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Loss of Proliferative Capacity in Immunohemopoietic Stem Cells Caused by Serial Transplantation Rather Than Aging

Overview
Journal J Exp Med
Specialties Allergy & Immunology
General Medicine
Date 1978 May 1
PMID 25943
Citations 56
Authors
D E Harrison
C M Astle
J A DeLaittre
Affiliations
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Abstract

Marrow stem cell lines from old donors and those from young controls gave equally rapid rates of colony growth on spleens of irradiated mice. Old and young stem cell lines competed equally well with chromosomally marked marrow stem cells from a young donor in producing cell types that are stimulated by bleeding; old cells competed 70% as well as young in producing cell types stimulated by phytohemagglutinin (PHA) in vitro. After a single serial transplantation, the rates of colony growth declined 1.5- to 2.5-fold, and the ability to compete declined 2- to 4-fold for bleeding-stimulated and 4- to 10-fold for PHA-stimulated cells. Thus, immediate stem cell proliferative capacities decline much more after one serial transplantation than after a lifetime of normal function.

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References
1.
Harrison D . Normal function of transplanted marrow cell lines from aged mice. J Gerontol. 1975; 30(3):279-85. DOI: 10.1093/geronj/30.3.279. View
2.
Till J, McCulloch E . A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res. 1961; 14:213-22. View
3.
CUDKOWICZ G, UPTON A, Shearer G, HUGHES W . LYMPHOCYTE CONTENT AND PROLIFERATIVE CAPACITY OF SERIALLY TRANSPLANTED MOUSE BONE MARROW. Nature. 1964; 201:165-7. DOI: 10.1038/201165a0. View
4.
Siminovitch L, Till J, McCulloch E . DECLINE IN COLONY-FORMING ABILITY OF MARROW CELLS SUBJECTED TO SERIAL TRANSPLANTATION INTO IRRADIATED MICE. J Cell Comp Physiol. 1964; 64:23-31. DOI: 10.1002/jcp.1030640104. View
5.
Harrison D, Astle C, Doubleday J . Cell lines from old immunodeficient donors give normal responses in young recipients. J Immunol. 1977; 118(4):1223-7. View