Molecular Screening of Pathogenic Escherichia Coli Strains Isolated from Dairy Neonatal Calves in Cordoba Province, Argentina

Overview

Journal Rev Argent Microbiol

Specialty Microbiology

Date 2015 Jun 1

PMID 26026231

Citations 16

Authors

Natalia Y Picco

Fabrisio E Alustiza

Romina V Bellingeri

Maria C Grosso

Carlos E Motta

Alejandro J Larriestra

Claudina Vissio

Karina I Tiranti

Horacio R Terzolo

Ana R Moreira

Adriana B Vivas

Affiliations

Soon will be listed here.

Abstract

The aim of this study was to perform a current molecular characterization of bovine pathogenic Escherichia coli strains isolated from random samplings in Argentinean dairy farms. Rectal swabs were obtained from 395 (63.7%) healthy and 225 (36.3%) diarrheic calves, belonging to 45 dairy farms in Cordoba Province, Argentina. E. coli isolates were examined for virulence genes (f5, f41, f17, sta, stb, lt, eae, vt) using PCR and the prevalence of E. coli virulence profiles was spatially described in terms of spatial distribution. A total of 30.1% isolates were found to be positive for at least one of the virulence genes. Depending on the different gene combinations present, 11 virulence profiles were found. Most of the isolates analyzed had a single gene, and no combination of fimbrial and enterotoxin gene was predominant. There was no association between the frequency and distribution of E. coli virulence genes and calf health status. Most of the virulence profiles were compatible with ETEC strains and showed a homogeneous distribution over the sampled area. A clustering pattern for E. coli virulence profiles could not be recognized. This work provides updated information on the molecular characterization of pathogenic E. coli strains from dairy herds in Cordoba, Argentina. These findings would be important to formulate prevention programs and effective therapies for diarrhea in calves caused by E. coli.

Citing Articles

Molecular characterization of Escherichia coli virulence markers in neonatal and postweaning piglets from major pig-producing districts of Uganda.

Tusiime M, Mwiine F, Afayoa M, Arojjo S, Erume J BMC Vet Res. 2024; 20(1):230.

PMID: 38802876 PMC: 11129443. DOI: 10.1186/s12917-024-04092-x.

Characteristics, Whole-Genome Sequencing and Pathogenicity Analysis of from a White Feather Broiler Farm.

Wu S, Cui L, Han Y, Lin F, Huang J, Song M Microorganisms. 2023; 11(12).

PMID: 38138083 PMC: 10745608. DOI: 10.3390/microorganisms11122939.

The Diversity of Pathotypes and Vaccination Strategies against This Versatile Bacterial Pathogen.

Pokharel P, Dhakal S, Dozois C Microorganisms. 2023; 11(2).

PMID: 36838308 PMC: 9965155. DOI: 10.3390/microorganisms11020344.

Companion Animals as Potential Reservoirs of Antibiotic Resistant Diarrheagenic in Shandong, China.

Cui L, Zhao X, Li R, Han Y, Hao G, Wang G Antibiotics (Basel). 2022; 11(6).

PMID: 35740235 PMC: 9220070. DOI: 10.3390/antibiotics11060828.

Sandwich-Based Immunosensor for Dual-Mode Detection of Pathogenic F17-Positive Strains.

Salhi I, Rabti A, Dhehibi A, Raouafi N Int J Mol Sci. 2022; 23(11).

PMID: 35682707 PMC: 9181454. DOI: 10.3390/ijms23116028.

References

Bellinzoni R, Blackhall J, Terzolo H, Moreira A, Auza N, Mattion N . Microbiology of diarrhoea in young beef and dairy calves in Argentina. Rev Argent Microbiol. 1990; 22(3):130-6. View

Acha S, Kuhn I, Jonsson P, Mbazima G, Katouli M, Mollby R . Studies on calf diarrhoea in Mozambique: prevalence of bacterial pathogens. Acta Vet Scand. 2004; 45(1-2):27-36. PMC: 1821001. DOI: 10.1186/1751-0147-45-27. View

Bendali F, Bichet H, Schelcher F, Sanaa M . Pattern of diarrhoea in newborn beef calves in south-west France. Vet Res. 1999; 30(1):61-74. View

Cho Y, Han J, Wang C, Cooper V, Schwartz K, Engelken T . Case-control study of microbiological etiology associated with calf diarrhea. Vet Microbiol. 2013; 166(3-4):375-85. PMC: 7117237. DOI: 10.1016/j.vetmic.2013.07.001. View

Younis E, Ahmed A, El-Khodery S, Osman S, El-Naker Y . Molecular screening and risk factors of enterotoxigenic Escherichia coli and Salmonella spp. in diarrheic neonatal calves in Egypt. Res Vet Sci. 2009; 87(3):373-9. PMC: 7111881. DOI: 10.1016/j.rvsc.2009.04.006. View

Ghanbarpour R, Oswald E . Characteristics and virulence genes of Escherichia coli isolated from septicemic calves in southeast of Iran. Trop Anim Health Prod. 2008; 41(7):1091-9. DOI: 10.1007/s11250-008-9289-0. View

Constable P . Antimicrobial use in the treatment of calf diarrhea. J Vet Intern Med. 2004; 18(1):8-17. PMC: 7197454. DOI: 10.1892/0891-6640(2004)18<8:auitto>2.0.co;2. View

Siqueira A, Ribeiro M, Leite D, Tiba M, de Moura C, Lopes M . Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs. Res Vet Sci. 2008; 86(2):206-10. DOI: 10.1016/j.rvsc.2008.07.018. View

Borriello G, Lucibelli M, De Carlo E, Auriemma C, Cozza D, Ascione G . Characterization of enterotoxigenic E. coli (ETEC), Shiga-toxin producing E. coli (STEC) and necrotoxigenic E. coli (NTEC) isolated from diarrhoeic Mediterranean water buffalo calves (Bubalus bubalis). Res Vet Sci. 2011; 93(1):18-22. PMC: 7118792. DOI: 10.1016/j.rvsc.2011.05.009. View

10.

Wasteson Y . Zoonotic Escherichia coli. Acta Vet Scand Suppl. 2002; 95:79-84. View

11.

China B, Pirson V, Mainil J . Typing of bovine attaching and effacing Escherichia coli by multiplex in vitro amplification of virulence-associated genes. Appl Environ Microbiol. 1996; 62(9):3462-5. PMC: 168144. DOI: 10.1128/aem.62.9.3462-3465.1996. View

12.

Tiranti K, Larriestra A, Vissio C, Picco N, Alustiza F, Degioanni A . Prevalence of Cryptosporidium spp. and Giardia spp., spatial clustering and patterns of shedding in dairy calves from Córdoba, Argentina. Rev Bras Parasitol Vet. 2011; 20(2):140-7. DOI: 10.1590/s1984-29612011000200009. View

13.

Guler L, Gunduz K, Ok U . Virulence factors and antimicrobial susceptibility of Escherichia coli isolated from calves in Turkey. Zoonoses Public Health. 2008; 55(5):249-57. DOI: 10.1111/j.1863-2378.2008.01121.x. View

14.

Nagy B, Fekete P . Enterotoxigenic Escherichia coli in veterinary medicine. Int J Med Microbiol. 2005; 295(6-7):443-54. DOI: 10.1016/j.ijmm.2005.07.003. View

15.

Franck S, Bosworth B, Moon H . Multiplex PCR for enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains from calves. J Clin Microbiol. 1998; 36(6):1795-7. PMC: 104926. DOI: 10.1128/JCM.36.6.1795-1797.1998. View

16.

Huasai S, Chen A, Wang C, Li Y, Tongrige B . Occurrence and characteristics of virulence genes of Escherichia coli strains isolated from healthy dairy cows in Inner Mongolia, China. Braz J Microbiol. 2013; 43(2):528-34. PMC: 3768809. DOI: 10.1590/S1517-83822012000200013. View

17.

Kaper J, Nataro J, Mobley H . Pathogenic Escherichia coli. Nat Rev Microbiol. 2004; 2(2):123-40. DOI: 10.1038/nrmicro818. View

18.

Bartels C, Holzhauer M, Jorritsma R, Swart W, Lam T . Prevalence, prediction and risk factors of enteropathogens in normal and non-normal faeces of young Dutch dairy calves. Prev Vet Med. 2009; 93(2-3):162-9. PMC: 7125667. DOI: 10.1016/j.prevetmed.2009.09.020. View

19.

Nguyen T, Vo T, Vu-Khac H . Virulence factors in Escherichia coli isolated from calves with diarrhea in Vietnam. J Vet Sci. 2011; 12(2):159-64. PMC: 3104170. DOI: 10.4142/jvs.2011.12.2.159. View

20.

Garcia A, Ruiz-Santa-Quiteria J, Orden J, Cid D, Sanz R, Gomez-Bautista M . Rotavirus and concurrent infections with other enteropathogens in neonatal diarrheic dairy calves in Spain. Comp Immunol Microbiol Infect Dis. 2000; 23(3):175-83. PMC: 7133995. DOI: 10.1016/s0147-9571(99)00071-5. View