Automation, Consolidation, and Integration in Autoimmune Diagnostics

Overview

Journal Auto Immun Highlights

Publisher Biomed Central

Date 2015 Jul 4

PMID 26138781

Citations 10

Authors

Renato Tozzoli

Federica DAurizio

Danilo Villalta

Nicola Bizzaro

Affiliations

Soon will be listed here.

Abstract

Over the past two decades, we have witnessed an extraordinary change in autoimmune diagnostics, characterized by the progressive evolution of analytical technologies, the availability of new tests, and the explosive growth of molecular biology and proteomics. Aside from these huge improvements, organizational changes have also occurred which brought about a more modern vision of the autoimmune laboratory. The introduction of automation (for harmonization of testing, reduction of human error, reduction of handling steps, increase of productivity, decrease of turnaround time, improvement of safety), consolidation (combining different analytical technologies or strategies on one instrument or on one group of connected instruments) and integration (linking analytical instruments or group of instruments with pre- and post-analytical devices) opened a new era in immunodiagnostics. In this article, we review the most important changes that have occurred in autoimmune diagnostics and present some models related to the introduction of automation in the autoimmunology laboratory, such as automated indirect immunofluorescence and changes in the two-step strategy for detection of autoantibodies; automated monoplex immunoassays and reduction of turnaround time; and automated multiplex immunoassays for autoantibody profiling.

Citing Articles

Evaluation of a New Multiparametric Microdot Array-Based Immunoassay Panel for Systemic Autoimmune Disease Diagnosis.

Infantino M, Pavia F, Grossi V, Lari B, Benucci M, Li Gobbi F J Pers Med. 2024; 14(6).

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ANA-specific antibodies, ANA patterns, anti-ds-DNA results, and clinical diagnosis: a laboratory and clinical audit.

Anis S, Fatima A, Abdul Jabbar S, Arain T Immunol Res. 2022; 71(2):267-275.

PMID: 36456720 DOI: 10.1007/s12026-022-09347-z.

MicroRNAs (miRNAs) in Cardiovascular Complications of Rheumatoid Arthritis (RA): What Is New?.

Tanase D, Gosav E, Petrov D, Teodorescu D, Buliga-Finis O, Ouatu A Int J Mol Sci. 2022; 23(9).

PMID: 35563643 PMC: 9101033. DOI: 10.3390/ijms23095254.

Monoplex and multiplex immunoassays: approval, advancements, and alternatives.

Ahsan H Comp Clin Path. 2021; 31(2):333-345.

PMID: 34840549 PMC: 8605475. DOI: 10.1007/s00580-021-03302-4.

The clinical and the laboratory autoimmunologist: Where do we stand?.

Tozzoli R, Bizzaro N Auto Immun Highlights. 2020; 11(1):10.

PMID: 32670534 PMC: 7341566. DOI: 10.1186/s13317-020-00133-1.

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