Enhanced Vascularization in Hybrid PCL/gelatin Fibrous Scaffolds with Sustained Release of VEGF

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2015 Apr 18

PMID 25883978

Citations 18

Authors

Kai Wang

Xuejiao Chen

Yiwa Pan

Yun Cui

Xin Zhou

Deling Kong

Qiang Zhao

Affiliations

Soon will be listed here.

Abstract

Creating a long-lasting and functional vasculature represents one of the most fundamental challenges in tissue engineering. VEGF has been widely accepted as a potent angiogenic factor involved in the early stages of blood vessel formation. In this study, fibrous scaffolds that consist of PCL and gelatin fibers were fabricated. The gelatin fibers were further functionalized by heparin immobilization, which provides binding sites for VEGF and thus enables the sustained release of VEGF. In vitro release test confirms the sustained releasing profile of VEGF, and stable release was observed over a time period of 25 days. In vitro cell assay indicates that VEGF release significantly promoted the proliferation of endothelial cells. More importantly, in vivo subcutaneous implantation reflects that vascularization has been effectively enhanced in the PCL/gelatin scaffolds compared with the PCL counterpart due to the sustained release of VEGF. Therefore, the heparinized PCL/gelatin scaffolds developed in this study may be a promising candidate for regeneration of complex tissues with sufficient vascularization.

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References

Pieper J, Hafmans T, van Wachem P, Van Luyn M, Brouwer L, Veerkamp J . Loading of collagen-heparan sulfate matrices with bFGF promotes angiogenesis and tissue generation in rats. J Biomed Mater Res. 2002; 62(2):185-94. DOI: 10.1002/jbm.10267. View

Kawamoto A, Asahara T, Losordo D . Transplantation of endothelial progenitor cells for therapeutic neovascularization. Cardiovasc Radiat Med. 2003; 3(3-4):221-5. DOI: 10.1016/s1522-1865(03)00082-9. View

Druecke D, Langer S, Lamme E, Pieper J, Ugarkovic M, Steinau H . Neovascularization of poly(ether ester) block-copolymer scaffolds in vivo: long-term investigations using intravital fluorescent microscopy. J Biomed Mater Res A. 2003; 68(1):10-8. DOI: 10.1002/jbm.a.20016. View

Guan R, Sun X, Hou S, Wu P, Chaikof E . A glycopolymer chaperone for fibroblast growth factor-2. Bioconjug Chem. 2004; 15(1):145-51. DOI: 10.1021/bc034138t. View

McAvoy T . The biologic half-life of heparin. Clin Pharmacol Ther. 1979; 25(3):372-9. DOI: 10.1002/cpt1979253372. View

Lijnen H, Hoylaerts M, Collen D . Heparin binding properties of human histidine-rich glycoprotein. Mechanism and role in the neutralization of heparin in plasma. J Biol Chem. 1983; 258(6):3803-8. View

Prestrelski S, Fox G, Arakawa T . Binding of heparin to basic fibroblast growth factor induces a conformational change. Arch Biochem Biophys. 1992; 293(2):314-9. DOI: 10.1016/0003-9861(92)90401-h. View

Walker A, Turnbull J, Gallagher J . Specific heparan sulfate saccharides mediate the activity of basic fibroblast growth factor. J Biol Chem. 1994; 269(2):931-5. View

Lyon M, Rushton G, Gallagher J . The interaction of the transforming growth factor-betas with heparin/heparan sulfate is isoform-specific. J Biol Chem. 1997; 272(29):18000-6. DOI: 10.1074/jbc.272.29.18000. View

10.

Karageorgiou V, Kaplan D . Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials. 2005; 26(27):5474-91. DOI: 10.1016/j.biomaterials.2005.02.002. View

11.

Nillesen S, Geutjes P, Wismans R, Schalkwijk J, Daamen W, van Kuppevelt T . Increased angiogenesis and blood vessel maturation in acellular collagen-heparin scaffolds containing both FGF2 and VEGF. Biomaterials. 2006; 28(6):1123-31. DOI: 10.1016/j.biomaterials.2006.10.029. View

12.

Chung H, Kim H, Yoon J, Park T . Heparin immobilized porous PLGA microspheres for angiogenic growth factor delivery. Pharm Res. 2006; 23(8):1835-41. DOI: 10.1007/s11095-006-9039-9. View

13.

Huang M, Vitharana S, Peek L, Coop T, Berkland C . Polyelectrolyte complexes stabilize and controllably release vascular endothelial growth factor. Biomacromolecules. 2007; 8(5):1607-14. DOI: 10.1021/bm061211k. View

14.

Johnson P, Mikos A, Fisher J, Jansen J . Strategic directions in tissue engineering. Tissue Eng. 2007; 13(12):2827-37. DOI: 10.1089/ten.2007.0335. View

15.

Rouwkema J, Rivron N, van Blitterswijk C . Vascularization in tissue engineering. Trends Biotechnol. 2008; 26(8):434-41. DOI: 10.1016/j.tibtech.2008.04.009. View

16.

Wang S, Zhang Y, Wang H, Yin G, Dong Z . Fabrication and properties of the electrospun polylactide/silk fibroin-gelatin composite tubular scaffold. Biomacromolecules. 2009; 10(8):2240-4. DOI: 10.1021/bm900416b. View

17.

Sun B, Chen B, Zhao Y, Sun W, Chen K, Zhang J . Crosslinking heparin to collagen scaffolds for the delivery of human platelet-derived growth factor. J Biomed Mater Res B Appl Biomater. 2009; 91(1):366-72. DOI: 10.1002/jbm.b.31411. View

18.

Ju Y, Choi J, Atala A, Yoo J, Lee S . Bilayered scaffold for engineering cellularized blood vessels. Biomaterials. 2010; 31(15):4313-21. DOI: 10.1016/j.biomaterials.2010.02.002. View

19.

Chung Y, Kim S, Lee Y, Park S, Cho K, Yuk S . Efficient revascularization by VEGF administration via heparin-functionalized nanoparticle-fibrin complex. J Control Release. 2010; 143(3):282-9. DOI: 10.1016/j.jconrel.2010.01.010. View

20.

Conconi M, Ghezzo F, Dettin M, Urbani L, Grandi C, Guidolin D . Effects on in vitro and in vivo angiogenesis induced by small peptides carrying adhesion sequences. J Pept Sci. 2010; 16(7):349-57. DOI: 10.1002/psc.1251. View