Functional Domains in the Bacteriophage Phi 29 Terminal Protein for Interaction with the Phi 29 DNA Polymerase and with DNA

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1989 Dec 25

PMID 2602154

Citations 20

Authors

A Zaballos

M Salas

Affiliations

Soon will be listed here.

Abstract

Deletion mutants at the amino- and carboxyl-ends of the phi 29 terminal protein, as well as internal deletion and substitution mutants, whose ability to prime the initiation of phi 29 DNA replication was affected to different extent, have been assayed for their capacity to interact with DNA or with the phi 29 DNA polymerase. One DNA binding domain at the amino end of the terminal protein has been mapped. Two regions involved in the binding to the DNA polymerase, an internal region near the amino-terminus and a carboxyl-terminal one, have been also identified. Interaction with both DNA and phi 29 DNA polymerase are required to led to the formation of terminal protein-dAMP initiation complex to start phi 29 DNA replication.

Citing Articles

Coding-Complete Genome Sequence of Staphylococcus aureus Podophage Portland.

Bonasera R, Korn A, Newkirk H, OLeary C, Gill J, Liu M Microbiol Resour Announc. 2019; 8(47).

PMID: 31753959 PMC: 6872901. DOI: 10.1128/MRA.01337-19.

Engineering Permissive Insertion Sites in the Bacteriophage Phi29 DNA-Linked Terminal Protein.

Gella P, Salas M, Mencia M PLoS One. 2016; 11(10):e0164901.

PMID: 27780219 PMC: 5079584. DOI: 10.1371/journal.pone.0164901.

DNA-Binding Proteins Essential for Protein-Primed Bacteriophage Φ29 DNA Replication.

Salas M, Holguera I, Redrejo-Rodriguez M, de Vega M Front Mol Biosci. 2016; 3:37.

PMID: 27547754 PMC: 4974454. DOI: 10.3389/fmolb.2016.00037.

Dissecting the role of the ϕ29 terminal protein DNA binding residues in viral DNA replication.

Holguera I, Munoz-Espin D, Salas M Nucleic Acids Res. 2015; 43(5):2790-801.

PMID: 25722367 PMC: 4357725. DOI: 10.1093/nar/gkv127.

Viral terminal protein directs early organization of phage DNA replication at the bacterial nucleoid.

Munoz-Espin D, Holguera I, Ballesteros-Plaza D, Carballido-Lopez R, Salas M Proc Natl Acad Sci U S A. 2010; 107(38):16548-53.

PMID: 20823229 PMC: 2944753. DOI: 10.1073/pnas.1010530107.

References

Ortin J, Vinuela E, Salas M, Vasquez C . DNA-protein complex in circular DNA from phage phi-29. Nat New Biol. 1971; 234(52):275-7. DOI: 10.1038/newbio234275a0. View

Zaballos A, Lazaro J, Mendez E, Mellado R, Salas M . Effects of internal deletions on the priming activity of the phage phi 29 terminal protein. Gene. 1989; 83(2):187-95. DOI: 10.1016/0378-1119(89)90104-2. View

McDonell M, Simon M, Studier F . Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977; 110(1):119-46. DOI: 10.1016/s0022-2836(77)80102-2. View

Salas M, Mellado R, Vinuela E . Characterization of a protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi29. J Mol Biol. 1978; 119(2):269-91. DOI: 10.1016/0022-2836(78)90438-2. View

Inciarte M, Salas M, Sogo J . Structure of replicating DNA molecules of Bacillus subtilis bacteriophage phi 29. J Virol. 1980; 34(1):187-99. PMC: 288685. DOI: 10.1128/JVI.34.1.187-199.1980. View

Harding N, Ito J . DNA replication of bacteriophage phi 29: characterization of the intermediates and location of the termini of replication. Virology. 1980; 104(2):323-38. DOI: 10.1016/0042-6822(80)90337-2. View

Hermoso J, Salas M . Protein p3 is linked to the DNA of phage phi 29 through a phosphoester bond between serine and 5'-dAMP. Proc Natl Acad Sci U S A. 1980; 77(11):6425-8. PMC: 350297. DOI: 10.1073/pnas.77.11.6425. View

Sogo J, Garcia J, Penalva M, Salas M . Structure of protein-containing replicative intermediates of Bacillus subtilis phage phi 29 DNA. Virology. 1982; 116(1):1-18. DOI: 10.1016/0042-6822(82)90398-1. View

Yoshikawa H, Ito J . Nucleotide sequence of the major early region of bacteriophage phi 29. Gene. 1982; 17(3):323-35. DOI: 10.1016/0378-1119(82)90149-4. View

10.

Escarmis C, Salas M . Nucleotide sequence of the early genes 3 and 4 of bacteriophage phi 29. Nucleic Acids Res. 1982; 10(19):5785-98. PMC: 320930. DOI: 10.1093/nar/10.19.5785. View

11.

Prieto I, Lazaro J, Garcia J, Hermoso J, Salas M . Purification in a functional form of the terminal protein of Bacillus subtilis phage phi 29. Proc Natl Acad Sci U S A. 1984; 81(6):1639-43. PMC: 344973. DOI: 10.1073/pnas.81.6.1639. View

12.

Blanco L, Salas M . Characterization and purification of a phage phi 29-encoded DNA polymerase required for the initiation of replication. Proc Natl Acad Sci U S A. 1984; 81(17):5325-9. PMC: 391696. DOI: 10.1073/pnas.81.17.5325. View

13.

Watabe K, Leusch M, Ito J . Replication of bacteriophage phi 29 DNA in vitro: the roles of terminal protein and DNA polymerase. Proc Natl Acad Sci U S A. 1984; 81(17):5374-8. PMC: 391706. DOI: 10.1073/pnas.81.17.5374. View

14.

Pabo C, Sauer R . Protein-DNA recognition. Annu Rev Biochem. 1984; 53:293-321. DOI: 10.1146/annurev.bi.53.070184.001453. View

15.

Matsumoto K, Saito T, Kim C, Ando T, Hirokawa H . Bacteriophage phi 29 DNA replication in vitro: participation of the terminal protein and the gene 2 product in elongation. Mol Gen Genet. 1984; 196(3):381-6. DOI: 10.1007/BF00436183. View

16.

Pastrana R, Lazaro J, Blanco L, Garcia J, Mendez E, Salas M . Overproduction and purification of protein P6 of Bacillus subtilis phage phi 29: role in the initiation of DNA replication. Nucleic Acids Res. 1985; 13(9):3083-100. PMC: 341222. DOI: 10.1093/nar/13.9.3083. View

17.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

18.

Miller J, McLachlan A, Klug A . Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes. EMBO J. 1985; 4(6):1609-14. PMC: 554390. DOI: 10.1002/j.1460-2075.1985.tb03825.x. View

19.

Blanco L, Salas M . Replication of phage phi 29 DNA with purified terminal protein and DNA polymerase: synthesis of full-length phi 29 DNA. Proc Natl Acad Sci U S A. 1985; 82(19):6404-8. PMC: 390724. DOI: 10.1073/pnas.82.19.6404. View

20.

Hermoso J, Mendez E, Soriano F, Salas M . Location of the serine residue involved in the linkage between the terminal protein and the DNA of phage phi 29. Nucleic Acids Res. 1985; 13(21):7715-28. PMC: 322082. DOI: 10.1093/nar/13.21.7715. View