Electric LAMP: Virtual Loop-Mediated Isothermal AMPlification

Overview

Journal ISRN Bioinform

Specialty Biology

Date 2015 May 14

PMID 25969751

Citations 8

Authors

Nelson R Salinas

Damon P Little

Affiliations

Soon will be listed here.

Abstract

We present eLAMP, a PERL script, with Tk graphical interface, that electronically simulates Loop-mediated AMPlification (LAMP) allowing users to efficiently test putative LAMP primers on a set of target sequences. eLAMP can match primers to templates using either exact (via builtin PERL regular expressions) or approximate matching (via the tre-agrep library). Performance was tested on 40 whole genome sequences of Staphylococcus. eLAMP correctly predicted that the two tested primer sets would amplify from S. aureus genomes and not amplify from other Staphylococcus species. Open source (GNU Public License) PERL scripts are available for download from the New York Botanical Garden's website.

Citing Articles

Loop-mediated isothermal amplification assay detects multiple alleles of genes in and other Gram-negative bacteria despite primer-template mismatches.

Carascal M, Macalalad L, Petronio-Santos J, Destura R, Rivera W Heliyon. 2024; 10(15):e35653.

PMID: 39170108 PMC: 11337129. DOI: 10.1016/j.heliyon.2024.e35653.

Reverse transcription loop-mediated isothermal amplification (RT-LAMP) primer design based on Indonesia SARS-CoV-2 RNA sequence.

Ibadurrahman I, Suryani , Desriani J Genet Eng Biotechnol. 2023; 21(1):168.

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Isothermal nucleic acid amplification and its uses in modern diagnostic technologies.

Srivastava P, Prasad D 3 Biotech. 2023; 13(6):200.

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Loop Mediated Isothermal Amplification: Principles and Applications in Plant Virology.

Panno S, Matic S, Tiberini A, Caruso A, Bella P, Torta L Plants (Basel). 2020; 9(4).

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