Differential Methylome Flexibility under Salt Stress. PLoS One. 2015; 10(5):e0124060. PMC: 4416925. DOI: 10.1371/journal.pone.0124060. View

Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K . Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell. 2003; 15(1):63-78. PMC: 143451. DOI: 10.1105/tpc.006130. View

Gong Z, Morales-Ruiz T, Ariza R, Roldan-Arjona T, David L, Zhu J . ROS1, a repressor of transcriptional gene silencing in Arabidopsis, encodes a DNA glycosylase/lyase. Cell. 2003; 111(6):803-14. DOI: 10.1016/s0092-8674(02)01133-9. View

Denekamp M, Smeekens S . Integration of wounding and osmotic stress signals determines the expression of the AtMYB102 transcription factor gene. Plant Physiol. 2003; 132(3):1415-23. PMC: 167081. DOI: 10.1104/pp.102.019273. View

Mengiste T, Chen X, Salmeron J, Dietrich R . The BOTRYTIS SUSCEPTIBLE1 gene encodes an R2R3MYB transcription factor protein that is required for biotic and abiotic stress responses in Arabidopsis. Plant Cell. 2003; 15(11):2551-65. PMC: 280560. DOI: 10.1105/tpc.014167. View

Cao X, Aufsatz W, Zilberman D, Mette M, Huang M, Matzke M . Role of the DRM and CMT3 methyltransferases in RNA-directed DNA methylation. Curr Biol. 2003; 13(24):2212-7. DOI: 10.1016/j.cub.2003.11.052. View

10.

Schmittgen T, Jiang J, Liu Q, Yang L . A high-throughput method to monitor the expression of microRNA precursors. Nucleic Acids Res. 2004; 32(4):e43. PMC: 390315. DOI: 10.1093/nar/gnh040. View

11.

Czechowski T, Bari R, Stitt M, Scheible W, Udvardi M . Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes. Plant J. 2004; 38(2):366-79. DOI: 10.1111/j.1365-313X.2004.02051.x. View

12.

Xie Z, Johansen L, Gustafson A, Kasschau K, Lellis A, Zilberman D . Genetic and functional diversification of small RNA pathways in plants. PLoS Biol. 2004; 2(5):E104. PMC: 350667. DOI: 10.1371/journal.pbio.0020104. View

13.

Jefferson R, Kavanagh T, Bevan M . GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 1987; 6(13):3901-7. PMC: 553867. DOI: 10.1002/j.1460-2075.1987.tb02730.x. View

14.

Wassenegger M, Heimes S, Riedel L, Sanger H . RNA-directed de novo methylation of genomic sequences in plants. Cell. 1994; 76(3):567-76. DOI: 10.1016/0092-8674(94)90119-8. View

15.

Ronemus M, Galbiati M, Ticknor C, Chen J, Dellaporta S . Demethylation-induced developmental pleiotropy in Arabidopsis. Science. 1996; 273(5275):654-7. DOI: 10.1126/science.273.5275.654. View

16.

Jin H, Martin C . Multifunctionality and diversity within the plant MYB-gene family. Plant Mol Biol. 2000; 41(5):577-85. DOI: 10.1023/a:1006319732410. View

17.

Lindroth A, Cao X, Jackson J, Zilberman D, McCALLUM C, Henikoff S . Requirement of CHROMOMETHYLASE3 for maintenance of CpXpG methylation. Science. 2001; 292(5524):2077-80. DOI: 10.1126/science.1059745. View

18.

Stracke R, WERBER M, Weisshaar B . The R2R3-MYB gene family in Arabidopsis thaliana. Curr Opin Plant Biol. 2001; 4(5):447-56. DOI: 10.1016/s1369-5266(00)00199-0. View

19.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

20.

Choi Y, Gehring M, Johnson L, Hannon M, Harada J, Goldberg R . DEMETER, a DNA glycosylase domain protein, is required for endosperm gene imprinting and seed viability in arabidopsis. Cell. 2002; 110(1):33-42. DOI: 10.1016/s0092-8674(02)00807-3. View