Genome-wide Identification of R2R3-MYB Transcription Factor Subfamily Genes Involved in Salt Stress in Rice (Oryza Sativa L.)

Overview

Journal BMC Genomics

Publisher Biomed Central

Specialty Genetics

Date 2024 Aug 23

PMID 39179980

Authors

Hao-Cheng Zhang

Yuan-Hang Gong

Tao Tao

Shuai Lu

Wen-Yu Zhou

Han Xia

Xin-Yi Zhang

Qing-Qing Yang

Ming-Qiu Zhang

Lian-Min Hong

Qian-Qian Guo

Xin-Zhe Ren

Zhi-Di Yang

Xiu-Ling Cai

De-Yong Ren

Ji-Ping Gao

Su-Kui Jin

Yu-Jia Leng

Affiliations

Soon will be listed here.

Abstract

Background: R2R3-MYB transcription factors belong to one of the largest gene subfamilies in plants, and they are involved in diverse biological processes. However, the role of R2R3-MYB transcription factor subfamily genes in the response of rice (Oryza sativa L.) to salt stress has been rarely reported.

Results: In this study, we performed a genome-wide characterization and expression identification of rice R2R3-MYB transcription factor subfamily genes. We identified a total of 117 R2R3-MYB genes in rice and characterized their gene structure, chromosomal location, and cis-regulatory elements. According to the phylogenetic relationships and amino acid sequence homologies, the R2R3-MYB genes were divided into four groups. qRT-PCR of the R2R3-MYB genes showed that the expression levels of 10 genes significantly increased after 3 days of 0.8% NaCl treatment. We selected a high expression gene OsMYB2-115 for further analysis. OsMYB2-115 was highly expressed in the roots, stem, leaf, and leaf sheath. OsMYB2-115 was found to be localized in the nucleus, and the yeast hybrid assay showed that OsMYB2-115 has transcriptional activation activity.

Conclusion: This result provides important information for the functional analyses of rice R2R3-MYB transcription factor subfamily genes related to the salt stress response and reveals that OsMYB2-115 may be an important gene associated with salt tolerance in rice.

Citing Articles

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