Mixed Serum-deprived and Normal Adipose-derived Mesenchymal Stem Cells Against Acute Lung Ischemia-reperfusion Injury in Rats

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2015 Apr 23

PMID 25901192

Citations 16

Authors

Cheuk-Kwan Sun

Steve Leu

Shu-Yuan Hsu

Yen-Yi Zhen

Li-Teh Chang

Ching-Yen Tsai

Yung-Lung Chen

Yen-Ta Chen

Tzu-Hsien Tsai

Fan-Yen Lee

Jiunn-Jye Sheu

Hsueh-Wen Chang

Hon-Kan Yip

Affiliations

Soon will be listed here.

Abstract

Background: This study hypothesized that combined serum-deprived (Sd) and healthy (He) adipose-derived mesenchymal stem cell (ADMSC) therapy is superior to either alone in reducing acute lung ischemia-reperfusion injury (ALIRI).

Methods: Adult male Sprague-Dawley (SD) rats (n = 30) were equally randomized into group 1 (sham control), group 2 (ALIRI + culture medium), group 3 (ALIRI + intravenous autologous 1.2 × 10(6) He-ADMSCs at 30 minute, 6 h, and 24 h following lung ischemia/reperfusion for 45 minutes/72 hours, respectively), group 4 (ALIRI + 1.2 × 10(6) Sd-ADMSCs at identical time points after ischemia/reperfusion), and group 5 (ALIRI + 1.2 × 10(6) combined Sd-ADMSC/He-ADMSC 1:1).

Results: Blood oxygen saturation (%) was lowest in group 2, lower in groups 3 to 5 than in group 1, and lower in group 5 than in group 1, whereas right ventricular systolic pressure (RVSP) showed a reverse pattern among the five groups (all p < 0.001). Additionally, changes in histological scoring of lung parenchymal damage, inflammatory and apoptotic biomarkers showed identical pattern compared to that of RVSP in all groups (all p < 0.001). Protein expressions of VCAM-1, ICAM-1, oxidative stress, TNF-α, nuclear factor-κB, and number of CD68 + cells were highest in group 2, higher in groups 3 to 5 than in group 1, and higher in groups 3 and 4 than in group 5, whereas NQO-1 and HO-1 activities and number of CD31 + and vWF + cells showed opposite changes compared with those of inflammatory biomarkers (all p < 0.001).

Conclusion: Combined Sd-ADMSC/He-ADMSC therapy offered superior benefit to either option alone in minimizing rodent ALIRI through suppressing oxidative stress and inflammatory reaction.

Citing Articles

Adipose-derived stem cells ameliorate radiation-induced lung injury by activating the DDAH1/ADMA/eNOS signaling pathway.

Fu Q, Gao Q, Jiao S, Da F, Guo J, Liu Y Regen Ther. 2024; 27:398-407.

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Prion Protein Overexpression in Adipose-Derived Mesenchymal Stem Cells (ADMSCs) Effectively Protected Rodent Kidney Against Ischemia-Reperfusion Injury Via Enhancing ATP/Mitochondrial Biogenesis-Role of ADMSC Rejuvenation and Proliferation.

Chen Y, Yang C, Chiang J, Sung P, Shao P, Huang C Cell Transplant. 2023; 32:9636897231211067.

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Interplay Between Inflammatory-immune and Interleukin-17 Signalings Plays a Cardinal Role on Liver Ischemia-reperfusion Injury-Synergic Effect of IL-17Ab, Tacrolimus and ADMSCs on Rescuing the Liver Damage.

Ko S, Li Y, Shao P, Chiang J, Sung P, Chen Y Stem Cell Rev Rep. 2023; 19(8):2852-2868.

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Jagged/Notch proteins promote endothelial-mesenchymal transition-mediated pulmonary arterial hypertension via upregulation of the expression of GATAs.

Lin K, Yeh J, Shao P, Chiang J, Sung P, Huang C J Cell Mol Med. 2023; 27(8):1110-1130.

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Intrarenal arterial administration of human umbilical cord-derived mesenchymal stem cells effectively preserved the residual renal function of diabetic kidney disease in rat.

Yue Y, Yeh J, Chiang J, Sung P, Chen Y, Liu F Stem Cell Res Ther. 2022; 13(1):186.

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