Transcriptomic Profiles of the Smoke Tree Wilt Fungus Verticillium Dahliae Under Nutrient Starvation Stresses

Overview

Journal Mol Genet Genomics

Specialty Genetics

Date 2015 May 6

PMID 25939502

Citations 8

Authors

Dianguang Xiong

Yonglin Wang

Chengming Tian

Affiliations

Soon will be listed here.

Abstract

Verticillium dahliae is a notorious plant pathogen that causes vascular wilt on more than 200 plant species. During plant infection, efficient pathogen nutrition during the interaction with the host is a requisite for successful infection. However, little attention has been focused on nutrient uptake and starvation responses in this fungus. Here, we used RNA-Seq to analyze the response of V. dahliae to nutrient starvation, including carbon and nitrogen depletion. Gene expression profile analysis showed that 1854 genes were differentially expressed under carbon starvation (852 upregulated and 539 downregulated genes) and nitrogen starvation (487 upregulated and 291 downregulated genes). Among the differentially expressed genes, genes involved in utilization or production acetyl-CoA, including glycolysis, fatty acid biosynthesis or metabolism, and melanin biosynthesis, were repressed under carbon starvation, whereas melanin biosynthesis genes were strongly induced under nitrogen starvation. These results, combined with VDH1 expression data, suggested that melanin biosynthesis and microsclerotia development were induced under nitrogen starvation, but microsclerotia development was suppressed under carbon starvation. Furthermore, many genes encoding carbohydrate-active enzymes and secreted proteins were induced under carbon starvation. Overall, the results improve our understanding of the response of V. dahliae to nutrient starvation and help to identify potential virulence factors for the development of novel disease control strategies.

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References

Yi M, Valent B . Communication between filamentous pathogens and plants at the biotrophic interface. Annu Rev Phytopathol. 2013; 51:587-611. DOI: 10.1146/annurev-phyto-081211-172916. View

Ozcan S, Johnston M . Function and regulation of yeast hexose transporters. Microbiol Mol Biol Rev. 1999; 63(3):554-69. PMC: 103746. DOI: 10.1128/MMBR.63.3.554-569.1999. View

Wilson R, Arst Jr H . Mutational analysis of AREA, a transcriptional activator mediating nitrogen metabolite repression in Aspergillus nidulans and a member of the "streetwise" GATA family of transcription factors. Microbiol Mol Biol Rev. 1998; 62(3):586-96. PMC: 98926. DOI: 10.1128/MMBR.62.3.586-596.1998. View

Alves Jr S, Herberts R, Hollatz C, Trichez D, Miletti L, de Araujo P . Molecular analysis of maltotriose active transport and fermentation by Saccharomyces cerevisiae reveals a determinant role for the AGT1 permease. Appl Environ Microbiol. 2008; 74(5):1494-501. PMC: 2258630. DOI: 10.1128/AEM.02570-07. View

Klosterman S, Subbarao K, Kang S, Veronese P, Gold S, Thomma B . Comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens. PLoS Pathog. 2011; 7(7):e1002137. PMC: 3145793. DOI: 10.1371/journal.ppat.1002137. View

Dowzer C, Kelly J . Analysis of the creA gene, a regulator of carbon catabolite repression in Aspergillus nidulans. Mol Cell Biol. 1991; 11(11):5701-9. PMC: 361941. DOI: 10.1128/mcb.11.11.5701-5709.1991. View

Marzluf G . Genetic regulation of nitrogen metabolism in the fungi. Microbiol Mol Biol Rev. 1997; 61(1):17-32. PMC: 232598. DOI: 10.1128/mmbr.61.1.17-32.1997. View

Tzima A, Paplomatas E, Tsitsigiannis D, Kang S . The G protein β subunit controls virulence and multiple growth- and development-related traits in Verticillium dahliae. Fungal Genet Biol. 2012; 49(4):271-83. DOI: 10.1016/j.fgb.2012.02.005. View

Tran V, Braus-Stromeyer S, Kusch H, Reusche M, Kaever A, Kuhn A . Verticillium transcription activator of adhesion Vta2 suppresses microsclerotia formation and is required for systemic infection of plant roots. New Phytol. 2014; 202(2):565-581. DOI: 10.1111/nph.12671. View

10.

Coradetti S, Craig J, Xiong Y, Shock T, Tian C, Glass N . Conserved and essential transcription factors for cellulase gene expression in ascomycete fungi. Proc Natl Acad Sci U S A. 2012; 109(19):7397-402. PMC: 3358856. DOI: 10.1073/pnas.1200785109. View

11.

Amselem J, Cuomo C, van Kan J, Viaud M, Benito E, Couloux A . Genomic analysis of the necrotrophic fungal pathogens Sclerotinia sclerotiorum and Botrytis cinerea. PLoS Genet. 2011; 7(8):e1002230. PMC: 3158057. DOI: 10.1371/journal.pgen.1002230. View

12.

Klimes A, Amyotte S, Grant S, Kang S, Dobinson K . Microsclerotia development in Verticillium dahliae: Regulation and differential expression of the hydrophobin gene VDH1. Fungal Genet Biol. 2008; 45(12):1525-32. DOI: 10.1016/j.fgb.2008.09.014. View

13.

Staples R . Research on the Rust Fungi During the Twentieth Century. Annu Rev Phytopathol. 2001; 38:49-69. DOI: 10.1146/annurev.phyto.38.1.49. View

14.

Znameroski E, Glass N . Using a model filamentous fungus to unravel mechanisms of lignocellulose deconstruction. Biotechnol Biofuels. 2013; 6(1):6. PMC: 3598899. DOI: 10.1186/1754-6834-6-6. View

15.

Coleman M, Henricot B, Arnau J, Oliver R . Starvation-induced genes of the tomato pathogen Cladosporium fulvum are also induced during growth in planta. Mol Plant Microbe Interact. 1997; 10(9):1106-9. DOI: 10.1094/MPMI.1997.10.9.1106. View

16.

Xiong D, Wang Y, Ma J, Klosterman S, Xiao S, Tian C . Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae. BMC Genomics. 2014; 15:324. PMC: 4035056. DOI: 10.1186/1471-2164-15-324. View

17.

Soanes D, Chakrabarti A, Paszkiewicz K, Dawe A, Talbot N . Genome-wide transcriptional profiling of appressorium development by the rice blast fungus Magnaporthe oryzae. PLoS Pathog. 2012; 8(2):e1002514. PMC: 3276559. DOI: 10.1371/journal.ppat.1002514. View

18.

Jiang C, Zhang S, Zhang Q, Tao Y, Wang C, Xu J . FgSKN7 and FgATF1 have overlapping functions in ascosporogenesis, pathogenesis and stress responses in Fusarium graminearum. Environ Microbiol. 2014; 17(4):1245-60. DOI: 10.1111/1462-2920.12561. View

19.

Bailey A, Mueller E, Bowyer P . Ornithine decarboxylase of Stagonospora (Septoria) nodorum is required for virulence toward wheat. J Biol Chem. 2000; 275(19):14242-7. DOI: 10.1074/jbc.275.19.14242. View

20.

Klosterman S, Atallah Z, Vallad G, Subbarao K . Diversity, pathogenicity, and management of verticillium species. Annu Rev Phytopathol. 2009; 47:39-62. DOI: 10.1146/annurev-phyto-080508-081748. View