Ohr Protects Corynebacterium Glutamicum Against Organic Hydroperoxide Induced Oxidative Stress

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2015 Jun 30

PMID 26121694

Citations 15

Authors

Meiru Si

Jianbo Wang

Xiao Xiao

Jingyuan Guan

Yaoling Zhang

Wei Ding

Muhammad Tausif Chaudhry

Yao Wang

Xihui Shen

Affiliations

Soon will be listed here.

Abstract

Ohr, a bacterial protein encoded by the Organic Hydroperoxide Resistance (ohr) gene, plays a critical role in resistance to organic hydroperoxides. In the present study, we show that the Cys-based thiol-dependent Ohr of Corynebacterium glutamicum decomposes organic hydroperoxides more efficiently than hydrogen peroxide. Replacement of either of the two Cys residues of Ohr by a Ser residue resulted in drastic loss of activity. The electron donors supporting regeneration of the peroxidase activity of the oxidized Ohr of C. glutamicum were principally lipoylated proteins (LpdA and Lpd/SucB). A Δohr mutant exhibited significantly decreased resistance to organic hydroperoxides and marked accumulation of reactive oxygen species (ROS) in vivo; protein carbonylation was also enhanced notably. The resistance to hydrogen peroxide also decreased, but protein carbonylation did not rise to any great extent. Together, the results unequivocally show that Ohr is essential for mediation of organic hydroperoxide resistance by C. glutamicum.

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