SELENOPROTEINS. CRL2 Aids Elimination of Truncated Selenoproteins Produced by Failed UGA/Sec Decoding

Overview

Journal Science

Specialty Science

Date 2015 Jul 4

PMID 26138980

Citations 36

Authors

Hsiu-Chuan Lin

Szu-Chi Ho

Yi-Yun Chen

Kay-Hooi Khoo

Pang-Hung Hsu

Hsueh-Chi S Yen

Affiliations

Soon will be listed here.

Abstract

Selenocysteine (Sec) is translated from the codon UGA, typically a termination signal. Codon duality extends the genetic code; however, the coexistence of two competing UGA-decoding mechanisms immediately compromises proteome fidelity. Selenium availability tunes the reassignment of UGA to Sec. We report a CRL2 ubiquitin ligase-mediated protein quality-control system that specifically eliminates truncated proteins that result from reassignment failures. Exposing the peptide immediately N-terminal to Sec, a CRL2 recognition degron, promotes protein degradation. Sec incorporation destroys the degron, protecting read-through proteins from detection by CRL2. Our findings reveal a coupling between directed translation termination and proteolysis-assisted protein quality control, as well as a cellular strategy to cope with fluctuations in organismal selenium intake.

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