Flow Cytometric Quantification of All Phases of the Cell Cycle and Apoptosis in a Two-color Fluorescence Plot

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2013 Aug 13

PMID 23935867

Citations 63

Authors

Christine Vignon

Christelle Debeissat

Marie-Therese Georget

Didier Bouscary

Emmanuel Gyan

Philippe Rosset

Olivier Herault

Affiliations

Soon will be listed here.

Abstract

An optimal technology for cell cycle analysis would allow the concomitant measurement of apoptosis, G0, G1, S, G2 and M phases in combination with cell surface phenotyping. We have developed an easy method in flow cytometry allowing this discrimination in an only two-color fluorescent plot. It is based on the concomitant use of 7-amino-actinomycin D and the antibodies anti-Ki67 and anti-phospho(Ser10)-histone H3, both conjugated to Alexa Fluor®488 to discriminate G0 and M phases, respectively. The method is particularly valuable in a clinical setting as verified in our laboratory by analyzing human leukemic cells from marrow samples or after exposure to cell cycle modifiers.

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