Molecular Mechanisms of AhpC in Resistance to Oxidative Stress in

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Jul 25

PMID 31338075

Citations 16

Authors

Bing Zhang

Huawei Gu

Yantao Yang

Haonan Bai

Chao Zhao

Meiru Si

Tao Su

Xihui Shen

Affiliations

Soon will be listed here.

Abstract

is a model organism for human pathogens and . The study of peroxiredoxin is helpful for understanding the survival, pathogenic infection, and antibiotic resistance of its homologous species. Alkyl hydroperoxide reductase subunit C (AhpC) is an important peroxiredoxin involved in oxidative damage defense. Here, we report that AhpC exhibits broad specificity for peroxide substrates, including inorganic and organic peroxides and peroxynitrite. AhpC catalyzes the reduction of oxidants using the N-terminal conserved Cys57 as a peroxidatic Cys and the C-terminal conserved Cys171 and Cys173 as resolving Cys. These three conserved Cys residues play critical roles in the catalytic mechanism. AhpD directly interacts with AhpC as an electron donor, and the conserved Cys residues in active site of AhpD are important for AhpC reduction. AhpC is directly repressed by OxyR as shown by identifying the OxyR binding site in the promoter with a DNA binding assay. This work sheds light on the function of AhpC in the peroxides and peroxynitrite damage response in and homologous species.

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