Effect of Advanced Glycosylation End Products on Apoptosis in Human Adipose Tissue-derived Stem Cells in Vitro

Overview

Journal Cell Biosci

Publisher Biomed Central

Specialty Biology

Date 2015 May 15

PMID 25973170

Citations 18

Authors

Zhe Wang

Hongqiu Li

Dianbao Zhang

Xiaoyu Liu

Feng Zhao

Xining Pang

Qiushi Wang

Affiliations

Soon will be listed here.

Abstract

Background: Both apoptosis and caspase-3 activity in adipose tissue-derived stem cells play an important role in the therapeutic process of diabetes patients. The purpose of this study was to investigate the effect of advanced glycation end products-human serum albumin (AGE-HSA) on apoptosis in human adipose tissue-derived stem cells (ADSCs) and to characterize the signal transduction pathways activated by AGEs that are involved in apoptosis regulation.

Results: AGE-HSA promoted apoptosis and caspase-3 activity in ADSCs. However, the effects of AGE-HSA were significantly attenuated by an inhibitor of p38 MAPK, but not by inhibitors of JNK MAPK or ERK MAPK. AGE-HSA also upregulated the expression of RAGE. Silencing of the RAGE gene inhibited AGE-HSA-induced apoptosis, and activation and expression of phosphorylated p38 MAPK.

Conclusions: These results suggest that AGE-HSA promote the apoptosis of ADSCs in vitro via a RAGE-dependent p38 MAPK pathway.

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