Assaying Cell Cycle Status Using Flow Cytometry

Overview

Journal Curr Protoc Mol Biol

Specialty Molecular Biology

Date 2015 Jul 2

PMID 26131851

Citations 100

Authors

Kang Ho Kim

Joel M Sederstrom

Affiliations

Soon will be listed here.

Abstract

In this unit, two protocols are described for analyzing cell cycle status using flow cytometry. The first is based on the simultaneous analysis of proliferation-specific marker (Ki-67) and cellular DNA content, which discriminate resting/quiescent cell populations (G0 cell) and quantify cell cycle distribution (G1, S, or G2/M), respectively. The second is based on differential staining of DNA and RNA through co-staining of Hoechst 33342 and Pyronin Y, which is also useful to identify G0 cells from G1 cells. Along with these methods for analyzing cell cycle status, two additional methods for cell proliferation assays with recent updates of newly developed fluorophores, which allow multiplex analysis of cell cycle status, cell proliferation, and a gene of interest using flow cytometry, are outlined.

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